發(fā)布時(shí)間：2018-11-26 20:50

【摘要】：本試驗(yàn)旨在調(diào)查北京地區(qū)仔豬腹瀉的原因及豬源大腸桿菌血清型、毒力因子的分布情況。收集北京京郊地區(qū)仔豬腹瀉樣本400份,應(yīng)用含2%血清的TSA培養(yǎng)基分離大腸桿菌,通過血清型鑒定試驗(yàn)和毒力因子檢測驗(yàn)證O抗原。結(jié)果顯示,400份樣本中分離到64株大腸桿菌,定型42株。其主要為O101型(18.7%)、O64型(12.5%)、O8型(10.9%)、O20型(10.9%)、O45型(4.7%)、O149型(4.7%)、O2型(1.6%)、O89型(1.6%)8種血清型,其攜帶的毒力因子為STa、Stx2e、astA和eaeA等。結(jié)果表明,引起京郊仔豬腹瀉的大腸桿菌主要有8個(gè)血清型,其中O101型發(fā)病比例最高。其攜帶的毒力因子主要為astA和eaeA,占全部菌株的50%以上,STa和Stx2e因子占全部菌株的30%以上。本試驗(yàn)結(jié)果將為今后京郊地區(qū)仔豬腹瀉病的防控提供有效的數(shù)據(jù)支持。
[Abstract]:The aim of this study was to investigate the cause of piglet diarrhea and the distribution of serotype and virulence factor of Escherichia coli in Beijing area. A total of 400 samples of piglets with diarrhea were collected from Beijing suburb of Beijing. Escherichia coli was isolated by TSA medium containing 2% serum. O antigen was verified by serotype identification test and virulence factor test. The results showed that 64 strains of Escherichia coli were isolated from 400 samples and 42 strains were identified. O101 (18.7%), O64 (12.5%), O8 (10.9%), O20 (10.9%), O45 (4.7%), O149 (4.7%), O2 (1.6%), The virulence factors of O 89 (1.6%) were STa,Stx2e,astA and eaeA. The results showed that there were mainly 8 serotypes of Escherichia coli causing diarrhea of piglets in Jingjiao, among which O101 had the highest incidence. AstA and eaeA, accounted for more than 50% of the total strains, and STa and Stx2e accounted for more than 30% of the total strains. The results of this study will provide effective data for the prevention and control of piglet diarrhea in Beijing suburb.
【作者單位】： 北京農(nóng)業(yè)職業(yè)學(xué)院;中國人民解放軍軍事醫(yī)學(xué)科學(xué)院;內(nèi)蒙古農(nóng)業(yè)大學(xué);
【基金】：北京市“菜籃子”科技能力提升工程項(xiàng)目 北京農(nóng)業(yè)職業(yè)學(xué)院院級(jí)項(xiàng)目
【分類號(hào)】：S858.28

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 卿柳庭,袁宗輝;鏈球菌毒力因子對(duì)機(jī)體免疫反應(yīng)的影響[J];遼寧畜牧獸醫(yī);2000年01期

2 吳秀芬,李鵬,楊煥民;豬胸膜肺炎放線桿菌毒力因子研究進(jìn)展[J];現(xiàn)代畜牧獸醫(yī);2005年06期

3 范紅結(jié);陸承平;;馬鏈球菌獸疫亞種毒力因子[J];中國人獸共患病學(xué)報(bào);2006年03期

4 曹三杰;段麗麗;肖馳;文心田;;豬2型鏈球菌毒力因子研究進(jìn)展[J];中國人獸共患病學(xué)報(bào);2007年01期

5 楊建德;劉燕霏;;豬胸膜肺炎放線桿菌毒力因子的研究進(jìn)展[J];黑龍江畜牧獸醫(yī);2009年03期

6 陳志寶;姜游帥;夏禮杰;于錄;邱家章;宋科技;卜仕金;鄧旭明;;蜂膠對(duì)金黃色葡萄球菌毒力因子的抑制作用[J];中國獸醫(yī)雜志;2010年11期

7 霍永;李R，

本文編號(hào)：2359588