【摘要】：為研究日本血吸蟲高遷移率族蛋白(Schistosoma japonicum high mobility group box1,Sj HMGB1)體外活化小鼠巨噬細(xì)胞的功能,利用真核重組Sj HMGB1蛋白與巨噬細(xì)胞共培養(yǎng)48 h,流式細(xì)胞術(shù)檢測(cè)巨噬細(xì)胞表面標(biāo)志分子以及趨化因子受體的表達(dá)。收集Sj HMGB1蛋白與巨噬細(xì)胞共孵育48 h后上清,ELISA檢測(cè)上清中TNF-α與IL-10的含量,Griess法檢測(cè)上清中NO的含量。流式結(jié)果顯示,與對(duì)照組相比,Sj HMGB1蛋白刺激組的巨噬細(xì)胞表面MHC-Ⅱ分子,TLR4受體以及趨化因子受體CCR7的表達(dá)顯著上調(diào)且差異具有極顯著統(tǒng)計(jì)學(xué)意義(P0.01),TLR2受體的表達(dá)顯著下調(diào),差異具有顯著統(tǒng)計(jì)學(xué)意義(P0.05)。ELISA結(jié)果顯示,Sj HMGB1能夠刺激巨噬細(xì)胞分泌致炎因子TNF-α,未能促進(jìn)抑炎因子IL-10的釋放。Griess法表明Sj HMGB1能夠促進(jìn)巨噬細(xì)胞分泌NO。本研究結(jié)果表明Sj HMGB1可能通過TLR4通路活化小鼠巨噬細(xì)胞并誘導(dǎo)其向致炎性M1型巨噬細(xì)胞極化。
[Abstract]:In order to study the activation of mouse macrophages with high mobility group protein (Schistosoma japonicum high mobility group box1,Sj HMGB1 of Schistosoma japonicum in vitro, the recombinant Sj HMGB1 protein was co-cultured with macrophages for 48 h. The expression of macrophage surface markers and chemokine receptors were detected by flow cytometry. After incubated with macrophages for 48 hours, Sj HMGB1 protein was collected. The contents of TNF- 偽 and IL-10 in the supernatant were detected by ELISA, and the content of NO in the supernatant was detected by Griess. The results of flow cytometry showed that the expression of MHC- 鈪，

本文編號(hào)：2477519