【摘要】：為了研究伊犁馬催乳素(PRL)基因、垂體轉(zhuǎn)錄因子(POU1F1)基因多態(tài)及其與產(chǎn)奶量之間的關(guān)系,試驗(yàn)以新疆伊犁昭蘇牧場(chǎng)73頭伊犁馬母馬為研究對(duì)象,利用PCR-RFLP技術(shù)分析了伊犁馬PRL、POU1F1基因序列多態(tài)性,并與日均產(chǎn)奶量進(jìn)行關(guān)聯(lián)分析。結(jié)果顯示,伊犁馬PRL基因第二外顯子上存在1個(gè)SNP:c.210CT;POU1F1基因CDS區(qū)上沒(méi)有發(fā)現(xiàn)多態(tài),但內(nèi)含子區(qū)存在1個(gè)SNP:g.2758GA。POU1F1基因不同基因型間日均母馬產(chǎn)奶量差異不顯著(P0.05),但PRL基因c.210位點(diǎn)多態(tài)對(duì)伊犁馬日均產(chǎn)奶量有顯著影響(P0.05)。因此,PRL基因的多態(tài)位點(diǎn)有潛力用作伊犁馬產(chǎn)奶量性狀選育的分子標(biāo)記。
[Abstract]:In order to study the polymorphism of prolactin (PRL) gene, pituitary transcription factor (POU1F1) gene and milk yield in Yili horse, 73 female Yili horses in Zhaosu pasture, Xinjiang, were studied. The polymorphism of PRL,POU1F1 gene in Yili horse was analyzed by PCR-RFLP technique, and the correlation between PRL,POU1F1 gene polymorphism and daily milk yield was analyzed. The results showed that there was a SNP:c.210CT; on the second exon of PRL gene in Yili horse. No polymorphism was found in the CDS region of POU1F1 gene, but there was no significant difference in milk yield among different genotypes of SNP:g.2758GA.POU1F1 gene (P0.05). But the polymorphism of PRL gene c. 210 had a significant effect on the average daily milk yield of Yili horse (P0.05). Therefore, the polymorphic loci of PRL gene have the potential to be used as molecular markers for the breeding of milk yield traits in Yili horse.
【作者單位】： 西北農(nóng)林科技大學(xué)動(dòng)物科技學(xué)院;新疆農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)學(xué)院;
【基金】：陜西省引進(jìn)人才資助(Z111021202)
【分類號(hào)】：S821.83

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