【摘要】：研究目的：硫磺負離子添加劑是將存在于大自然的多種礦物質(zhì)用現(xiàn)代高科技納米技術(shù)進行粉碎、篩選、分離、烘干等程序加工,并按合理的科學配比混合而成,可廣泛使用于雞鴨、豬狗、牛羊等家畜飼養(yǎng)及魚、蝦等水產(chǎn)類養(yǎng)殖,也可使用于蔬菜、瓜果類的種植和栽培。本實驗主要是通過研究硫磺負離子添加劑對SD大鼠和胎仔致畸敏感期的毒性,為本產(chǎn)品的進一步開發(fā)利用提供一定的數(shù)據(jù)基礎。材料和方法：本實驗選用健康的SD雌性大鼠,在發(fā)情期或發(fā)情間期與雄鼠交配,在雌鼠受孕后的5-15d(胎兒發(fā)育過程中對受試物最敏感的時期)灌胃10mg/kg、33.3mg/kg、100mg/kg的硫磺負離子添加劑混合液,對照組為1%CMC溶液,至受孕第20d處死動物,檢測母體體重、攝食量,觀察孕鼠的臨床癥狀及剖檢后有無內(nèi)臟病變的情況；檢查胎盤重量、黃體數(shù)、著床率、活胎率、胎兒的重量、體長、內(nèi)臟、骨骼發(fā)育情況、雌雄比等,對硫磺負離子添加劑的致畸敏感期生殖毒性進行綜合評價。結(jié)果：SD雌性大鼠口服硫磺負離子添加劑混合液10mg/kg、33.3mg/kg、 100mg/kg后,對受孕SD雌鼠的攝食量,體重、黃體數(shù)、著床率、活胎率；胎仔體重、體長、尾長、肛長,胎仔性別比,胎兒內(nèi)臟及骨骼檢查等各項檢測指標同對照組比較差異無統(tǒng)計學意義(P0.05),與SD大鼠致畸敏感期毒性實驗基數(shù)比較無明顯差異,母體無明顯毒性反應,胎兒也沒出現(xiàn)明顯的致畸作用。結(jié)論：硫磺負離子添加劑在本實驗所用劑量時無毒性和致畸作用,對母鼠的健康及胎鼠的正常發(fā)育均無明顯毒性和致畸作用。
[Abstract]:Objective: sulfur anion additive is a kind of mineral which exists in nature and is processed by modern high-tech nanotechnology, such as grinding, screening, separating, drying and mixing according to the reasonable scientific proportion. It can be widely used in domestic animals, such as chicken, duck, pig, dog, cattle and sheep, fish, shrimp and other aquaculture, but also can be used in the planting and cultivation of vegetables, melon and fruit. In this experiment, the toxicity of sulfur anion additive to the teratogenic susceptibility of SD rats and foetus was studied, which provided some data basis for the further development and utilization of this product. Materials and methods: healthy female SD rats were used to mate with male rats during estrus or estrus, and 10 mg 路kg ~ (-1) 路kg ~ (-3) mg 路kg ~ (-1) were given to female rats at 5-15 days after conception (the most sensitive period during fetal development). The mixture of sulfur and anion additive in 100mg/kg and 1%CMC solution in control group were used to kill the animals on the 20th day after conception. The maternal body weight and feeding quantity were measured, and the clinical symptoms and visceral lesions of pregnant rats were observed. Placenta weight, luteal body number, implantation rate, live fetus rate, fetal weight, body length, viscera, bone development, ratio of female to male, etc. Results: SD female rats were fed with 10 mg / kg sulfur anion additive (10mg / kg) and 33.3 mg / kg (100mg/kg). The feeding amount, body weight, luteal body number, implantation rate and live birth rate of pregnant SD female rats were measured. There was no significant difference in body weight, body length, tail length, anal length, fetal sex ratio, fetal viscera and bone examination between the two groups (P0.05). There was no significant difference between the experimental cardinal number of teratogenicity and the fetal teratogenicity in SD rats, the maternal toxicity was not obvious and the fetus had no obvious teratogenicity. Conclusion: the sulfur anion additive has no toxicity and teratogenicity at the dose of this experiment, but has no obvious toxicity and teratogenicity on the health of the mother and the normal development of the fetal mouse.
【學位授予單位】：延邊大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S859.82

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