發(fā)布時(shí)間：2018-11-14 20:07

【摘要】：SPF級(jí)豚鼠因微生物控制嚴(yán)格,飼養(yǎng)條件限制,標(biāo)準(zhǔn)化、規(guī)模化生產(chǎn)繁育需采用操作方便又易滅菌的全價(jià)脫青飼料。本研究對(duì)SPF級(jí)豚鼠脫青飼料中VC的添加和輻照滅菌進(jìn)行了探索,主要進(jìn)行了三方面的試驗(yàn)。結(jié)果如下:1.以普通級(jí)豚鼠為試驗(yàn)對(duì)象,選用乙基纖維素包被VC和單體VC取代青飼料,作為豚鼠脫青飼料維生素C的主要來(lái)源,與青飼料作對(duì)照,進(jìn)行可行性試驗(yàn)。結(jié)果表明:添加乙基纖維素包被VC和單體VC的脫青飼料可基本滿足豚鼠生長(zhǎng)繁殖需要,而且乙基纖維素包被VC效果較好。所以乙基纖維素包被VC作為豚鼠脫青飼料中維生素C的主要來(lái)源可行。2.以SPF級(jí)豚鼠為試驗(yàn)對(duì)象,分別采用乙基纖維素包被VC的添加量分別為500 mg/kg、1000 mg/kg、1500 mg/kg的飼料進(jìn)行飼喂試驗(yàn),從而對(duì)乙基纖維素包被VC的添加量進(jìn)行優(yōu)選。結(jié)果表明:1000 mg/kg,1500 mg/kg較500 mg/kg對(duì)SPF級(jí)豚鼠的生長(zhǎng)繁殖以及多胎繁殖更有利。1500 mg/kg的添加量較1000 mg/kg在生長(zhǎng)繁殖指標(biāo)中未表現(xiàn)出顯著差異,所以結(jié)合飼料成本,選用1000 mg/kg為最優(yōu)添加量。3.對(duì)兩種飼料分別飼喂的SPF級(jí)豚鼠和普通級(jí)豚鼠進(jìn)行了主要臟器重量與臟器系數(shù)的對(duì)比試驗(yàn),進(jìn)一步確定了SPF級(jí)豚鼠脫青飼料的適宜性。4.脫青飼料必須經(jīng)輻照滅菌才可用于SPF級(jí)豚鼠,所以本試驗(yàn)對(duì)15 kGy、30kGy、45 kGy輻照滅菌后飼料對(duì)豚鼠生長(zhǎng)繁殖的影響進(jìn)行了對(duì)比。結(jié)果表明:經(jīng)15 kGy輻照劑量滅菌的飼料在后期出現(xiàn)細(xì)菌生長(zhǎng),保存期不夠長(zhǎng)。經(jīng)30 kGy輻照滅菌的飼料保存期長(zhǎng),飼喂SPF級(jí)豚鼠,生長(zhǎng)繁殖狀況均較穩(wěn)定。經(jīng)45 kGy輻照滅菌的飼料,因有異臭味,豚鼠采食狀況不佳,生長(zhǎng)繁殖指標(biāo)表現(xiàn)出差異性和不穩(wěn)定性。所以初步確定30 kGy為SPF級(jí)豚鼠脫青飼料的最佳輻照劑量,保存期為8個(gè)月。5.通過(guò)對(duì)VC添加量與輻照劑量對(duì)豚鼠生長(zhǎng)繁殖指標(biāo)的分析發(fā)現(xiàn),VC添加量與輻照劑量對(duì)豚鼠生長(zhǎng)繁殖的影響存在交互作用。豚鼠生長(zhǎng)繁殖指標(biāo)的變化是輻照劑量通過(guò)作用于脫青飼料對(duì)乙基纖維素包被VC產(chǎn)生變化,進(jìn)而對(duì)SPF級(jí)豚鼠生長(zhǎng)繁殖產(chǎn)生了影響。
[Abstract]:Due to strict control of microorganism, restriction of feeding conditions, standardization and large-scale production and breeding of SPF grade guinea pigs, it is necessary to adopt full-price degreening feed which is easy to operate and sterilize. In this study, the addition of VC and irradiation sterilization in SPF grade guinea pig forage were studied, and three experiments were carried out. The results are as follows: 1. Taking ordinary guinea pig as experimental object, ethyl cellulose coated with VC and monomer VC was used as the main source of vitamin C in guinea pig degreening feed, and the feasibility test was carried out by using ethyl cellulose coated with VC and monomer VC as the main source of vitamin C in guinea pig degreening feed. The results showed that the degreening feed with ethyl cellulose coated with VC and monomer VC could basically meet the need of growth and reproduction of guinea pigs, and the effect of VC coated with ethyl cellulose was better. So it is feasible to use ethyl cellulose coated with VC as the main source of vitamin C in guinea pig forage. 2. The experiment was carried out on guinea pigs of SPF grade with the addition of ethyl cellulose coated with VC of 500 mg/kg,1000 mg/kg,1500 mg/kg, respectively, in order to optimize the amount of ethyl cellulose coated with VC. The results showed that 1000 mg/kg,1500 mg/kg was more beneficial to the growth and reproduction of SPF grade guinea pigs than 500 mg/kg, and the addition of 1500 mg/kg had no significant difference compared with 1000 mg/kg in the growth and reproduction index. So combined with feed cost, select 1000 mg/kg as the optimal addition amount. 3. The weight and organ coefficient of main organs of SPF guinea pigs and normal guinea pigs fed with two kinds of feed were compared, and the suitability of SPF grade guinea pigs was further determined. 4. In order to be used in SPF grade guinea pigs, degreening forage must be sterilized by irradiation, so the effect of feed on growth and reproduction of guinea pigs after 15 kGy,30kGy,45 kGy irradiation sterilization was compared. The results showed that the bacteria growth appeared in the feed sterilized by 15 kGy irradiation dose, and the preservation period was not long enough. After 30 kGy irradiation sterilization, the feed had long storage life and was fed to SPF grade guinea pigs, and the growth and reproduction status was stable. After 45 kGy of sterilized feed, the feeding condition of guinea pigs was poor because of the odour, and the growth and reproduction index showed difference and instability. Therefore, 30 kGy was determined as the best irradiation dose of SPF grade guinea pig forage, and the storage time was 8 months. By analyzing the effects of VC addition and irradiation dose on growth and reproduction of guinea pigs, it was found that the effects of VC addition and irradiation dose on the growth and reproduction of guinea pigs were interacted. The change of growth and reproduction index of guinea pigs is that irradiation dose can change the VC coated with ethyl cellulose through the action of degreening feed, and then affect the growth and reproduction of SPF grade guinea pigs.
【學(xué)位授予單位】：蘭州理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S816

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