茶多酚對(duì)豬精液常溫保存效果的影響
發(fā)布時(shí)間：2019-02-14 21:31
【摘要】：【目的】研究茶多酚(TPP)對(duì)豬精液常溫保存效果的影響�！痉椒ā渴治辗ú杉�20月齡健康杜洛克種公豬精液,在基礎(chǔ)稀釋液中添加不同質(zhì)量濃度的TPP(0(CK),0.01,0.02,0.04,0.08,0.16g/L)作為稀釋液進(jìn)行常溫(17℃)保存,每隔24h檢查精子活率、質(zhì)膜完整率和頂體完整率,分別在精液保存0,2,4,6d時(shí)檢測(cè)精液中的細(xì)菌含量,并在3d和6d時(shí)檢測(cè)精液中的丙二醛含量(MDA)和總抗氧化(T-AOC)活性�！窘Y(jié)果】添加0.02,0.04,0.08g/L TPP可明顯提高豬精子活率、質(zhì)膜完整率、頂體完整率和T-AOC活性,降低精液的MDA含量和細(xì)菌含量。且以0.04g/L TPP效果最佳,精液有效保存時(shí)間為6d。6d時(shí)0.04g/L TPP組豬精子活率、質(zhì)膜完整率、頂體完整率、丙二醛(MDA)含量、總抗氧化(T-AOC)活性和細(xì)菌含量分別為60.02%,46.40%,75.25%,2.39nmol/L,2.31U/mL和2.19×106 CFU/mL�！窘Y(jié)論】茶多酚能有效提高豬精液常溫保存效果,最適添加量為0.04g/L。
[Abstract]:[objective] to study the effect of tea polyphenols (TPP) on the preservation of porcine semen at room temperature. [methods] 20 month-old healthy Duroc boar semen was collected by hand grip method, and TPP (0 (CK),) was added to the basic diluent. The sperm motility rate, plasma membrane integrity rate and acrosome integrity rate were examined every 24 hours, and the bacterial content in semen was measured after 6 days of semen preservation, respectively, at 0.16 g / L 0.08 ~ 0.08 ~ 0.16 g / L as diluent solution, and the sperm motility rate, plasma membrane integrity rate and acrosome integrity rate were examined every 24 hours. The content of malondialdehyde (MDA) and the activity of total antioxidant (T-AOC) in semen were measured at 3 and 6 days. [results] the sperm motility and plasma membrane integrity were significantly increased by adding 0.02n0.04 + 0.08g / L TPP. Acrosome integrity rate and T-AOC activity decreased MDA content and bacterial content in semen. The best effect was 0.04g/L TPP, and the sperm motility, plasma membrane integrity, acrosome integrity, malondialdehyde (MDA) (MDA) content in 0.04g/L TPP group were the best when the effective preservation time of semen was 6d.6d. The total antioxidant activity (T-AOC) activity and bacterial content were 60.02 and 46.400.The total antioxidant activity and bacterial content were 75.25 and 2.39 nmol / L 2.31 U / mL and 2.19 脳 10 ~ 6 CFU/mL., respectively. [conclusion] Tea polyphenols can effectively improve the preservation effect of porcine semen at room temperature. The optimum addition amount is 0.04 g / L.
【作者單位】： 西北農(nóng)林科技大學(xué)動(dòng)物科技學(xué)院;
【基金】：國(guó)家生豬產(chǎn)業(yè)技術(shù)體系項(xiàng)目(CARS-36) 楊凌示范區(qū)科技計(jì)劃項(xiàng)目(2014NY-22) 西北農(nóng)林科技大學(xué)試驗(yàn)示范站(基地)科技成果推廣項(xiàng)目(TGZX2015-18)
【分類號(hào)】：S828
[Abstract]:[objective] to study the effect of tea polyphenols (TPP) on the preservation of porcine semen at room temperature. [methods] 20 month-old healthy Duroc boar semen was collected by hand grip method, and TPP (0 (CK),) was added to the basic diluent. The sperm motility rate, plasma membrane integrity rate and acrosome integrity rate were examined every 24 hours, and the bacterial content in semen was measured after 6 days of semen preservation, respectively, at 0.16 g / L 0.08 ~ 0.08 ~ 0.16 g / L as diluent solution, and the sperm motility rate, plasma membrane integrity rate and acrosome integrity rate were examined every 24 hours. The content of malondialdehyde (MDA) and the activity of total antioxidant (T-AOC) in semen were measured at 3 and 6 days. [results] the sperm motility and plasma membrane integrity were significantly increased by adding 0.02n0.04 + 0.08g / L TPP. Acrosome integrity rate and T-AOC activity decreased MDA content and bacterial content in semen. The best effect was 0.04g/L TPP, and the sperm motility, plasma membrane integrity, acrosome integrity, malondialdehyde (MDA) (MDA) content in 0.04g/L TPP group were the best when the effective preservation time of semen was 6d.6d. The total antioxidant activity (T-AOC) activity and bacterial content were 60.02 and 46.400.The total antioxidant activity and bacterial content were 75.25 and 2.39 nmol / L 2.31 U / mL and 2.19 脳 10 ~ 6 CFU/mL., respectively. [conclusion] Tea polyphenols can effectively improve the preservation effect of porcine semen at room temperature. The optimum addition amount is 0.04 g / L.
【作者單位】： 西北農(nóng)林科技大學(xué)動(dòng)物科技學(xué)院;
【基金】：國(guó)家生豬產(chǎn)業(yè)技術(shù)體系項(xiàng)目(CARS-36) 楊凌示范區(qū)科技計(jì)劃項(xiàng)目(2014NY-22) 西北農(nóng)林科技大學(xué)試驗(yàn)示范站(基地)科技成果推廣項(xiàng)目(TGZX2015-18)
【分類號(hào)】：S828
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