【摘要】：為測定實驗室前期構(gòu)建的可分泌Melittin(1-13)-LL(17-30)(M-L)雜合肽的畢赤酵母工程菌發(fā)酵上清液的抑菌譜以及生物穩(wěn)定性。采用牛津杯瓊脂擴散法測定其對16株指示菌的抑菌效果,以及經(jīng)過模擬胃腸液和不同溫度處理后的穩(wěn)定性。結(jié)果表明,上清液對多種致病菌具有良好的抑菌效果,且抑菌直徑半數(shù)在20mm以上,而對有益菌屎腸球菌無抑菌作用;經(jīng)模擬胃液處理后,活性明顯下降;但經(jīng)過模擬腸液以及不同溫度梯度處理后抑菌活性無明顯變化。因此,雜合肽M-L發(fā)酵液抑菌譜廣,溫度耐受性好,但在胃液中穩(wěn)定性較差。
[Abstract]:In order to determine the bacteriostatic spectrum and biological stability of the fermentation supernatant of Pichia pastoris which secreted Melittin (1-13) -LL (17-30) (M-L) hybrid peptide in early laboratory. Oxford cup Agar diffusion method was used to determine the bacteriostasis effect on 16 strains of indicator bacteria and the stability after simulated gastrointestinal fluid and different temperature treatment. The results showed that the supernatant had a good bacteriostatic effect on a variety of pathogenic bacteria, and the inhibitory diameter of the supernatant was more than half of 20mm, but had no bacteriostatic effect on the beneficial bacteria Enterococcus faecium, and the activity of the supernatant decreased obviously after the treatment of simulated gastric juice. But after simulated intestinal fluid and different temperature gradient treatment, there was no obvious change in bacteriostatic activity. Therefore, the fermentation broth of hybrid peptide M-L has wide antibacterial spectrum and good temperature tolerance, but its stability in gastric juice is poor.
【作者單位】： 中國農(nóng)業(yè)大學(xué)動物科技學(xué)院/動物營養(yǎng)學(xué)國家重點實驗室;
【基金】：國家自然科學(xué)基金資助項目(31272476,31572442) 高等學(xué)校博士學(xué)科點專項科研基金資助(20110008110002)
【分類號】：S816.3

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