【摘要】：通過對(duì)體外法(仿生消化法)測(cè)定的玉米的酶水解物能值(EHGE)與體內(nèi)法(動(dòng)物試驗(yàn)法)測(cè)定的真代謝能(TME)進(jìn)行比較,篩選快速、準(zhǔn)確估測(cè)飼料能值的方法。體內(nèi)法采用動(dòng)物試驗(yàn)法進(jìn)行代謝試驗(yàn)測(cè)定TME,選用504只21日齡AA肉仔雞,按體質(zhì)量均分為7個(gè)處理,每個(gè)處理12個(gè)重復(fù),每個(gè)重復(fù)6只雞,其中6個(gè)處理分別飼喂6個(gè)地區(qū)玉米飼料原料,另外1個(gè)處理為饑餓組,測(cè)定其內(nèi)源能量代謝,試驗(yàn)期3 d。體外法采用仿生消化法進(jìn)行仿生消化試驗(yàn)測(cè)定EHGE,6個(gè)地區(qū)玉米飼料原料設(shè)為6個(gè)處理,每個(gè)處理10個(gè)重復(fù),每個(gè)重復(fù)2根消化管。結(jié)果表明:體外法測(cè)定的EHGE和體內(nèi)法測(cè)定的TME總體上差異不顯著。體外法測(cè)定的EHGE的變異系數(shù)在0.21%~0.87%,而體內(nèi)法測(cè)定的TEM的變異系數(shù)在0.98%~4.12%,體外法測(cè)定的EHGE對(duì)體內(nèi)法測(cè)定的TME的估計(jì)偏差均在2%以內(nèi),體外法測(cè)定的EHGE與體內(nèi)法測(cè)定的TME呈顯著線性關(guān)系(R2=0.95)�？梢�,用體外法測(cè)定的EHGE具有較高的準(zhǔn)確性,且達(dá)到了估測(cè)體內(nèi)法TME的要求,能快速、準(zhǔn)確地評(píng)價(jià)日糧的代謝能。
[Abstract]:The enzyme hydrolytic energy (EHGE) of maize determined by bionic digestion in vitro was compared with the true metabolizable energy (TME) measured by in vivo method (animal test) to select a fast and accurate method for estimating feed energy value. In vivo Metabolism Test by Animal Test TME, 504 21-day-old AA broilers were divided into 7 treatments, 12 replicates per treatment, 6 chickens per replica, according to body mass. Among them, 6 treatments were fed with corn feed raw materials in 6 regions respectively, and the other one was treated with starvation group. The endogenous energy metabolism was measured for 3 days. In vitro bionic digestion test was used to determine the corn feed raw materials in 6 areas of EHGE, were treated with 6 treatments with 10 repeats each with 2 digestive tubes per repeat. The results showed that there was no significant difference between in vitro EHGE and in vivo TME. The coefficient of variation of EHGE measured by in vitro method was 0.21 and 0.87, while the coefficient of variation of TEM measured by in vivo method was 0.98 and 4.12.The estimated deviation of EHGE determined by in vitro method to TME determined by in vivo method was within 2%. There was a significant linear relationship between EHGE determined in vitro and TME measured in vivo (R _ 2O _ (0.95). It can be seen that the determination of EHGE by in vitro method has high accuracy and meets the requirement of estimating TME in vivo, which can quickly and accurately evaluate the metabolic energy of diet.
【作者單位】： 河南農(nóng)業(yè)大學(xué)牧醫(yī)工程學(xué)院;河南省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所;
【基金】：國(guó)家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系項(xiàng)目(CARS-42) 河南省農(nóng)業(yè)科學(xué)院自主創(chuàng)新專項(xiàng)
【分類號(hào)】：S816

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