【摘要】：雞白痢是由雞沙門氏菌引起的一種發(fā)生極為普遍、危害極為嚴(yán)重的傳染病。也是影響雞養(yǎng)殖業(yè)健康發(fā)展的重要疾病之一,發(fā)生雞白痢病造成的經(jīng)濟(jì)損失遠(yuǎn)大于新城疫,禽流感等重大動(dòng)物疫病。因此,我們對新鄉(xiāng)地區(qū)的雞白痢沙門氏菌進(jìn)行了流行病學(xué)調(diào)查、分離培養(yǎng)和生化鑒定、敏感藥物篩選以及聯(lián)合用藥等試驗(yàn),旨在研制出對新鄉(xiāng)地區(qū)雞白痢沙門氏菌敏感的復(fù)方藥物,從而為雞白痢沙門氏菌的防控提供一味良藥。1.新鄉(xiāng)地區(qū)雞白痢沙門氏菌病流行病學(xué)調(diào)查為了 了解新鄉(xiāng)地區(qū)雞白痢沙門氏菌的流行情況,我們采用了常規(guī)的流行病學(xué)調(diào)查方法和沙門氏菌全血玻片凝集法對新鄉(xiāng)地區(qū)40個(gè)養(yǎng)殖場的400份血液進(jìn)行了檢測。結(jié)果發(fā)現(xiàn),新鄉(xiāng)地區(qū)個(gè)別雞場存在沙門氏菌感染的情況,且從400份采集血樣中檢出58份疑似沙門氏菌感染血液,發(fā)病率大約為15%,雞沙門氏菌病呈零星分布。2.雞白痢沙門氏菌的分離培養(yǎng)和鑒定為了確定疑似病雞是否為沙門氏菌中雞白痢沙門氏菌,我們對疑似的58只進(jìn)行了病料采集、細(xì)菌分離培養(yǎng)、生化鑒定和血清學(xué)鑒定。最后從58份沙門氏菌病料中分離鑒定出22份雞白痢沙門氏菌菌株。3.雞白痢沙門氏菌敏感性藥物篩選為了能夠篩選出對新鄉(xiāng)地區(qū)雞白痢沙門氏菌敏感的藥物,本試驗(yàn)采用藥敏紙片擴(kuò)散法對22株沙門氏菌進(jìn)行了敏感藥物篩選。通過篩選發(fā)現(xiàn):新鄉(xiāng)地區(qū)的雞白痢沙門氏菌對氟喹諾酮類藥物和多肽類藥物中的硫酸粘桿菌素為中度敏感,而對鏈霉素、阿莫西林、氟苯尼考、乙酰甲喹等藥物低度敏感或不敏感。4.新鄉(xiāng)地區(qū)雞白痢沙門氏菌聯(lián)合用藥措施為了防控新鄉(xiāng)地區(qū)雞白痢沙門氏菌病。本試驗(yàn)將對新鄉(xiāng)地區(qū)雞白痢沙門氏菌敏感的氟喹諾酮類藥物中的恩諾沙星和多肽類中的硫酸粘桿菌素進(jìn)來復(fù)方配制,并通過對雞白痢沙門氏菌體外和體內(nèi)殺菌試驗(yàn),我們發(fā)現(xiàn)復(fù)方恩諾沙星-硫酸粘桿菌素比單獨(dú)使用恩諾沙星或硫酸粘桿菌的體外殺菌試驗(yàn)提高了 8倍或16倍;在體內(nèi)試驗(yàn)上提高了30%或 40%
[Abstract]:White dysentery is an infectious disease caused by salmonella. It is also one of the important diseases that affect the healthy development of chicken breeding. The economic loss caused by white dysentery is much greater than that caused by Newcastle disease, avian flu and other major animal diseases. Therefore, we conducted epidemiological investigation, isolation, culture and biochemical identification, screening of sensitive drugs and combined use of drugs for salmonella pullorum in Xinxiang area. The aim is to develop a compound drug sensitive to Salmonella pullorum in Xinxiang area, so as to provide a good medicine for the prevention and control of Salmonella pullorum. Epidemiological investigation of Salmonella pullorum in chickens in Xinxiang area in order to understand the prevalence of Salmonella pullorum in Xinxiang area, We used routine epidemiological investigation method and salmonella whole blood slide agglutination method to detect 400 blood samples from 40 farms in Xinxiang area. The results showed that there was salmonella infection in individual chicken farms in Xinxiang area, and 58 samples of suspected Salmonella infection were detected from 400 blood samples. The incidence of Salmonella infection was about 15. Isolation, Culture and Identification of Salmonella pullorum in order to determine whether the suspected infected chicken is Salmonella pullorum, 58 suspected Salmonella pullorum were collected, isolated, and identified by biochemical and serological methods. Finally, 22 strains of Salmonella pullorum were isolated from 58 samples of Salmonella pullorum. Screening of Salmonella pullorum sensitive drugs in order to screen the sensitive drugs to Salmonella pullorum in Xinxiang area 22 strains of Salmonella pullorum were screened by drug sensitive disk diffusion method. It was found that Salmonella pullorum in Xinxiang area was moderately sensitive to myxomycin sulfate in fluoroquinolones and polypeptide drugs, but to streptomycin, amoxicillin and florfenicol. Acetylmethaquine and other drugs are low sensitivity or insensitive. 4. Combined use of Salmonella pullorum in Xinxiang area to prevent and control Salmonella pullorum in Xinxiang area. In this experiment, enrofloxacin in salmonella pullorum sensitive drugs and mucin sulfate in polypeptides were prepared in Xinxiang area, and the bactericidal test of Salmonella pullorum in vitro and in vivo was carried out. We found that the in vitro bactericidal test of compound enrofloxacin-myxobacterium sulfate was 8 or 16 times higher than that of enrofloxacin or myxobacterium sulfate alone. Increased by 30% or 40% in vivo.
【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.31

【引證文獻(xiàn)】

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1 廖潔丹;劉敏芳;譚華龍;陳建紅;張濟(jì)培;;竹絲雞雞白痢沙門菌的分離鑒定與藥敏試驗(yàn)[J];中國家禽;2017年16期

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本文編號：2368789