腺胃型傳染性支氣管炎病毒的分離鑒定
發(fā)布時間：2019-02-15 21:03
【摘要】：從河北病雞腫大腺胃中分離到1株病毒(命名為CK/CH/HBHS/07F株),經(jīng)雞胚傳代、血凝特性測定、新城疫病毒(Newcastle disease virus,NDV)干擾試驗、傳染性支氣管炎病毒(Infectious bronchitis virus,IBV)膜蛋白(membrane protein,M)基因序列分析、人工感染試驗等方法對該病毒進(jìn)行了鑒定。該分離毒株感染雞胚的尿囊液沒有直接血凝活性,經(jīng)胰酶處理后可凝集雞紅細(xì)胞,其血凝活性可被IBV陽性血清所抑制;經(jīng)雞胚傳至第3代時,可致死雞胚或出現(xiàn)侏儒胚;序列分析結(jié)果表明,該病毒M基因與18株不同致病型IBV毒株間的核苷酸和氨基酸序列同源性分別為82.8%~94.1%、82.4%~97.4%,與腺胃型毒株CK/CH/LJL/04I同源性最高,該分離株能人工復(fù)制出與自然病例相似的病理變化。初步證實CK/CH/HBHS/07F株為腺胃型IBV。
[Abstract]:A strain of virus (named CK/CH/HBHS/07F strain) was isolated from the stomach of the enlarged gland of Hebei disease chicken. After the passage of chicken embryo, the determination of hemagglutination characteristics, and the interference test of Newcastle disease virus (Newcastle disease virus,NDV), (Infectious bronchitis virus, of infectious bronchitis virus was obtained. IBV) membrane protein (membrane protein,M) gene sequence analysis, artificial infection test and other methods to identify the virus. The hemagglutination activity of allantoic fluid of chicken embryo infected by this isolate strain was not direct hemagglutination activity, and the hemagglutination activity of chicken erythrocyte could be agglutinated after trypsin treatment, and its hemagglutination activity could be inhibited by IBV positive serum, and it could kill chicken embryo or produce dwarf embryo at the third generation of chicken embryo. Sequence analysis showed that the nucleotide and amino acid sequence homology between M gene of the virus and 18 strains of different pathogenic IBV strains were 82.8% and 82.4%, respectively. The CK/CH/LJL/04I strain had the highest homology with the adenogastric virus strain, and the isolated strain could replicate the pathological changes similar to those of the natural cases. Preliminary confirmation of CK/CH/HBHS/07F strain as adenogastric IBV.
【作者單位】： 河北農(nóng)業(yè)大學(xué)動物醫(yī)學(xué)院/農(nóng)業(yè)部動物疫病病原生物學(xué)華北科學(xué)觀測實驗站;石家莊市畜牧水產(chǎn)局;遷安市農(nóng)業(yè)畜牧水產(chǎn)局;河北省畜牧獸醫(yī)研究所;
【基金】：河北省科技計劃項目(17226613D-3) 河北省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系項目(HBCT2013090202)
【分類號】：S852.65
，
本文編號：2423694
[Abstract]:A strain of virus (named CK/CH/HBHS/07F strain) was isolated from the stomach of the enlarged gland of Hebei disease chicken. After the passage of chicken embryo, the determination of hemagglutination characteristics, and the interference test of Newcastle disease virus (Newcastle disease virus,NDV), (Infectious bronchitis virus, of infectious bronchitis virus was obtained. IBV) membrane protein (membrane protein,M) gene sequence analysis, artificial infection test and other methods to identify the virus. The hemagglutination activity of allantoic fluid of chicken embryo infected by this isolate strain was not direct hemagglutination activity, and the hemagglutination activity of chicken erythrocyte could be agglutinated after trypsin treatment, and its hemagglutination activity could be inhibited by IBV positive serum, and it could kill chicken embryo or produce dwarf embryo at the third generation of chicken embryo. Sequence analysis showed that the nucleotide and amino acid sequence homology between M gene of the virus and 18 strains of different pathogenic IBV strains were 82.8% and 82.4%, respectively. The CK/CH/LJL/04I strain had the highest homology with the adenogastric virus strain, and the isolated strain could replicate the pathological changes similar to those of the natural cases. Preliminary confirmation of CK/CH/HBHS/07F strain as adenogastric IBV.
【作者單位】： 河北農(nóng)業(yè)大學(xué)動物醫(yī)學(xué)院/農(nóng)業(yè)部動物疫病病原生物學(xué)華北科學(xué)觀測實驗站;石家莊市畜牧水產(chǎn)局;遷安市農(nóng)業(yè)畜牧水產(chǎn)局;河北省畜牧獸醫(yī)研究所;
【基金】：河北省科技計劃項目(17226613D-3) 河北省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系項目(HBCT2013090202)
【分類號】：S852.65
，
本文編號：2423694
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