肉雞腹水綜合征（AS）中MIF與AMPK表達關(guān)系的研究
發(fā)布時間：2019-01-30 17:26
【摘要】：[目的]肉雞腹水綜合征(AS)嚴重影響?zhàn)B雞業(yè)經(jīng)濟效益和肌肉品質(zhì)。以往主要集中于研究缺氧引起的肺動脈高壓環(huán)節(jié),但是不能全面揭示該病的發(fā)病機制。從病理解剖和病理生理看,該病的顯著特征是右心肥大,心臟是肉雞腹水綜合征的主要靶器官。本文認為肉雞腹水綜合征本質(zhì)上屬代謝性疾病,慢性心衰導致肉雞腹水綜合征。AMPK是心肌能量代謝平衡調(diào)節(jié)器,巨噬細胞移動抑制因子(MIF)是影響AMPK活性的重要細胞因子。因此,本文從肉雞心臟代謝角度入手研究肉雞腹水綜合征。[方法]本研究以臨床上的腹水綜合征肉雞為研究對象,采用免疫印跡方法檢測MIF、AMPK、p-AMPK在臨床健康肉雞與腹水綜合征肉雞心臟組織的表達水平,以探究肉雞腹水綜合征中MIF與AMPK的表達關(guān)系。[結(jié)果]1采用免疫印跡方法對MIF在臨床健康肉雞心臟、肝臟、脾臟、肺臟、腎臟的表達情況進行分析,結(jié)果顯示：MIF在各組織中均有表達。2采用免疫印跡方法對MIF在臨床健康肉雞與腹水綜合征肉雞中心臟、肝臟、脾臟、肺臟、腎臟的表達情況進行分析,結(jié)果顯示：MIF在腹水綜合征肉雞的心臟、肝臟表達顯著增加,在腎臟的表達顯著下降,在脾臟和肺臟的表達增加,但差異不顯著。3采用免疫印跡方法對AMPK、p-AMPK在腹水綜合征肉雞心臟中的表達情況進行分析,結(jié)果顯示：腹水綜合征肉雞的MPK磷酸水平顯著升高。[結(jié)論]肉雞腹水綜合征發(fā)生時,心肌MIF顯著增加,AMPK顯著活化,減少心肌損傷,為AMPK信號通路調(diào)節(jié)AS的心肌能量代謝機制提供了新思路。
[Abstract]:[objective] (AS) of broiler ascites syndrome seriously affected the economic benefit and muscle quality of chicken industry. In the past, it was mainly focused on the study of hypoxic pulmonary hypertension, but it can not fully reveal the pathogenesis of the disease. According to pathological anatomy and pathophysiology, the disease is characterized by the fat right heart and the heart is the main target organ of ascites syndrome in broilers. It is considered that ascites syndrome in broilers is essentially a metabolic disease, chronic heart failure leads to ascites syndrome in broilers, AMPK is a balance regulator of myocardial energy metabolism, and macrophage migration inhibitor (MIF) is an important cytokine affecting the activity of AMPK. Therefore, this paper studies ascites syndrome in broiler from the angle of heart metabolism. [methods] the expression of MIF,AMPK,p-AMPK in the heart of clinical broilers with ascites syndrome was detected by Western blot. To explore the expression of MIF and AMPK in broiler ascites syndrome. [results] 1the expression of MIF in heart, liver, spleen, lung and kidney of healthy broilers was analyzed by Western blot. The results showed that MIF was expressed in all tissues. 2 the expression of MIF in heart, liver, spleen, lung and kidney of clinical healthy broilers and broilers with ascites syndrome was analyzed by Western blot. The results showed that the expression of MIF in the heart and liver of broilers with ascites syndrome was significantly increased, the expression in kidney was decreased significantly, the expression in spleen and lung was increased, but the difference was not significant. 3 Immunoblot method was used to detect AMPK,. The expression of p-AMPK in the heart of broilers with ascites syndrome was analyzed. The results showed that the level of MPK phosphoric acid in broilers with ascites syndrome was significantly increased. [conclusion] during the occurrence of ascites syndrome in broilers, myocardial MIF was significantly increased, AMPK was activated significantly and myocardial injury was reduced, which provided a new idea for the regulation of myocardial energy metabolism mechanism of AS by AMPK signaling pathway.
【學位授予單位】：山西農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S858.31
本文編號：2418346
[Abstract]:[objective] (AS) of broiler ascites syndrome seriously affected the economic benefit and muscle quality of chicken industry. In the past, it was mainly focused on the study of hypoxic pulmonary hypertension, but it can not fully reveal the pathogenesis of the disease. According to pathological anatomy and pathophysiology, the disease is characterized by the fat right heart and the heart is the main target organ of ascites syndrome in broilers. It is considered that ascites syndrome in broilers is essentially a metabolic disease, chronic heart failure leads to ascites syndrome in broilers, AMPK is a balance regulator of myocardial energy metabolism, and macrophage migration inhibitor (MIF) is an important cytokine affecting the activity of AMPK. Therefore, this paper studies ascites syndrome in broiler from the angle of heart metabolism. [methods] the expression of MIF,AMPK,p-AMPK in the heart of clinical broilers with ascites syndrome was detected by Western blot. To explore the expression of MIF and AMPK in broiler ascites syndrome. [results] 1the expression of MIF in heart, liver, spleen, lung and kidney of healthy broilers was analyzed by Western blot. The results showed that MIF was expressed in all tissues. 2 the expression of MIF in heart, liver, spleen, lung and kidney of clinical healthy broilers and broilers with ascites syndrome was analyzed by Western blot. The results showed that the expression of MIF in the heart and liver of broilers with ascites syndrome was significantly increased, the expression in kidney was decreased significantly, the expression in spleen and lung was increased, but the difference was not significant. 3 Immunoblot method was used to detect AMPK,. The expression of p-AMPK in the heart of broilers with ascites syndrome was analyzed. The results showed that the level of MPK phosphoric acid in broilers with ascites syndrome was significantly increased. [conclusion] during the occurrence of ascites syndrome in broilers, myocardial MIF was significantly increased, AMPK was activated significantly and myocardial injury was reduced, which provided a new idea for the regulation of myocardial energy metabolism mechanism of AS by AMPK signaling pathway.
【學位授予單位】：山西農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S858.31
【參考文獻】
相關(guān)期刊論文 前2條
1 李彩東;楊勇衛(wèi);李惠軍;田鵬飛;吳斌;;慢性HBV攜帶者外周血巨噬細胞移動抑制因子、白細胞介素10和17水平的變化及其與臨床指標的相關(guān)性[J];臨床肝膽病雜志;2015年05期
2 沈佳佳;黃瑛;祁煒罡;談晶花;張代富;;白藜蘆醇通過促進自噬減輕糖尿病大鼠心肌缺血再灌注損傷的實驗研究[J];中國臨床醫(yī)學;2015年02期
相關(guān)博士學位論文 前1條
1 張昱;PJ-34對心肌缺血再灌注損傷的保護作用及其抗氧化應(yīng)激損傷的機制研究[D];浙江大學;2011年
相關(guān)碩士學位論文 前1條
1 李冬冬;脂聯(lián)素通過AdipoR1-APPL1信號通路保護缺血/再灌注心肌[D];第四軍醫(yī)大學;2012年
，本文編號：2418346
本文鏈接：http://sikaile.net/yixuelunwen/dongwuyixue/2418346.html
最近更新
教材專著
