【摘要】：銅是動物機體必需的金屬元素,參與重要的代謝反應。本試驗旨在研究無銅飼料對小鼠腸道微生物菌落組成和相關代謝通路的影響,選擇健康、體重相近、遺傳背景為野生型C57BL/6J的成年公鼠20只,隨機分為2組,試驗組和對照組各10只。待小鼠適應性飼養(yǎng)1周后用無銅飼料飼喂,對照組飼喂25 mg/L含銅水,試驗組飼喂無銅水,試驗期30 d。試驗期結束時,尾靜脈采血進行銅藍蛋白含量的測定,并采集小鼠新鮮盲腸內(nèi)容物利用高通量測序技術對小鼠的盲腸內(nèi)容物16S rRNA基因組進行分析。結果顯示,與對照組相比,無銅組小鼠血清銅藍蛋白含量極顯著降低(P0.001)。無銅組小鼠盲腸微生物菌群在門、屬水平分別與對照組有5和43個差異顯著菌群(P0.05);通過差異菌群功能預測分析發(fā)現(xiàn),兩組共有19種KEGG二級代謝通路差異顯著(P0.05),其中與代謝相關的功能是脂代謝、甘氨酸生物合成與代謝、多酮類化合物和萜類化合物代謝、其他次生代謝產(chǎn)物的生物合成和氨基酸代謝,說明飼料中銅的缺失可以改變少數(shù)腸道菌群的組成及其功能,本試驗為研究銅在小鼠腸道菌群結構及代謝中的功能提供了重要思路。
[Abstract]:Copper is an essential metal element in animal body and is involved in important metabolic reactions. The purpose of this study was to study the effects of copper free diet on the composition and related metabolic pathways of intestinal microbial colonies in mice. Twenty adult male rats with similar body weight and genetic background of wild type C57BL/6J were selected and randomly divided into 2 groups. There were 10 rats in the test group and 10 in the control group. The mice were fed with copper-free feed for 1 week, the control group was fed with copper-containing water for 25 mg/L, and the experimental group was fed with copper-free water for 30 days. At the end of the trial period, the caudal vein blood samples were collected for the determination of ceruloplasmin content, and the fresh caecum contents of mice were collected. The 16s rRNA genomes of the caecum contents were analyzed by high-throughput sequencing technique. The results showed that compared with the control group, the serum ceruloplasmin content in the copper free group was significantly lower than that in the control group (P0.001). There were 5 and 43 significant differences in the cecum microflora between the copper free group and the control group (P0.05). It was found that there were 19 KEGG secondary metabolic pathways in the two groups (P0.05), among which lipid metabolism, glycine biosynthesis and metabolism were related to metabolism. Metabolism of polyketones, terpenoids, other secondary metabolites, biosynthesis and amino acid metabolism suggests that the absence of copper in feed can change the composition and function of a few intestinal flora. This experiment provides an important way to study the function of copper in intestinal microflora structure and metabolism of mice.
【作者單位】： 中國農(nóng)業(yè)科學院北京畜牧獸醫(yī)研究所;
【基金】：中國農(nóng)業(yè)科學院基本科研業(yè)務費預算增量項目(2015ywf-zd-30) 中國農(nóng)業(yè)科學院北京畜牧獸醫(yī)研究所科技創(chuàng)新工程特設項目(ASTIP-IAS-TS-02)
【分類號】：S816

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