【摘要】：試驗(yàn)旨在研究急性熱應(yīng)激初期血清生化指標(biāo)及與耐熱性能的關(guān)系,為篩選肉兔耐熱性評(píng)價(jià)指標(biāo)提供理論依據(jù)。選擇同期出生、體重相近的健康4月齡新西蘭兔55只,隨機(jī)分配在2個(gè)人工溫控室內(nèi),其中適溫對(duì)照組環(huán)境溫度為(21±1)℃(n=25),高溫?zé)釕?yīng)激試驗(yàn)組為(38±1)℃(n=30)。準(zhǔn)確記錄試驗(yàn)組中每只兔的耐熱時(shí)間,并在熱應(yīng)激起始0 h(HS0)和熱應(yīng)激8 h(HS8)時(shí)采集所有兔只的血液,測(cè)定并分析血清生化指標(biāo)及其變化(△HS)情況,并與耐熱時(shí)間進(jìn)行相關(guān)性分析。結(jié)果表明:熱應(yīng)激對(duì)血清葡萄糖(GLU)、甘油三酯(TG)、膽固醇(TC)、總蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)、AST/ALT、堿性磷酸酶(ALP)、磷酸肌酸激酶(CPK)及其變化值均無(wú)顯著影響(P0.05),極顯著影響血清超氧化物歧化酶(SOD)及其變化值(P0.01)。相關(guān)分析表明,HS0時(shí)的GLB及ALP與耐熱時(shí)間分別呈顯著負(fù)相關(guān)(r=-0.362,P0.05)和顯著正相關(guān)(r=0.376,P0.05),SOD的△HS與耐熱時(shí)間呈顯著正相關(guān)(r=0.396,P0.05),而其他指標(biāo)或其變化值與耐熱時(shí)間的相關(guān)性不顯著(P0.05)。本研究推測(cè),GLB、ALP和SOD可能是重要的肉兔耐熱性評(píng)價(jià)候選指標(biāo)。
[Abstract]:The aim of the experiment was to study the relationship between serum biochemical indexes and heat resistance in the early stage of acute heat stress, and to provide a theoretical basis for screening evaluation indexes of heat tolerance in meat rabbits. Fifty-five 4-month-old healthy New Zealand rabbits born in the same period were randomly assigned to two artificial temperature control rooms. The ambient temperature of the control group was (21 鹵1) 鈩，

本文編號(hào)：2430598