【摘要】：為研究廣譜性殺菌劑苯并烯氟菌唑通過(guò)飼料在動(dòng)物源產(chǎn)品中的積累和殘留風(fēng)險(xiǎn),采用Qu ECh ERS前處理方法與高效液相色譜-串聯(lián)質(zhì)譜(HPLC MS/MS)相結(jié)合,建立了苯并烯氟菌唑在4種動(dòng)物源產(chǎn)品(豬肉、雞肉、牛肉和雞蛋)中的檢測(cè)方法。樣品用乙腈振蕩提取,經(jīng)N-丙基乙二胺(PSA)分散固相萃取凈化后,HPLC MS/MS檢測(cè),外標(biāo)法定量。結(jié)果表明:在0.001~1 mg/L范圍內(nèi),4種動(dòng)物源產(chǎn)品中苯并烯氟菌唑的色譜峰面積與其相應(yīng)的質(zhì)量濃度間呈良好的線性關(guān)系,r0.991 0。在0.004、0.01和0.1 mg/kg 3個(gè)添加水平下,苯并烯氟菌唑在雞蛋、雞肉、豬肉和牛肉中的回收率分別為83%~90%,82%~96%,85%~96%和77%~97%,相對(duì)標(biāo)準(zhǔn)偏差(RSD)分別為6.9%~11%,5.4%~8.7%,2.6%~7.3%和7.2%~10%。以最小添加水平作為方法的定量限,苯并烯氟菌唑在4種產(chǎn)品中的定量限均為0.004 mg/kg。所建方法具有簡(jiǎn)單、快速、靈敏、高通量等特點(diǎn),適用于動(dòng)物源產(chǎn)品中苯并烯氟菌唑的殘留檢測(cè)。在全國(guó)10個(gè)省份抽取120個(gè)樣品(雞蛋、雞肉、豬肉和牛肉各30個(gè))進(jìn)行了監(jiān)測(cè),均未檢出苯并烯氟菌唑殘留。
[Abstract]:In order to study the accumulation and residual risk of benzofuronazole, a broad-spectrum bactericide, in animal products through feed, the Qu ECh ERS pretreatment method was combined with high performance liquid chromatography-tandem mass spectrometry (HPLC MS/MS). A method for the detection of benzenefluronazole in four animal products (pork, chicken, beef and eggs) was established. The samples were extracted by oscillating acetonitrile, purified by N-propylethylenediamine (PSA) dispersion solid phase extraction, detected by HPLC MS/MS and quantified by external standard method. The results showed that in the range of 0.001 mg/L, there was a good linear relationship between the chromatographic peak area and the corresponding mass concentration of benzofuronazole in the four animal products, r 0.991 0. The recoveries of benzofuronazole in eggs, chicken, pork and beef at the levels of 0. 004 and 0. 1 mg/kg were 83%, 90% and 82% respectively, and the recoveries of benzofuronazole in eggs, chicken, pork and beef were 85%, 96% and 77 75%, respectively. The relative standard deviation (RSD) was 6.9 and 5.4, and about 2.6 percent and 7.2 percent and 10 percent respectively. Using the minimum addition level as the quantitative limit of the method, the quantitative limit of Benzofuronazole in the four products was 0.004 mg/kg.. The method is simple, rapid, sensitive and high throughput. It is suitable for the determination of benzofuronazole residues in animal products. A total of 120 samples (30 eggs, 30 chicken, 30 pork and 30 beef) were collected in 10 provinces of China, and no benzofuronazole residues were detected.
【作者單位】： 中國(guó)農(nóng)業(yè)科學(xué)院煙草研究所;
【分類號(hào)】：S859.84

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