【摘要】：MicroRNA在胚胎合子基因轉(zhuǎn)換、胚胎致密化、DNA甲基化和染色質(zhì)重塑等方面發(fā)揮重要作用;精子中的microRNA可通過調(diào)控早期胚胎轉(zhuǎn)錄水平,直接參于胚胎的發(fā)育,并在跨代遺傳中發(fā)揮重要作用。相比體外受精胚胎,體細胞核移植胚胎表現(xiàn)出較低的發(fā)育潛能。目前多認為這是技術(shù)層面不足和異常重編程導致的,而精子攜帶進入卵母細胞的RNA的作用一直被忽視。本試驗以精源性microRNA為切入點,研究了其對早期核移植胚胎發(fā)育的影響。主要研究結(jié)果如下:Bta-miR-202在精子中高表達,并且在受精胚胎原核時期的表達水平高于卵母細胞以及2細胞時期的受精胚胎。本課題組前期研究證明SEPT7是bta-miR-202的一個靶基因,生物信息學方法分析顯示SEPT7在細胞胞質(zhì)分裂中發(fā)揮重要作用;對SEPT7進行蛋白互作分析,結(jié)果提示SEPT7可能與HDAC6相互作用。SEPT7蛋白在類原核期核移植胚胎的表達水平高于原核期體外受精胚;獲得bta-miR-202核移植胚胎的SEPT7表達水平低于對照組,但仍高于體外受精組;獲得bta-miR-202核移植胚胎的α-tubulin乙�；礁哂趯φ战M,但仍低于體外受精組;獲得bta-miR-202核移植胚胎第一次卵裂發(fā)生碎片率低于對照組,但仍高于體外受精組。Bta-miR-202靶向卵源SEPT7,可能通過SEPT7與HDAC6的互作,調(diào)控a-tubulin乙�；綇亩绊懞艘浦才咛サ牡谝淮温蚜�。綜上所述,bta-miR-202靶向SEPT7調(diào)控牛核移植胚胎第一次卵裂。
[Abstract]:MicroRNA plays an important role in embryo zygote gene transformation, embryo densification, DNA methylation and chromatin remodeling. MicroRNA in spermatozoa can be directly involved in the development of embryos by regulating the transcriptional level of early embryos and play an important role in cross-generation inheritance. Compared with IVF embryos, somatic nuclear transfer embryos showed lower developmental potential. At present, it is believed that this is caused by inadequate technology and abnormal reprogramming, while the role of sperm carrying RNA into oocytes has been neglected. The effects of spermatogenic microRNA on the development of early nuclear transfer embryos were studied. The main results were as follows: the expression of Bta-miR-202 was higher in spermatozoa than in oocytes and 2-cell embryos. Our previous studies have proved that SEPT7 is a target gene of bta-miR-202. Bioinformatics analysis shows that SEPT7 plays an important role in cytokinesis. The results of protein interaction analysis of SEPT7 suggest that SEPT7 may interact with HDAC6. The expression level of SEPT7 protein in prokaryotic phase nuclear transfer embryos is higher than that in prokaryote phase in vitro fertilization embryos. The expression of SEPT7 in the bta-miR-202 nuclear transfer embryos was lower than that in the control group, but still higher than that in the IVF group, and the level of 偽-tubulin acetylation in the bta-miR-202 nuclear transfer embryos was higher than that in the control group, but still lower than that in the IVF group. The first cleavage rate of bta-miR-202 nuclear transfer embryos was lower than that of control group, but still higher than that of IVF group. Bta-miR-202 targeted SEPT7, might interact with HDAC6 through SEPT7. Regulation of a-tubulin acetylation level affects the first cleavage of nuclear transfer embryos. In conclusion, bta-miR-202 targeting SEPT7 regulates the first cleavage of bovine nuclear transfer embryos.
【學位授予單位】：西北農(nóng)林科技大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S823

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相關(guān)期刊論文 前1條

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本文編號：2429503