【摘要】：在豬卵子的體外生產(chǎn)方面,關(guān)于卵子的體外成熟和體外受精技術(shù)目前還存在著不足,特別是豬的受精卵體外生產(chǎn)方面存在復(fù)雜的細(xì)胞質(zhì)成熟、多精子侵入率較高以及前核形成受到抑制等問題。為改善豬卵子體外成熟體系,將轉(zhuǎn)化生長因子β(transforming growth factor-β,簡稱TGFβ)添加到體外成熟的培養(yǎng)液中,研究TGFβ對裸卵和卵丘卵母細(xì)胞復(fù)合體成熟率的影響。在沒有添加TGFβ的體外成熟培養(yǎng)液中,裸卵和卵丘卵母細(xì)胞復(fù)合體被成熟培養(yǎng)24 h,結(jié)果沒有卵子達到M-Ⅱ時期,但是在添加TGFβ的體外成熟培養(yǎng)液中,觀察到有卵子達到M-Ⅱ時期。另一方面,當(dāng)卵丘卵母細(xì)胞復(fù)合體在體外成熟培養(yǎng)時,將TGFβ添加到不同培養(yǎng)階段(0~24 h或24~48 h)的培養(yǎng)液時,卵子的成熟率沒有差別。對裸卵在體外成熟培養(yǎng)24 h時,當(dāng)在前半期添加TGFβ時(0~24 h),卵子的成熟率為59%;當(dāng)在后半期添加TGFβ時(24~48 h),卵子的成熟率為57%。同樣在裸卵組中,當(dāng)在全期添加TGFβ時(0~48 h),卵子的成熟率為27%;當(dāng)無添加TGFβ時,卵子的成熟率為38%。前2組與后2組相比,卵子成熟率有顯著差異。
[Abstract]:In the field of in vitro production of porcine eggs, there are still deficiencies in vitro maturation and fertilization techniques, especially in the complex cytoplasmic maturation of pig fertilized eggs. The rate of polysperm invasion is high and the formation of anterior nucleus is inhibited. In order to improve the in vitro maturation system of porcine eggs, transforming growth factor 尾 (transforming growth factor- 尾 (TGF 尾) was added to the culture medium to study the effect of TGF 尾 on maturation rate of naked eggs and cumulus oocyte complexes. In vitro maturation medium without TGF 尾, nake-oocyte and cumulus oocyte complexes were cultured for 24 h, and no eggs reached M- 鈪，

本文編號：2332858