【摘要】：多酶飼料是生物飼料的一種,飼料中富含多種飼用酶,能夠在一定程度上消除飼料原料中的抗營養(yǎng)成分或?qū)游飪?nèi)源性酶類起到補充作用,從而有利于改善現(xiàn)有飼料的品質(zhì)、提高飼料的利用率。本研究以酒糟為飼料原料、枯草芽孢桿菌和乳酸菌為發(fā)酵菌種、以植酸酶和甘露聚糖酶等為研究對象,通過微生物發(fā)酵的方式制備新型多酶生物飼料。通過對三株枯草芽孢桿菌L1、L2和L3進行單菌單因素和正交固態(tài)發(fā)酵條件優(yōu)化實驗,分別確定了最佳產(chǎn)酶條件：菌株L1單菌固態(tài)發(fā)酵生產(chǎn)甘露聚糖酶的最優(yōu)培養(yǎng)條件為玉米粉和豆粕粉的比例為8：2、酒糟含量為30%、接種量為每瓶0.8mL(250mL裝10g干料)、溫度35℃、pH自然、料水比為1：1.2、培養(yǎng)時間6d；菌株L2單菌固態(tài)發(fā)酵生產(chǎn)木聚糖酶的最優(yōu)培養(yǎng)條件為玉米粉和豆粕粉的比例為6：4、酒糟含量為40%、接種量為每瓶1mL(250mL裝10g干料)、溫度35℃、pH自然、料水比為1：1、培養(yǎng)時間5d；菌株L3單菌固態(tài)發(fā)酵生產(chǎn)植酸酶的最優(yōu)培養(yǎng)條件為：玉米粉和豆粕粉的比例為5：5、酒糟含量為30%、接種量為每瓶0.8mL(250mL裝10g干料)、溫度37℃、pH7、料水比為1：1.2、培養(yǎng)時間5d。根據(jù)單菌發(fā)酵實驗結(jié)果進行混菌發(fā)酵產(chǎn)酶條件的優(yōu)化實驗,確定混菌發(fā)酵最佳產(chǎn)酶條件：玉米粉與豆粕粉比例為7：3、酒糟含量為37%、麩皮含量為30%、接種量為5%、接種方式為三株枯草芽孢桿菌比例為2：2：1,并將植酸酶產(chǎn)生菌提前接種12 h、發(fā)酵溫度37℃、料水比1：1、pH 8.0、添加硫酸銨3%、尿素1.5%、氯化鈣0.3%、培養(yǎng)時間5d 。將經(jīng)過枯草芽孢桿菌發(fā)酵后的飼料進行乳酸菌發(fā)酵培養(yǎng),以改善飼料氣味、和色澤。優(yōu)化后的乳酸菌菌株R1固態(tài)發(fā)酵條件為裝料量100g、溫度35℃、接種量15%、時間6d。培養(yǎng)結(jié)束后,飼料顏色較培養(yǎng)前由黑色變?yōu)榧t棕色,氣味舒適,消除了枯草芽孢桿菌發(fā)酵氣味和氨味。并分別采用DNS法、地衣酚-鹽酸雙波長差值法和TCA法,測定了發(fā)酵前后飼料中甘露聚糖、木聚糖和植酸磷含量的變化情況。發(fā)酵前后甘露聚糖含量平均下降66.28%、木聚糖含量平均下降12.21%、植酸磷含量平均下降34.09%；又對發(fā)酵后的飼料進行粗蛋白、粗纖維、粗灰分、鈣含量、總磷含量、水分等十個指標的測定,測定結(jié)果符合NY/T 1245-2006,即奶牛用精飼料標準。
[Abstract]:Multi-enzyme feed is a kind of biological feed, which is rich in many kinds of feed enzymes. It can eliminate the anti-nutritional components in feed raw materials to some extent or supplement the endogenous enzymes of animals, thus it can improve the quality of the existing feed. Improve the utilization rate of feed. In this study, using distiller's grains as feed material, Bacillus subtilis and Lactobacillus as fermentation strains, phytase and mannanase as research objects, a new multi-enzyme biofeed was prepared by microbial fermentation. Three strains of Bacillus subtilis L _ (1) L _ (2) and L _ (3) were optimized by single factor and orthogonal solid-state fermentation. The optimum conditions for producing mannanase by solid state fermentation of strain L1 were as follows: the ratio of corn flour to soybean meal was 8: 2, the content of distiller's grains was 30 and the inoculation amount was 10 g dry material per bottle of 0.8mL (250mL). The temperature is 35 鈩，

本文編號：2338044