【摘要】：羊偽結(jié)核病(ovine pseudotuberculosis)又稱羊干酪樣淋巴結(jié)炎(caseous lymphadenitis),是由偽結(jié)核棒狀桿菌(Corynebacterium pseudotuberculosis)所引發(fā)的人畜共患慢性病,綿羊和山羊多發(fā),可造成患病羊消瘦、生產(chǎn)性能低下和產(chǎn)死胎,對養(yǎng)羊業(yè)的危害較大。石泉縣位于陜西省安康市以西,北依秦嶺、南枕巴山,非常適合陜南白山羊養(yǎng)殖,一直以來是安康市陜南白山山羊養(yǎng)殖大縣之一,僅2014年出欄陜南白山羊64119只。近年來石泉縣養(yǎng)羊戶反映所養(yǎng)的陜南白山羊群中有部分羊只在前肢上部出現(xiàn)一種“膿包”,多種方法治療效果不佳,在羊群中持續(xù)存在,造成了較大的經(jīng)濟損失。本文對該病進行了發(fā)病情況調(diào)查、診斷和防治試驗,獲得了以下結(jié)果。(1)臨床發(fā)病調(diào)查結(jié)果表明,隨機抽取的飼養(yǎng)量在50只以上的圈養(yǎng)和散養(yǎng)大戶各10個,圈養(yǎng)公羊、母羊和羔羊“膿包”平均發(fā)病率分別為46.4%、38.1%和1.5%,散養(yǎng)公羊、母羊和羔羊“膿包”平均發(fā)病率分別為69.6%、59.1%和2.9%。(2)從隨機采取的患病羊只病料30份中分離培養(yǎng)到1株疑似偽結(jié)核棒狀桿菌。應(yīng)用細菌16S rRNA通用引物進行PCR擴增和序列分析表明,該分離菌與羊偽結(jié)核棒狀桿菌16S rRNA序列的同源性為99.5%,確定分離菌株為羊偽結(jié)核棒狀桿菌,確診該病為羊偽結(jié)核病。(3)藥敏試驗結(jié)果表明,該菌對環(huán)丙沙星、頭孢曲松、紅霉素、卡那霉素、頭孢噻肟、妥布霉素、強力霉素、慶大霉素、氯霉素等抗菌藥物高度敏感;對四環(huán)素、利福平、鏈霉素等中度敏感。臨床治療試驗結(jié)果表明,利福平和頭孢噻肟鈉經(jīng)過皮下注射給藥方式,對羊偽結(jié)核病治療效果較好。(4)應(yīng)用分離到的羊偽結(jié)核棒狀桿菌研制了滅活鋁膠疫苗,在安全性試驗和無菌試驗證明疫苗正常的情況下,進行小規(guī)模田間試驗的結(jié)果表明,在一年的時間里,接種疫苗的30只羊中只有1只羊患上了羊偽結(jié)核病,發(fā)病率在3.3%,保護率為96.7%,而注射無菌生理鹽水的對照組30只羊中有23只羊患上了羊偽結(jié)核病,發(fā)病率為76.7%。說明研制的疫苗對山羊偽結(jié)核病有很好的預(yù)防效果。
[Abstract]:Sheep pseudotuberculosis (ovine pseudotuberculosis), also called goat caseous lymphadenitis (caseous lymphadenitis), is a chronic zoonotic disease caused by Corynebacterium pseudotuberculosis (Corynebacterium pseudotuberculosis). Sheep and goats have multiple diseases, which can lead to emaciation, low productivity and stillbirth in sheep. The harm to sheep industry is great. Shiquan County is located in the west of Ankang City, Shaanxi Province, north according to the Qinling Mountains, Nanxingba Mountain, very suitable for the breeding of white goats in southern Shaanxi, has been one of the largest counties of Ankang City, Shannan White Mountain goat breeding, only in 2014, 64119 white goats in southern Shaanxi. In recent years, Sheep farmers in Shiquan County have reported that some of the sheep in the Southern Shaanxi White Goat herd have a kind of "abscess" in the upper part of the forelimb. The following results were obtained from the investigation, diagnosis and prevention and treatment of the disease. (1) the results of the clinical investigation showed that 10 large captive sheep and 10 free-range large households were randomly selected, and the male sheep were kept in captivity. The average incidence of abscess in ewe and lamb was 38.1% and 1.5%, respectively. The average incidence of sheep, ewe and lamb was 69.6%, respectively. 59.1% and 2.9% respectively. (2) A suspected pseudotuberculosis corynebacterium was isolated from 30 samples of diseased sheep. The results of PCR amplification and sequence analysis using 16s rRNA universal primers showed that the homology of 16s rRNA sequence between this isolate and Corynebacterium tuberculosis was 99.5, and the isolated strain was identified as Corynebacterium pseudotuberculosis. (3) the drug sensitivity test showed that the strain was resistant to ciprofloxacin, ceftriaxone, erythromycin, kanamycin, cefotaxime, tobramycin, doxycycline, gentamicin, Chloramphenicol and other antimicrobial agents are highly sensitive; Moderately sensitive to tetracycline, rifampicin, streptomycin, etc. The results of clinical treatment showed that rifampicin and cefotaxime sodium were injected subcutaneously to treat pseudo-tuberculosis in sheep. (4) inactivated aluminum gel vaccine was developed by using isolated Corynebacterium pseudotuberculosis in sheep. When the safety test and aseptic test proved that the vaccine was normal, the results of small-scale field trials showed that only one sheep out of 30 vaccinated sheep developed pseudo-tuberculosis in one year, and the incidence rate was 3.3%. The protective rate was 96. 7%, while 23 of the 30 sheep in the control group were infected with pseudotuberculosis, the incidence rate was 76. 7%. The results showed that the vaccine had a good preventive effect on goat pseudopulmonary tuberculosis.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：S858.27

【參考文獻】

相關(guān)期刊論文 前10條

1 李文楊;劉遠;張曉佩;高承芳;陳鑫珠;董曉寧;;福清山羊偽結(jié)核棒狀桿菌病的診治[J];中國畜牧獸醫(yī)文摘;2014年11期

2 李文楊;劉遠;張曉佩;高承芳;陳鑫珠;董曉寧;;山羊偽結(jié)核棒狀桿菌的分離鑒定[J];中國草食動物科學(xué);2014年05期

3 侯萬天;劉界強;;羊偽結(jié)核病的防治[J];畜牧與飼料科學(xué);2014年09期

4 喬宏興;索江華;邊傳周;任敏;;雞傳染性鼻炎氫氧化鋁佐劑滅活苗的制備及免疫試驗[J];動物醫(yī)學(xué)進展;2014年06期

5 曾澤;韋雷飛;黃秀君;張斌;于學(xué)輝;湯承;;一起山羊偽結(jié)核棒狀桿菌感染的診斷[J];動物醫(yī)學(xué)進展;2014年03期

6 廖雄;文正常;王璇;潘淑惠;;山羊偽結(jié)核病的診斷與防治[J];中國畜禽種業(yè);2013年08期

7 范大鵬;;羊偽結(jié)核病的診療與防治措施[J];養(yǎng)殖技術(shù)顧問;2013年06期

8 蘭宇;李一經(jīng);唐麗杰;葛俊偉;王瑞，

本文編號：2367033