【摘要】：對神農(nóng)架保護(hù)區(qū)大龍?zhí)侗Ｗo(hù)站1只半野生病死川金絲猴的不同部位的腸道內(nèi)容物進(jìn)行了微生物多樣性研究。在無菌條件下取十二指腸段、小腸段、盲腸段及大腸段4個(gè)部位的腸內(nèi)容物,提取微生物基因組DNA,應(yīng)用高通量測序技術(shù),對細(xì)菌和真菌多樣性進(jìn)行了詳細(xì)的分析,比較了不同部位腸道的細(xì)菌和真菌的豐度、分布、多樣性及變化趨勢等。發(fā)現(xiàn)該川金絲猴腸道細(xì)菌主要分屬于厚壁菌門(Firmicutes)、疣微菌門(Verrucomicrobia)、變形菌門(Proteobacteria)、梭桿菌門(Fusobacteria)、放線菌門(Actinobacteria)、擬桿菌門(Bacteroidetes)和Synergistetes 7個(gè)門,真菌主要分屬于子囊菌門(Ascomycota)、擔(dān)子菌門(Basidiomycota)、接合菌門(Zygomycota)以及未分類真菌(Fungi_unclassified)。細(xì)菌豐度最高的是盲腸段,細(xì)菌多樣性最高的是大腸段,真菌豐度最高的是小腸段,真菌多樣性最高的是大腸段。
[Abstract]:The microbial diversity of intestinal contents in different parts of a semi-wild dead Monkey was studied in Dalong Lake Protection Station of Shennongjia Reserve. The microbial genomic DNA, was extracted from the intestinal contents of duodenum, small intestine, cecum and large intestine in aseptic condition. The diversity of bacteria and fungi was analyzed in detail by high-throughput sequencing technique. The abundance, distribution, diversity and change trend of bacteria and fungi in different parts of intestine were compared. It was found that the intestinal bacteria of the golden monkey mainly belong to 7 phylum of (Actinobacteria), (Bacteroidetes) and Synergistetes, which belong to the phylum of (Firmicutes), (Verrucomicrobia), (Proteobacteria), (Fusobacteria), actinomycetes, and Synergistetes, respectively. Fungi mainly belong to (Basidiomycota), conjugate (Zygomycota) and unclassified fungi (Fungi_unclassified) of ascomycetes (Ascomycota), basidiomycetes. The highest bacterial abundance was in the cecum segment, the highest in bacterial diversity was in the large intestine segment, the highest in the fungal abundance was in the small intestine segment, and the highest in the fungal diversity was in the large intestine segment.
【作者單位】： 北京動物園;圈養(yǎng)野生動物技術(shù)北京市重點(diǎn)實(shí)驗(yàn)室;國家林業(yè)局林產(chǎn)工業(yè)規(guī)劃設(shè)計(jì)院;湖北省金絲猴保護(hù)研究中心;
【基金】：北京市公園管理中心科研項(xiàng)目:高通量測序技術(shù)分析北京動物園大熊貓腸道菌群多樣性,項(xiàng)目編號:zx2015013 國家科技支撐計(jì)劃課題(2013BAD03B01)
【分類號】：S858.93
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本文編號：2409000