【摘要】：對產后健康奶牛陰道的乳酸菌進行分離篩選并對乳酸菌功能特性進行研究。采用傳統(tǒng)方法對健康奶牛陰道內乳酸菌進行分離,通過抑菌試驗對乳酸菌分離株進行篩選,并研究其產酸性能、抗生素敏感性,通過16SrDNA序列分析方法對分離株進行鑒定。最后獲得4株乳酸菌分離株,經鑒定分別為唾液乳桿菌、糞腸球菌、屎腸球菌和巴黎鏈球菌。4株菌株均可作為防治奶牛子宮內膜炎潛在的益生菌使用。
[Abstract]:Lactobacillus was isolated and screened from the vagina of healthy cows after delivery and the functional characteristics of lactobacillus were studied. Lactobacillus isolated from vagina of healthy dairy cows was isolated by traditional method. The isolated strains were screened by bacteriostatic test, and their acid production performance and antibiotic sensitivity were studied. The isolated strains were identified by 16SrDNA sequence analysis. Finally, four strains of lactobacillus were isolated and identified as Lactobacillus saliva, Enterococcus faecalis, Enterococcus faecium and Streptococcus Paris. All four strains could be used as potential probiotics for the prevention and treatment of endometritis in dairy cows.
【作者單位】： 吉林農業(yè)大學動物科學技術學院;
【基金】：吉林省重大科技攻關項目(20140203016NY;20140203013NY) 吉林省科技攻關重點項目(2015-0204074NY) 吉林省科技創(chuàng)新團隊(20150519018JH)
【分類號】：S823;S852.6

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