【摘要】：為了預測奶牛常用飼料中可利用氨基酸(u AA)的含量,本試驗采集了21個奶牛飼料原料樣品,測定了飼料樣品的粗蛋白質(CP)、粗脂肪(EE)、粗灰分(ash)、中性洗滌纖維(NDF)、酸性洗滌纖維(ADF)、可溶性粗蛋白質(SCP)、非蛋白氮(NPN)、中性洗滌劑不溶粗蛋白質(NDIP)、酸性洗滌劑不溶粗蛋白質(ADIP)含量,并根據(jù)康奈爾凈碳水化合物-蛋白質體系(CNCPS)對飼料含氮化合物組分的劃分方法,計算出飼料樣品的含氮化合物組分非蛋白氮(PA)、快速降解真蛋白質(PB1)、中速降解真蛋白質(PB2)、慢速降解真蛋白質(PB3)和不可利用蛋白質(PC)的含量,測定了飼料的氨基酸含量,同時利用飼料氨基酸含量與u AA含量之間的關系估測出飼料的u AA含量。結果表明,奶牛常用飼料的CNCPS含氮化合物組分(PA、PB1、PB2、PB3、PC)含量(%DM)與u AA、可利用蛋氨酸(utilizable methionine,u Met)、可利用賴氨酸(utilizable lysine,u Lys)、可利用亮氨酸(utilizable leucine,u Leu)含量(%DM)存在顯著的相關關系,回歸方程如下:u AA=0.171PA+0.134PB1+0.364PB2+0.370PB3+0.148PC+7.785,R2=0.99,P0.01;u M et=0.019PA-0.031PB1+0.024PB2+0.078PB3-0.004PC-0.071,R2=0.93,P0.01;u Lys=-0.045PA-0.020PB1+0.072PB2+0.038PB3-0.011PC+0.780,R2=0.92,P0.01;u Leu=0.071PA+0.033PB1+0.093PB2+0.189PB3+0.003PC+0.006,R2=0.94,P0.01。結果顯示,根據(jù)CNCPS含氮化合物組分和本試驗所建立的方程,可以準確地預測奶牛飼料的u AA、u M et、u Lys和u Leu含量。
[Abstract]:In order to predict the content of available amino acid (u AA) in the common feed of dairy cattle, 21 samples of feed raw materials were collected and the crude protein (CP), crude fat (EE), crude ash (ash), of feed sample was determined in this experiment. Neutral detergent fiber (NDF), acid detergent (ADF), soluble crude protein (SCP), non-protein nitrogen (NPN), neutral detergent insoluble crude protein (NDIP), acid detergent insoluble crude protein (ADIP) content, According to the method of dividing nitrogenous compounds in feed by Cornell net carbohydrate protein system (CNCPS), the fast degradation of true protein (PB1) by non-protein nitrogen component (PA), of feed sample was calculated. The contents of PB2, PB3 and (PC) were determined. At the same time, the content of u AA in feed was estimated by the relationship between the content of amino acid and the content of u AA. The results showed that the content of CNCPS nitrogen-containing compounds (PA,PB1,PB2,PB3,PC) (% DM) and the use of methionine (utilizable methionine,u Met),) (utilizable lysine,u Lys),) by u AA, were available in dairy cattle feed. There was a significant correlation between the content of leucine (utilizable leucine,u Leu) (% DM) and the regression equation as follows: U AA=0.171PA 0.134PB1 0.364PB2 0.370PB3 0.148PC 7.785 R2N 0.99G P0.01; UM et=0.019PA-0.031PB1 0.024PB2 0.078PB3-0.004PC-0.071P0.01U Lys=-0.045PA-0.020PB1 0.072PB2 0.038PB3-0.011PC 0.780 R20.92 (P0.01); U Leu=0.071PA 0.033PB1 0.093PB2 0.189PB3 0.003PC 0.006, R2O0.94, P0.01. The results showed that the content of u AA,u M et,u Lys and u Leu in dairy feed could be accurately predicted according to the composition of nitrogen compounds in CNCPS and the equation established in this experiment.
【作者單位】： 中國農業(yè)大學動物科技學院;
【基金】：基金項目:現(xiàn)代農業(yè)產業(yè)技術體系北京市創(chuàng)新團隊
【分類號】：S823.5
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本文編號：2376272