外源酶對湖羊瘤胃發(fā)酵的影響
[Abstract]:The aim of this study was to study the effects of exogenous enzymes on the in vitro culture of microorganisms in rumen fluid of Hu sheep. The exogenous enzymes were prepared by cellulase and xylanase in the proportion of 4:1. The effect of exogenous enzyme on rumen liquid culture in vitro was studied in order to provide theoretical basis for the development of exogenous enzyme as feed additive for Hu sheep. Four levels of exogenous enzyme (10 mg / kg, 20 mg / kg, 30mg/kg and 40mg/kg) were studied in vitro culture of Hu sheep rumen fluid. The concentrations of pH value, ammonia nitrogen (NH3-N) and ammonia nitrogen (NH3-N) in culture medium were cultured for 6 h, 12 h, 18 h, 24 h, 36 h and 48 h, respectively, at the levels of 10 mg / kg, 20 mg / kg, 6 h, 12 h, 18 h, 24 h, respectively. The effect of microbial protein (MCP) concentration and volatile fatty acid (VFA) concentration. The results showed that adding different levels of exogenous enzymes could improve the characteristics of rumen microbial culture in vitro, in which the addition of 20mg/kg was the best, and the VFA was 37% higher than that of the control group (P0.01). The concentration of NH3-N was increased by 17% (P0.01) compared with the control group (P0.01), 8% higher than that of the control group (P0.01). It can be seen that the addition of exogenous enzymes can improve the fermentation characteristics of rumen microorganisms.
【學位授予單位】:福建農(nóng)林大學
【學位級別】:碩士
【學位授予年份】:2016
【分類號】:S826.5
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