【摘要】：為了掌握胞內(nèi)勞森菌在湖北省5個(gè)疑似豬增生性腸炎暴發(fā)規(guī)�；i場(chǎng)中的流行情況,我們建立了一種從糞便中檢測(cè)胞內(nèi)勞森菌的巢式PCR方法,并利用該方法對(duì)采集于這5個(gè)規(guī)�；i場(chǎng)不同生產(chǎn)階段不同臨床表現(xiàn)豬群的1 003份糞便樣品進(jìn)行監(jiān)測(cè)。結(jié)果顯示:5個(gè)豬場(chǎng)的胞內(nèi)勞森菌檢出率在11.5%~19.4%之間,平均陽性率為15.5%;就臨床表現(xiàn)而言,腹瀉豬糞便樣品中胞內(nèi)勞森菌的檢出率高于非腹瀉豬;就生產(chǎn)階段而言,保育及育肥豬中胞內(nèi)勞森菌的檢出率高于斷奶仔豬、母豬和公豬。我們還利用糞便檢測(cè)胞內(nèi)勞森菌呈陽性、疑似臨床癥狀明顯的豬的腸道制備的感染溶液進(jìn)行了病例復(fù)制,試驗(yàn)豬在灌服感染溶液后出現(xiàn)了與胞內(nèi)勞森菌感染相似的臨床癥狀及病理變化,并且從感染豬的腸道組織和糞便中檢測(cè)到了胞內(nèi)勞森菌的存在。本試驗(yàn)對(duì)于進(jìn)一步了解胞內(nèi)勞森菌在我國豬群中的流行情況及致病性具有一定的意義。
[Abstract]:In order to understand the prevalence of intracellular Lawson in five pig farms with suspected swine proliferative enteritis outbreak in Hubei Province, we established a nested PCR method for detection of intracellular Lawson from feces. The method was used to monitor 1 003 fecal samples collected at different production stages and different clinical manifestations in five large-scale pig farms. The results showed that the detection rate of intracellular Lawsonella in 5 pig farms was between 11.5% and 19.4%, the average positive rate was 15.5%, the detection rate of intracellular Lawson in fecal samples of diarrhoeal pigs was higher than that of non-diarrhoeal pigs, and the positive rate in fecal samples of diarrhea pigs was higher than that in non-diarrhoeal pigs. In the production stage, the detection rate of intracellular Lawsonella in conservation and finishing pigs was higher than that in weaning piglets, sows and boars. We also used fecal tests to detect the presence of intracellular Lawsonella positive, suspected to have obvious clinical symptoms of swine intestinal tract prepared infection solution for the replication of the case. The clinical symptoms and pathological changes similar to that of intracellular Lawsonella infection were observed in the experimental pigs after infusing the infected solution, and the existence of intracellular Lawson bacteria was detected in the intestinal tissue and feces of the infected pigs. The results showed that the clinical symptoms and pathological changes of the infected pigs were similar to those of the infected pigs. This study is of great significance for further understanding the epidemic situation and pathogenicity of intracellular Lawsonella in pig populations in China.
【作者單位】： 華中農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)院/農(nóng)業(yè)微生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室/生豬健康養(yǎng)殖協(xié)同創(chuàng)新中心;
【基金】：國家科技支撐計(jì)劃資助項(xiàng)目(2014BAD20B00) 湖北省科技支撐計(jì)劃資助項(xiàng)目(2013BBB14)
【分類號(hào)】：S852.61
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本文編號(hào)：2434756