肉雞腹水綜合征（AS）中MIF與AMPK表達(dá)關(guān)系的研究
發(fā)布時(shí)間：2019-01-30 17:26
【摘要】：[目的]肉雞腹水綜合征(AS)嚴(yán)重影響?zhàn)B雞業(yè)經(jīng)濟(jì)效益和肌肉品質(zhì)。以往主要集中于研究缺氧引起的肺動(dòng)脈高壓環(huán)節(jié),但是不能全面揭示該病的發(fā)病機(jī)制。從病理解剖和病理生理看,該病的顯著特征是右心肥大,心臟是肉雞腹水綜合征的主要靶器官。本文認(rèn)為肉雞腹水綜合征本質(zhì)上屬代謝性疾病,慢性心衰導(dǎo)致肉雞腹水綜合征。AMPK是心肌能量代謝平衡調(diào)節(jié)器,巨噬細(xì)胞移動(dòng)抑制因子(MIF)是影響AMPK活性的重要細(xì)胞因子。因此,本文從肉雞心臟代謝角度入手研究肉雞腹水綜合征。[方法]本研究以臨床上的腹水綜合征肉雞為研究對(duì)象,采用免疫印跡方法檢測(cè)MIF、AMPK、p-AMPK在臨床健康肉雞與腹水綜合征肉雞心臟組織的表達(dá)水平,以探究肉雞腹水綜合征中MIF與AMPK的表達(dá)關(guān)系。[結(jié)果]1采用免疫印跡方法對(duì)MIF在臨床健康肉雞心臟、肝臟、脾臟、肺臟、腎臟的表達(dá)情況進(jìn)行分析,結(jié)果顯示：MIF在各組織中均有表達(dá)。2采用免疫印跡方法對(duì)MIF在臨床健康肉雞與腹水綜合征肉雞中心臟、肝臟、脾臟、肺臟、腎臟的表達(dá)情況進(jìn)行分析,結(jié)果顯示：MIF在腹水綜合征肉雞的心臟、肝臟表達(dá)顯著增加,在腎臟的表達(dá)顯著下降,在脾臟和肺臟的表達(dá)增加,但差異不顯著。3采用免疫印跡方法對(duì)AMPK、p-AMPK在腹水綜合征肉雞心臟中的表達(dá)情況進(jìn)行分析,結(jié)果顯示：腹水綜合征肉雞的MPK磷酸水平顯著升高。[結(jié)論]肉雞腹水綜合征發(fā)生時(shí),心肌MIF顯著增加,AMPK顯著活化,減少心肌損傷,為AMPK信號(hào)通路調(diào)節(jié)AS的心肌能量代謝機(jī)制提供了新思路。
[Abstract]:[objective] (AS) of broiler ascites syndrome seriously affected the economic benefit and muscle quality of chicken industry. In the past, it was mainly focused on the study of hypoxic pulmonary hypertension, but it can not fully reveal the pathogenesis of the disease. According to pathological anatomy and pathophysiology, the disease is characterized by the fat right heart and the heart is the main target organ of ascites syndrome in broilers. It is considered that ascites syndrome in broilers is essentially a metabolic disease, chronic heart failure leads to ascites syndrome in broilers, AMPK is a balance regulator of myocardial energy metabolism, and macrophage migration inhibitor (MIF) is an important cytokine affecting the activity of AMPK. Therefore, this paper studies ascites syndrome in broiler from the angle of heart metabolism. [methods] the expression of MIF,AMPK,p-AMPK in the heart of clinical broilers with ascites syndrome was detected by Western blot. To explore the expression of MIF and AMPK in broiler ascites syndrome. [results] 1the expression of MIF in heart, liver, spleen, lung and kidney of healthy broilers was analyzed by Western blot. The results showed that MIF was expressed in all tissues. 2 the expression of MIF in heart, liver, spleen, lung and kidney of clinical healthy broilers and broilers with ascites syndrome was analyzed by Western blot. The results showed that the expression of MIF in the heart and liver of broilers with ascites syndrome was significantly increased, the expression in kidney was decreased significantly, the expression in spleen and lung was increased, but the difference was not significant. 3 Immunoblot method was used to detect AMPK,. The expression of p-AMPK in the heart of broilers with ascites syndrome was analyzed. The results showed that the level of MPK phosphoric acid in broilers with ascites syndrome was significantly increased. [conclusion] during the occurrence of ascites syndrome in broilers, myocardial MIF was significantly increased, AMPK was activated significantly and myocardial injury was reduced, which provided a new idea for the regulation of myocardial energy metabolism mechanism of AS by AMPK signaling pathway.
【學(xué)位授予單位】：山西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.31
本文編號(hào)：2418346
[Abstract]:[objective] (AS) of broiler ascites syndrome seriously affected the economic benefit and muscle quality of chicken industry. In the past, it was mainly focused on the study of hypoxic pulmonary hypertension, but it can not fully reveal the pathogenesis of the disease. According to pathological anatomy and pathophysiology, the disease is characterized by the fat right heart and the heart is the main target organ of ascites syndrome in broilers. It is considered that ascites syndrome in broilers is essentially a metabolic disease, chronic heart failure leads to ascites syndrome in broilers, AMPK is a balance regulator of myocardial energy metabolism, and macrophage migration inhibitor (MIF) is an important cytokine affecting the activity of AMPK. Therefore, this paper studies ascites syndrome in broiler from the angle of heart metabolism. [methods] the expression of MIF,AMPK,p-AMPK in the heart of clinical broilers with ascites syndrome was detected by Western blot. To explore the expression of MIF and AMPK in broiler ascites syndrome. [results] 1the expression of MIF in heart, liver, spleen, lung and kidney of healthy broilers was analyzed by Western blot. The results showed that MIF was expressed in all tissues. 2 the expression of MIF in heart, liver, spleen, lung and kidney of clinical healthy broilers and broilers with ascites syndrome was analyzed by Western blot. The results showed that the expression of MIF in the heart and liver of broilers with ascites syndrome was significantly increased, the expression in kidney was decreased significantly, the expression in spleen and lung was increased, but the difference was not significant. 3 Immunoblot method was used to detect AMPK,. The expression of p-AMPK in the heart of broilers with ascites syndrome was analyzed. The results showed that the level of MPK phosphoric acid in broilers with ascites syndrome was significantly increased. [conclusion] during the occurrence of ascites syndrome in broilers, myocardial MIF was significantly increased, AMPK was activated significantly and myocardial injury was reduced, which provided a new idea for the regulation of myocardial energy metabolism mechanism of AS by AMPK signaling pathway.
【學(xué)位授予單位】：山西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.31
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