【摘要】：本試驗(yàn)擬通過以頸靜脈灌注氨基酸混合液的方式,探索頸靜脈與陰外動(dòng)脈中氨基酸濃度和組成比例的關(guān)系。將3頭體重、胎次、泌乳期、泌乳量和體況基本一致的荷斯坦奶牛按3×3拉丁方試驗(yàn)設(shè)計(jì),以不同的3種氨基酸混合液分3個(gè)周期進(jìn)行頸靜脈灌注,每期22 d(7 d灌注期+15 d間隔期)。在每個(gè)灌注期的7 d采集血樣,在當(dāng)天灌注開始后的0、0.5、1、2、4 h進(jìn)行采樣。結(jié)果表明:灌注液所含的6種氨基酸在頸靜脈和陰外動(dòng)脈中的濃度呈三次方相關(guān),并在一定范圍內(nèi)呈線性相關(guān);其他所測(cè)的氨基酸除了天冬氨酸和酪氨酸之外,也具有三次方相關(guān)及一定范圍內(nèi)線性相關(guān);頸靜脈和陰外動(dòng)脈中的氨基酸組成比例也具有相關(guān)性。綜上,除了天冬氨酸和酪氨酸外,頸靜脈與陰外動(dòng)脈氨基酸的濃度和組成比例具有一定的相關(guān)性,可采用頸靜脈灌注替代陰外動(dòng)脈灌注進(jìn)行相關(guān)種類氨基酸的研究。
[Abstract]:The purpose of this study was to explore the relationship between amino acid concentration and composition ratio between jugular vein and external vulvar artery by injecting amino acid mixture into jugular vein. Three Holstein cows with body weight, gestation, lactation stage, lactation volume and body condition were designed according to the 3 脳 3 Latin square test and perfused into jugular vein with different amino acid mixture for three cycles. Each period was 22 days (7 days, 15 days interval). Blood samples were collected on the 7th day of each perfusion period, and were sampled at 0, 0.5, 1, 2, 4 hours after the beginning of perfusion on the same day. The results showed that the concentrations of 6 amino acids in jugular vein and external pudendal artery were positively correlated with each other in a certain range. In addition to aspartic acid and tyrosine, other amino acids also have third-order correlation and linear correlation within a certain range, as well as the proportion of amino acids in jugular vein and vulvar artery. Besides aspartic acid and tyrosine, the amino acid concentration and composition ratio of jugular vein and vulvar artery were correlated to some extent. Jugular vein perfusion could be used as a substitute for perineal artery perfusion to study the related amino acids.
【作者單位】： 揚(yáng)州大學(xué)動(dòng)物科學(xué)與技術(shù)學(xué)院;揚(yáng)州市揚(yáng)大康源乳業(yè)有限公司;
【基金】：江蘇省自然科學(xué)基金項(xiàng)目(BK20141270、BK20151312) 揚(yáng)州市產(chǎn)學(xué)研協(xié)同創(chuàng)新項(xiàng)目(SXT20140012) 江蘇省優(yōu)勢(shì)學(xué)科(PAPD)
【分類號(hào)】：S823.5

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本文編號(hào)：2461246