【摘要】：探討郁金散對大腸濕熱證模型大鼠血清和腸道組織胃腸激素的影響。Wistar大鼠60只,體重180~220g,隨機分為正常對照組、大腸濕熱證模型組、自愈組、郁金散高、中、低劑量治療組,每組10只。通過自由飲用蜂蜜水;前10d禁食與飼喂充足飼料并灌胃豬油隔日交替進行;之后5d每天灌白酒,并置于高溫高濕環(huán)境(8h·d-1);然后腹腔注射大腸桿菌,24h后再注射一次建立大腸濕熱證大鼠模型。造模成功后,分別給予高(10.4g·kg~(-1))、中(5.2g·kg~(-1))、低(2.6g·kg~(-1))劑量郁金散進行治療。整個試驗過程中觀察大鼠的臨床癥狀和體征,記錄體重變化,檢測血清和回腸、結腸組織中的胃腸激素含量。結果顯示:模型大鼠出現(xiàn)便溏、糞便色黃惡臭、肛門紅腫、發(fā)熱等癥狀和體征。正常組體重逐漸上升,模型大鼠造模期間體重呈現(xiàn)先波狀上升、再逐漸下降的趨勢,郁金散治療后體重呈逐漸上升的趨勢,其中以高劑量組回升最明顯,但各組均無顯著性差異(P0.05)。與正常對照組相比,模型組和自愈組大鼠血清、回腸及結腸組織中胃動素(MTL)、胃泌素(GAS)、P物質(SP)含量極顯著或顯著升高(P0.01或P0.05),血管活性腸肽(VIP)無顯著變化,生長抑素(SS)含量極顯著降低(P0.01);與自愈組相比,各劑量郁金散治療后,MTL、GAS、SP、VIP和SS都有不同程度的回調,以高劑量組回調最為明顯,其中高劑量組大鼠血清、回腸、結腸中MTL、GAS和SP極顯著或顯著降低(P0.01或P0.05),SS極顯著或顯著升高(P0.01或P0.05)。大腸濕熱證模型大鼠機體出現(xiàn)了胃腸激素紊亂,表現(xiàn)為MTL、GAS、SP和VIP含量升高,而SS含量降低,郁金散能改善大腸濕熱證模型大鼠機體胃腸激素異常水平,且高劑量的治療效果最好。
[Abstract]:To explore the effect of Yujin Powder on gastrointestinal hormones in serum and intestinal tissue of rats with large intestine damp-heat syndrome. Sixty Wistar rats, weighing 180g / 220 g, were randomly divided into three groups: normal control group, model group of large intestine damp-heat syndrome, self-healing group, high, middle and low dose treatment group. 10 rats in each group. After drinking honey water freely, fasting for the first 10 days, feeding enough feed and gavage lard alternately, after 5 days, liquor was infused daily and placed in high temperature and high humidity environment (8h 路d ~ (-1). Then the coliform was injected intraperitoneally, and the rat model of large intestine damp-heat syndrome was established again after 24 hours. After the model was successfully established, the high (10.4 g kg~ (-1),) (5.2 g kg~ (-1),) low dose (2.6 g kg~ (-1) of Yujin Powder) was given respectively. The clinical symptoms and signs of the rats were observed, the changes of body weight were recorded, and the contents of gastrointestinal hormones in serum, ileum and colon were detected. The results showed that the model rats had symptoms and signs, such as loose stools, yellow stench of faeces, red and swollen anus, fever and so on. The body weight of the normal group gradually increased, and the weight of the model rats increased at first, then decreased gradually, and the weight increased gradually after the treatment of Yujin Powder, especially in the high dose group. But there was no significant difference in each group (P0.05). Compared with normal control group, the content of motilin (MTL), gastrin (GAS), P (SP) in serum, ileum and colon of model group and self-healing group was significantly higher than that of normal control group (P0.01 or P0.05). There was no significant change in vasoactive intestinal peptide (VIP), but the content of somatostatin (SS) was significantly decreased (P0.01). Compared with the self-healing group, MTL,GAS,SP,VIP and SS had different degrees of callback, especially in the high-dose group, in which the MTL, in serum, ileum and colon of rats in the high-dose group was more obvious than that in the self-healing group. GAS and SP were significantly decreased (P0.01 or P0.05), SS significantly or significantly increased (P0.01 or P0.05). Gastrointestinal hormone disorder appeared in rats with large intestine damp-heat syndrome, which showed that the contents of MTL,GAS,SP and VIP increased, while the content of SS decreased. Yujin Powder could improve the abnormal level of gastrointestinal hormones in rats with large intestine damp-heat syndrome. And the high dose of the treatment is the best.
【作者單位】： 甘肅農(nóng)業(yè)大學動物醫(yī)學院;
【基金】：國家自然科學基金(31472234) 國家肉牛/牦牛產(chǎn)業(yè)技術體系(GARS-38) 公益性行業(yè)(農(nóng)業(yè))科技專項(201403051)
【分類號】：S853.74
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本文編號：2414152