【摘要】：本文研究了不同劑量的中藥復(fù)方添加劑“曲枳散”對斷奶仔豬生長性能及主要免疫指標(biāo)的影響,探索“曲枳散”的最佳添加劑量及其作用機(jī)理,旨在開發(fā)一種高效、綠色的中藥飼料添加劑。仔豬飼喂添加試驗(yàn):試驗(yàn)選用21日齡斷奶仔豬48頭,隨機(jī)分為4個處理,每個處理12頭,分別飼喂基礎(chǔ)日糧、基礎(chǔ)日糧+1%中藥復(fù)方、基礎(chǔ)日糧+3%中藥復(fù)方、基礎(chǔ)日糧+6%的中藥復(fù)方,相對應(yīng)的分別為對照組、低劑量組、中劑量組和高劑量組。本論文主要包括三部分內(nèi)容:1.中藥復(fù)方“曲枳散”對仔豬生長性能及消化吸收能力的影響。統(tǒng)計仔豬各時期的平均日采食量和平均日增重,計算料重比比值。檢測不同組仔豬主要消化酶活性和仔豬回腸絨毛長度、隱窩深度。試驗(yàn)結(jié)果表明,添加3%劑量的中劑量組仔豬平均日采食量和平均日增重均顯著高于對照組(P0.05),仔豬料重比比值顯著小于對照組(P0.05)。中劑量組仔豬胃蛋白酶、蔗糖酶和麥芽糖酶活性顯著高于對照組(P0.05),同時中藥復(fù)方能夠增加回腸絨毛長度和隱窩深度,從而提高仔豬對營養(yǎng)物質(zhì)消化吸收能力,提高仔豬的生產(chǎn)性能。2.中藥復(fù)方“曲枳散”對仔豬血液理化指標(biāo)及主要組織器官的影響。通過對不同組仔豬飼喂添加不同劑量的中藥復(fù)方,檢測仔豬血常規(guī)、血生化指標(biāo)和對主要組織器官切片進(jìn)行觀察,結(jié)果顯示,同對照組比較,在飼喂前期,中劑量組仔豬白細(xì)胞數(shù)目顯著高于對照組(P0.05),因此有利于仔豬抵抗外界病原微生物。各組仔豬血常規(guī)及血生化指標(biāo)均在正常范圍內(nèi),仔豬主要組織切片觀察無明顯病變,因此在仔豬日糧中添加1%~6%的中藥復(fù)方對仔豬無毒性作用。3.中藥復(fù)方“曲枳散”對仔豬抗氧化及部分免疫指標(biāo)的影響。檢測仔豬血清及回腸勻漿中SOD、GSH-Px和MDA含量,3%劑量的中藥復(fù)方能夠顯著增強(qiáng)仔豬抗氧化能力。檢測仔豬血清和回腸勻漿中IgG、血清溶菌酶含量,不同劑量的中藥復(fù)方能夠不同程度提高仔豬血清、回腸勻漿中IgG和血清中溶菌酶含量,中劑量組仔豬血清和回腸勻漿中Ig G、血清中溶菌酶含量顯著高于對照組(P0.05),因此3%的中藥復(fù)方能夠顯著增強(qiáng)仔豬的免疫力。同時,對仔豬回腸免疫相關(guān)細(xì)胞因子mRNA和仔豬外周血T淋巴細(xì)胞亞群檢測,由檢測結(jié)果可知,中藥復(fù)方能夠通過提高IL-2,TGF-β含量,提高仔豬CD3+CD4+、CD3+CD8+T淋巴細(xì)胞比例和仔豬外周血CD3+CD4+/CD3+CD8+比值,從而增強(qiáng)仔豬的免疫平衡。
[Abstract]:In this paper, the effects of different dosages of traditional Chinese medicine compound additive "qu Zhi San" on growth performance and main immune indexes of weaned piglets were studied, and the optimum dosage and mechanism of "qu Zhi San" were explored in order to develop a high efficiency. Green Chinese medicine feed additive. Feeding experiment of piglets: 48 piglets of 21 days old weaned piglets were randomly divided into 4 treatments, 12 piglets treated with each treatment, fed with basal diet, 1% Chinese medicine compound diet, 3% Chinese medicine compound diet, and 3% traditional Chinese medicine compound diet respectively. The basic diet of 6% Chinese medicine compound, corresponding to the control group, low dose group, middle dose group and high dose group. This paper mainly includes three parts: 1. Effect of traditional Chinese Medicine compound Quzhi Powder on growth performance and digestibility of piglets. The average daily intake and average daily gain of piglets were calculated and the ratio of feed to weight was calculated. The main digestive enzyme activity, villus length and crypt depth of piglets were measured. The results showed that the average daily intake and average daily gain of piglets in the medium dose group were significantly higher than those in the control group (P0.05), and the ration of feed to weight ratio of piglets was significantly lower than that of the control group (P0.05). The activities of pepsin, sucrase and maltase in the middle dose group were significantly higher than those in the control group (P0.05). Improving the performance of piglets. 2. Effects of traditional Chinese Medicine compound Quzhi Powder on Blood Physicochemical Indexes and main tissues and organs of piglets. By feeding different groups of piglets with different dosages of traditional Chinese medicine compound, the blood routine, biochemical indexes of blood and the sections of main tissues and organs were observed. The results showed that, compared with the control group, the piglets were treated in the early stage of feeding. The number of white blood cells in the middle dose group was significantly higher than that in the control group (P0.05), which was beneficial to the resistance of piglets to external pathogenic microorganisms. The blood routine and biochemical indexes of piglets in each group were in the normal range, and no obvious changes were observed in the main tissue sections of piglets, so adding 1% compound Chinese medicine to the diet of piglets had no toxic effect on piglets. 3. Effects of traditional Chinese Medicine compound Quzhi Powder on Antioxidant and partial immune Indexes of piglets. The contents of SOD,GSH-Px and MDA in serum and ileum homogenate of piglets were determined. The antioxidant ability of piglets was significantly enhanced by 3% Chinese medicine compound. The levels of IgG, lysozyme in serum and ileum homogenate of piglets were detected. Different doses of Chinese herbal medicine compound could increase the contents of IgG and lysozyme in serum, ileum homogenate, and Ig G in serum and ileum homogenate of piglets. The content of lysozyme in serum was significantly higher than that in control group (P0.05), so 3% Chinese herbal compound could significantly enhance the immunity of piglets. At the same time, mRNA and T lymphocyte subsets in peripheral blood of piglets were detected. The results showed that the traditional Chinese medicine compound could improve the CD3 CD4 of piglets by increasing the content of IL-2,TGF- 尾. The ratio of CD3 CD8 T lymphocytes and the ratio of CD3 CD4 / CD3 CD8 in peripheral blood of piglets increased the immune balance of piglets.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S828.5

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