【摘要】：在我國的雞群中普遍存在禽白血病病毒和其它病原的共感染,造成了養(yǎng)禽業(yè)很大的危害和經(jīng)濟(jì)損失。之前的研究主要集中在禽白血病病毒的各個(gè)亞群之間,禽白血病病毒和其它一些病毒之間,很少有ALV-B和細(xì)菌性疾病共感染的文獻(xiàn)報(bào)道。為了探討ALV-B和鼠傷寒沙門氏菌的共感染可能具有致病作用,因此我們做了以下研究。從某雞場(chǎng)病雞的泄殖腔棉拭子中分離出一株菌,對(duì)該菌的半數(shù)致死量、致病性和凝集抗原進(jìn)行研究。將120只健康1日齡SPF雛雞隨機(jī)分成A-D 4個(gè)組,分別接種該菌。每周每組分別隨機(jī)抽取3只,采集法氏囊、胸腺、脾臟稱重,計(jì)算免疫器官指數(shù);觀察臨床癥狀和病理組織學(xué)變化;檢測(cè)p27抗原及ALV-B抗體,血清中INF-γ、IL-2和鼠傷寒沙門氏菌的抗體。根據(jù)測(cè)序結(jié)果并與國內(nèi)外參考株進(jìn)行比較,發(fā)現(xiàn)與Typhimurium strain LT2.seq同源性最高為99.7%,最終確定為鼠傷寒沙門氏菌。鼠傷寒沙門氏菌的半數(shù)致死量為1.52×10~4CFU。鼠傷寒沙門氏菌陽性血清與自制的鼠傷寒沙門氏菌凝集抗原出現(xiàn)凝集現(xiàn)象,與雞白痢沙門氏菌的交叉免疫學(xué)試驗(yàn)沒有出現(xiàn)凝集現(xiàn)象。共感染組死亡率為23.33%,高于鼠傷寒沙門氏菌組(13.3%),ALV-B組(16.67%)。單感染組和共感染組的雞都呈現(xiàn)一定程度生長發(fā)育遲緩的現(xiàn)象,其中,共感染組體重顯著低于對(duì)照組(p0.05),也明顯低于單感染組。鼠傷寒沙門氏菌單感染組脾臟腫大,脾臟指數(shù)升高;ALV-B單感染組和共感染組使脾臟指數(shù)下降,共感染組顯著低于對(duì)照組(p0.05)。各感染組的胸腺和法氏囊免疫器官指數(shù)較對(duì)照組有不同程度的降低,并且共感染組更能加重對(duì)免疫器官的損害。單感染組和共感染組病雞都出現(xiàn)了精神萎靡不振、食欲下降、瘦弱的現(xiàn)象。ALV-B單感染組和共感染組剖檢后發(fā)現(xiàn)肝臟略微腫大,肉眼觀察雞的各臟器,并沒有發(fā)現(xiàn)明顯的病變。鼠傷寒沙門氏菌單感染組剖檢發(fā)現(xiàn)有針尖狀出血,出血性腸炎,肝臟腫大,脾臟腫大。在肝臟內(nèi)炎性細(xì)胞浸潤形成壞死灶,脾臟炎性細(xì)胞浸潤形成壞死灶,淋巴細(xì)胞壞死,腎小管上皮細(xì)胞變性等一系列現(xiàn)象。單感染和共感染組的IL-2和干擾IFN-γ的分泌量與對(duì)照組差異顯著(p0.05),并且共感染時(shí)分泌量更低。檢測(cè)泄殖腔p27發(fā)現(xiàn),第2周ALV-B感染組4.34%排毒;第5周ALV-B感染組50%,共感染組54.45%排毒。前4周未檢測(cè)到ALV-B抗體,ALV-B抑制機(jī)體的細(xì)胞免疫和體液免疫,導(dǎo)致疫病的爆發(fā)。由于1日齡SPF雞感染ALV-B很容易發(fā)生免疫耐受,導(dǎo)致感染雞持續(xù)帶毒排毒而不產(chǎn)生抗體。從檢測(cè)鼠傷寒沙門氏菌抗體結(jié)果顯示,共感染組第5W時(shí)陽性率最高為45.45%,表明共感染對(duì)雞的危害性要比單感染嚴(yán)重。綜上所述,分離得到一株鼠傷寒沙門氏菌,并制備了該菌凝集抗原。建立了ALV-B和鼠傷寒沙門氏菌感染1日齡SPF雛雞的模型,表明ALV-B和鼠傷寒沙門氏菌共感染可能具有協(xié)同致病作用。
[Abstract]:The co-infection of avian leukemia virus and other pathogens is common in chickens in China, which causes great harm and economic loss to poultry industry. Previous studies have focused on avian leukemia virus subsets, avian leukemia virus and some other viruses. There are few reports of co-infection of ALV-B and bacterial diseases. To explore the possible pathogenicity of co-infection of ALV-B and Salmonella typhimurium, we did the following research. A strain of a bacterium was isolated from cloacal cotton swab of a chicken farm and its lethal dose pathogenicity and agglutination antigen were studied. 120 1-day-old SPF chicks were randomly divided into 4 groups and inoculated respectively. Three rats were randomly selected from each group each week to weigh the bursa of Fabricius thymus and spleen to calculate the immune organ index and to observe the changes of clinical symptoms and histopathology. P27 antigen and ALV-B antibody, serum INF- 緯, IL-2 and antibody against Salmonella typhimurium were detected. According to the results of sequencing and comparing with the reference strains at home and abroad, it was found that the highest homology with Typhimurium strain LT2.seq was 99.7%, and it was finally identified as Salmonella typhimurium. The median lethal dose of Salmonella typhimurium was 1.52 脳 10 ~ 4 CFU. The positive serum of Salmonella typhimurium was agglutinated with the self-made antigen of Salmonella typhimurium, and the cross-immunological test with Salmonella pullorum had no agglutination. The mortality of co-infection group was 23.33, higher than that of Salmonella typhimurium group (13.3%) and ALV-B group (16.67%). Both the single infection group and the co-infection group showed a certain degree of growth retardation. The body weight of the co-infected group was significantly lower than that of the control group (p0.05) and significantly lower than that of the single infection group. Splenomegaly and spleen index were increased in single infection group of Salmonella typhimurium, but decreased in single infection group and co-infection group in ALV-B group, which were significantly lower in co-infection group than in control group (p0.05). The immune organ index of thymus and bursa of each infection group was lower than that of control group, and the damage of immune organ was more serious in co-infection group. In the single infection group and co-infection group, the diseased chickens showed mental malaise, loss of appetite and weakness. The liver was slightly enlarged in the ALV-B single infection group and co-infection group, and the organs of the chicken were observed with the naked eye. No obvious lesions were found. In the single infection group of Salmonella typhimurium, needle bleeding, hemorrhagic enteritis, hepatomegaly and splenomegaly were found. In the liver, inflammatory cells infiltrate into necrotic foci, splenic inflammatory cells infiltrate into necrotic foci, lymphocyte necrosis, renal tubular epithelial cell degeneration and a series of phenomena. The secretion of IL-2 and interfering IFN- 緯 in single infection and coinfection group was significantly different from that in control group (p0.05), and the secretion was lower in co-infection group. In the second week, 4.34% of the patients were infected with ALV-B, and 54.45% in the group of ALV-B infection at the 5th week. ALV-B antibodies were not detected in the first 4 weeks, and ALV-B inhibited cellular and humoral immunity, leading to the outbreak of blight. The immune tolerance of 1 day old SPF chickens infected with ALV-B was easy to develop, which led to continuous detoxification without antibody production in infected chickens. The results of detection of Salmonella typhimurium antibody showed that the highest positive rate of co-infection was 45.45 at the 5th week, which indicated that co-infection was more harmful to chicken than to single infection. In conclusion, a strain of Salmonella typhimurium was isolated and its agglutination antigen was prepared. The model of SPF chicks infected with ALV-B and Salmonella typhimurium was established, which indicated that co-infection of ALV-B and Salmonella typhimurium might have synergistic pathogenicity.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.31

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 胡新崗;黃銀云;郭廣富;金彩蓮;;豬鼠傷寒沙門氏菌的分離與鑒定[J];畜牧與獸醫(yī);2005年11期

2 李仲連;;鼠傷寒沙門氏菌對(duì)幼犢感染的試驗(yàn)[J];青海畜牧獸醫(yī);1981年02期

3 畢運(yùn)龍;李春棣;李時(shí)寅;孟開興;楊潤蘭;胡金鳳;;兔暴發(fā)鼠傷寒沙門氏菌病的調(diào)查[J];中國畜禽傳染病;1987年05期

4 李凱年;;用牛奶或乳清降低肉用仔雞鼠傷寒沙門氏菌濃度[J];吉林畜牧獸醫(yī);1991年05期

5 張書存,項(xiàng)玉燕;國外對(duì)鼠傷寒沙門氏菌的研究概況[J];肉品衛(wèi)生;2000年12期

6 劉振湘,鐘福生;小靈貓鼠傷寒沙門氏菌的分離與鑒定[J];中國動(dòng)物檢疫;2001年11期

7 胡家輝;吳祖立;;鴿鼠傷寒沙門氏菌血清凝集方法的建立[J];畜牧市場(chǎng);2009年06期

8 張琦;付明哲;陳曉霖;;豬鼠傷寒沙門氏菌的分離與鑒定[J];中國畜牧獸醫(yī)文摘;2011年04期

9 劉陽;吳白;張竹君;陸倩倩;王彥紅;;一株鵝源性鼠傷寒沙門氏菌的分離與鑒定[J];上海畜牧獸醫(yī)通訊;2013年02期

10 付啟勇;;接種鼠傷寒沙門氏菌小雞的白細(xì)胞游走抑制作用[J];國外獸醫(yī)學(xué).畜禽疾病;1984年03期

相關(guān)會(huì)議論文 前10條

1 官玉容;劉作義;;35株鼠傷寒沙門氏菌藥物敏感及臨床情況分析[A];重慶市預(yù)防醫(yī)學(xué)會(huì)2004年學(xué)術(shù)交流會(huì)論文集[C];2004年

2 董方;曾明;;鼠傷寒沙門氏菌基因敲除的研究進(jìn)展[A];2013年中國藥學(xué)大會(huì)暨第十三屆中國藥師周論文集[C];2013年

3 郭愛珍;Dieter M.Schifferli;;鼠傷寒沙門氏菌1型菌毛介導(dǎo)細(xì)菌對(duì)小鼠樹突狀細(xì)胞的粘附及侵襲研究[A];中國畜牧獸醫(yī)學(xué)會(huì)家畜傳染病學(xué)分會(huì)成立20周年慶典暨第十次學(xué)術(shù)研討會(huì)論文集（下）[C];2003年

4 李鵬成;葉小蘭;王志勝;庾慶華;楊倩;;乳酸桿菌S-層蛋白對(duì)鼠傷寒沙門氏菌黏附及入侵Caco-2細(xì)胞的作用[A];中國畜牧獸醫(yī)學(xué)會(huì)動(dòng)物解剖學(xué)及組織胚胎學(xué)分會(huì)第十六次學(xué)術(shù)研討會(huì)論文集[C];2010年

5 溫馨;郝鳳奇;楊桂連;王春鳳;;鼠傷寒沙門氏菌鞭毛蛋白flic基因的克隆及在乳酸菌中的表達(dá)[A];第四屆第十次全國學(xué)術(shù)研討會(huì)暨動(dòng)物微生態(tài)企業(yè)發(fā)展戰(zhàn)略論壇論文集（下冊(cè)）[C];2010年

6 李正;程相朝;張春杰;張明亮;田芳華;龍塔;;鼠傷寒沙門氏菌crp/cya缺失株的構(gòu)建及其生物學(xué)特性初步研究[A];2012年中國畜牧獸醫(yī)學(xué)會(huì)獸醫(yī)病理學(xué)分會(huì)暨中國病理生理學(xué)會(huì)動(dòng)物病理生理專業(yè)委員會(huì)學(xué)術(shù)研討會(huì)論文集[C];2012年

7 倪學(xué)勤;Joshua Gong;曾東;Weido si;周小秋;;豬源乳酸桿菌對(duì)鼠傷寒沙門氏菌DT104在豬腸道上皮細(xì)胞IPEC-J2上粘附的影響[A];第四屆第九次全國學(xué)術(shù)研討會(huì)暨飼料和動(dòng)物源食品安全戰(zhàn)略論壇論文集（上冊(cè)）[C];2008年

8 朱森林;陳e，

本文編號(hào)：2418717