國內(nèi)豬源卡他莫拉菌的首次分離與鑒定
發(fā)布時(shí)間：2018-12-18 11:49
【摘要】：2015年1月昆明市郊區(qū)某規(guī)�；N豬場(chǎng)飼養(yǎng)的后備種豬發(fā)生一種以高熱,呼吸急促、窘迫,皮膚發(fā)紺及猝死為特征的疫情。分離細(xì)菌培養(yǎng)后獲得1株形態(tài)呈雙腎形的革蘭氏陰性雙球菌。經(jīng)昆明小白鼠致病性實(shí)驗(yàn)、本動(dòng)物回歸實(shí)驗(yàn)、細(xì)菌生化鑒定及16S r RNA和Usp A1基因序列分析顯示,該分離菌株為毒力極強(qiáng)的卡他莫拉菌。與卡他莫拉菌參考菌株BBH18株(Gen Bank登錄號(hào):NC_014147.1)的16S r RNA和Usp A1基因序列同源性達(dá)100%。藥敏實(shí)驗(yàn)結(jié)果顯示,該菌株對(duì)頭孢噻肟高度敏感,對(duì)妥布霉素、慶大霉素、頭孢曲松、頭孢呋辛、頭孢他啶、呋喃妥因及四環(huán)素中度敏感。豬場(chǎng)選用敏感藥物進(jìn)行緊急預(yù)防和治療,及時(shí)有效地控制了本病的擴(kuò)散及蔓延。
[Abstract]:In January 2015, an epidemic situation characterized by high fever, shortness of breath, distress, cyanosis and sudden death occurred in a large scale pig farm in Kunming suburb. A strain of Gram-negative bicoccus with double kidney shape was obtained after culture. The pathogenicity test of Kunming mice, the animal regression test, the biochemical identification of bacteria and the sequence analysis of 16s r RNA and Usp A1 gene showed that the strain was highly virulent. The sequence homology of 16s r RNA and Usp A1 gene with reference strain BBH18 strain (Gen Bank accession number: NC_014147.1) was 100%. The results showed that the strain was highly sensitive to cefotaxime and moderately sensitive to tobramycin, gentamicin, ceftriaxone, cefuroxime, ceftazidime, furantoin and tetracycline. The use of sensitive drugs in pig farms for emergency prevention and treatment, timely and effective control of the spread and spread of the disease.
【作者單位】： 云南省貢山縣農(nóng)業(yè)和科學(xué)技術(shù)局;云南省巍山縣動(dòng)物疫病預(yù)防與控制中心;云南省墨江縣動(dòng)物疫病預(yù)防與控制中心;云南省富民縣動(dòng)物疫病預(yù)防與控制中心;云南農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院;云南省畜牧獸醫(yī)科學(xué)院
【基金】：云南省科技廳重大科技專項(xiàng)(2012ZA017)
【分類號(hào)】：S852.61
本文編號(hào)：2385806
[Abstract]:In January 2015, an epidemic situation characterized by high fever, shortness of breath, distress, cyanosis and sudden death occurred in a large scale pig farm in Kunming suburb. A strain of Gram-negative bicoccus with double kidney shape was obtained after culture. The pathogenicity test of Kunming mice, the animal regression test, the biochemical identification of bacteria and the sequence analysis of 16s r RNA and Usp A1 gene showed that the strain was highly virulent. The sequence homology of 16s r RNA and Usp A1 gene with reference strain BBH18 strain (Gen Bank accession number: NC_014147.1) was 100%. The results showed that the strain was highly sensitive to cefotaxime and moderately sensitive to tobramycin, gentamicin, ceftriaxone, cefuroxime, ceftazidime, furantoin and tetracycline. The use of sensitive drugs in pig farms for emergency prevention and treatment, timely and effective control of the spread and spread of the disease.
【作者單位】： 云南省貢山縣農(nóng)業(yè)和科學(xué)技術(shù)局;云南省巍山縣動(dòng)物疫病預(yù)防與控制中心;云南省墨江縣動(dòng)物疫病預(yù)防與控制中心;云南省富民縣動(dòng)物疫病預(yù)防與控制中心;云南農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院;云南省畜牧獸醫(yī)科學(xué)院
【基金】：云南省科技廳重大科技專項(xiàng)(2012ZA017)
【分類號(hào)】：S852.61
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