【摘要】：研究了以豆粕(添加2%葡萄糖)為基質(zhì)的植物乳桿菌固態(tài)發(fā)酵菌劑制備的最佳條件。以干燥后活菌數(shù)為指標(biāo),通過(guò)單因素和Box-Behnken響應(yīng)面實(shí)驗(yàn)設(shè)計(jì)對(duì)發(fā)酵工藝進(jìn)行優(yōu)化,對(duì)豆粕發(fā)酵前后的主要營(yíng)養(yǎng)成分及抗?fàn)I養(yǎng)因子含量進(jìn)行測(cè)定,并最終確定了烘干條件。結(jié)果表明,在發(fā)酵溫度30.6℃、接種量4.45%、加水比1∶0.61、發(fā)酵時(shí)間46.40 h時(shí),活菌數(shù)為最高達(dá)到9.94 lg(CFU/g);粗蛋白、酸溶蛋白、游離氨基酸總量和蛋白質(zhì)消化率較發(fā)酵前分別提高了4.55%、1.24%、50.47 mg/g和4.21%;胰蛋白酶抑制因子、脲酶和植酸較發(fā)酵前分別降低了94.59%、91.64%和30.22%;最佳干燥條件為熱風(fēng)干燥50℃、4 h,菌劑活菌總數(shù)為9.88 lg(CFU/g)。
[Abstract]:The optimum conditions for the preparation of solid-state fermentation agent of Lactobacillus plantarum based on soybean meal (2% glucose) were studied. Taking the number of living bacteria after drying as an index, the fermentation process was optimized by single factor and Box-Behnken response surface experiment design. The main nutrient composition and anti-nutritive factor content of soybean meal before and after fermentation were determined, and the drying conditions were finally determined. The results showed that when the fermentation temperature was 30.6 鈩，

本文編號(hào)：2446284