【摘要】：為鑒定禽源多殺性巴氏桿菌(Avian Pasteurella multocida,Pm)敏感株與環(huán)丙沙星(Cip)耐藥株的差異表達基因,本研究采用高通量轉錄組測序的RNA-seq技術對敏感株與Cip耐藥株進行檢測,參考Pm70株基因組(NC_002663.1)進行拼接與質量評估,測序數(shù)據(jù)處理后獲得表達差異基因,利用GO和KEGG數(shù)據(jù)庫對表達差異基因進行注釋與富集性分析,并利用熒光定量PCR對轉錄組測序中的差異表達基因進行驗證。結果顯示,所有樣品測序數(shù)據(jù)參考Pm70全基因組匹配率均達到95.87%以上,對基因差異表達進行分析后鑒定得到19個差異表達基因,其中15個上調,4個下調。通過GO功能富集分析顯示差異表達基因主要為轉運運輸功能,并對差異表達基因進行信號通路富集性分析,結果表明最具代表性的信號通路為"ABC轉運蛋白"(ko02010)。推測禽源Pm中的ABC轉運蛋白超家族可能在Cip耐藥機制中具有重要作用。
[Abstract]:In order to identify the differentially expressed genes between susceptible strains of Pasteurella multocida (Avian Pasteurella multocida,Pm) and ciprofloxacin resistant strains (Cip), high throughput transcriptional sequencing (RNA-seq) technique was used to detect sensitive strains and Cip resistant strains. Pm70 genome (NC_002663.1) was used for splicing and quality evaluation. After sequencing data were processed, the differentially expressed genes were obtained. The GO and KEGG databases were used to annotate the differentially expressed genes and to analyze the enrichment of the differentially expressed genes. Fluorescent quantitative PCR was used to verify the differentially expressed genes in transcriptome sequencing. The results showed that the total genome matching rate of all samples was over 95.87%, and 19 differentially expressed genes were identified by analysis of gene differential expression, of which 15 were up-regulated and 4 down-regulated. GO functional enrichment analysis showed that the differentially expressed genes were mainly transporter, and the signal pathway enrichment of differentially expressed genes was analyzed. The results showed that the most representative signal pathway was "ABC transporter" (ko02010). It is suggested that the ABC transporter superfamily in avian Pm may play an important role in the mechanism of Cip resistance.
【作者單位】： 安徽農(nóng)業(yè)大學動物科技學院;湖北省農(nóng)業(yè)科學院畜牧獸醫(yī)研究所;
【基金】：公益性農(nóng)業(yè)行業(yè)科研專項:家禽主要細菌病防控技術研究與示范(201303044) 現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術體系建設專項資金資助(CARS-42-G11) 規(guī)�；B(yǎng)雞場主要疫病防控關鍵技術研究(2015ABA039)
【分類號】：S852.61

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