發(fā)布時(shí)間：2019-02-09 15:29

【摘要】：為驗(yàn)證新獸藥白虎定喘口服液的藥效作用,進(jìn)行了雞傳染性支氣管炎病毒(infectious bronchitis virus,IBV)雞胚增殖抑制試驗(yàn)、氣管纖毛運(yùn)動(dòng)試驗(yàn)及SPF雛雞人工感染IBV療效試驗(yàn)。將試驗(yàn)藥液與等量IBV液混合作用后,接種11日齡SPF雞胚,37℃孵育168h,取存活雞胚腎臟進(jìn)行IBV的RT-PCR檢測(cè),結(jié)果表明,藥物組168h雞胚存活率為80%,陽(yáng)性對(duì)照組為20%,兩組間差異顯著(P0.05);試驗(yàn)藥物組RT-PCR檢測(cè)無(wú)目的條帶出現(xiàn),而陽(yáng)性對(duì)照組有目的條帶出現(xiàn)。40日齡SPF雞連續(xù)用藥3d后,氣管內(nèi)注入0.02mL墨汁,測(cè)量1min內(nèi)墨汁移動(dòng)的距離,結(jié)果表明藥物組墨汁移動(dòng)距離極顯著大于生理鹽水對(duì)照組(P0.01)。17日齡SPF雞用IBV-M41滴鼻,0.3mL/只,24h后開始灌服藥液,1mL/kg,連用5d,結(jié)果表明,試驗(yàn)藥物組與攻毒對(duì)照組在臨床癥狀計(jì)分、痊愈率上均存在顯著差異(P0.05);試驗(yàn)組氣管黏膜組織切片未見明顯病變,而陽(yáng)性對(duì)照組有明顯病理變化。以上試驗(yàn)結(jié)果表明,白虎定喘口服液對(duì)IBV具有較好的體外抑制作用,可顯著促進(jìn)氣管纖毛的運(yùn)動(dòng),對(duì)雞傳染性支氣管炎(IB)具有較好的臨床防制效果。
[Abstract]:In order to verify the efficacy of Baihu Dingchuan Oral liquid, a new veterinary drug, chicken embryo proliferation inhibition test, tracheociliary exercise test and SPF chicken artificial infection IBV efficacy test were carried out. After mixed with the same amount of IBV solution, 11 day old SPF chicken embryos were inoculated and incubated at 37 鈩，

本文編號(hào)：2419102