【摘要】：本試驗(yàn)旨在研究包膜酸化劑和小肽螯合鐵及其交互作用對(duì)夏季產(chǎn)蛋高峰期蛋雞生產(chǎn)性能、蛋黃中微量元素含量以及血清免疫和抗氧化指標(biāo)的影響。試驗(yàn)采用2×4雙因子完全隨機(jī)試驗(yàn)設(shè)計(jì),其中飼糧中包膜酸化劑設(shè)2個(gè)添加水平,小肽螯合鐵設(shè)4個(gè)添加水平。選用576只38周齡健康羅曼粉殼蛋雞,隨機(jī)分成8個(gè)組,每組6個(gè)重復(fù),每個(gè)重復(fù)12只雞。A、B、C和D組飼糧分別在基礎(chǔ)飼糧中添加0 mg/kg包膜酸化劑以及0(對(duì)照組)、0.04%、0.08%和0.12%小肽螯合鐵(鐵含量分別為0、60、120和180 mg/kg);E、F、G和H組飼糧分別在基礎(chǔ)飼糧中添加300 mg/kg包膜酸化劑以及0、0.04%、0.08%和0.12%的小肽螯合鐵(鐵含量分別為0、60、120和180 mg/kg)。預(yù)試期1周,正試期6周。結(jié)果表明:1)包膜酸化劑和小肽螯合鐵及其交互作用對(duì)第3周和第6周蛋雞的平均日采食量、產(chǎn)蛋率和料蛋比均無顯著影響(P0.05)。G組第3周和第6周的平均日采食量均顯著高于對(duì)照組和C、D組(P0.05);B組第6周的產(chǎn)蛋率顯著高于對(duì)照組和D、E組(P0.05),且料蛋比顯著低于D組(P0.05)。包膜酸化劑對(duì)第3周末蛋雞的蛋重、第6周末的蛋黃指數(shù)和哈氏單位有顯著影響(P0.05);小肽螯合鐵對(duì)第6周末的蛋黃指數(shù)有顯著影響(P0.05),小肽螯合鐵和包膜酸化劑的交互作用對(duì)第6周末的蛋黃指數(shù)有極顯著影響(P0.01),對(duì)第6周末的哈氏單位有顯著影響(P0.05)。第3周末,G組的蛋重顯著高于B組(P0.05),G和H組的蛋黃指數(shù)顯著高于C組(P0.05);第6周末,C、D和G組的蛋重顯著高于對(duì)照組(P0.05),F組的蛋黃指數(shù)顯著高于C、D和E組(P0.05),E、F和G組的哈氏單位顯著高于D組(P0.05)。2)小肽螯合鐵及包膜酸化劑和小肽螯合鐵的交互作用對(duì)第3周和第6周末蛋雞蛋黃中鐵元素含量有極顯著影響(P0.01)。B、C、D、F、G和H組第3周和第6周末蛋黃中鐵元素含量極顯著高于對(duì)照組和E組(P0.01),且H組第3周末蛋黃中鐵元素含量顯著高于B和F組(P0.05);H組第3周末蛋黃中鋅元素含量顯著高于F組(P0.05),B組第6周末蛋黃中鋅元素含量顯著高于D組(P0.05)。3)包膜酸化劑對(duì)蛋雞血清中總超氧化物歧化酶(T-SOD)活性有極顯著影響(P0.01),對(duì)血清丙二醛(MDA)含量有顯著影響(P0.05);包膜酸化劑和小肽螯合鐵的交互作用對(duì)血清T-SOD活性有極顯著影響(P0.01)。C組血清免疫球蛋白M(Ig M)含量顯著高于對(duì)照組(P0.05),D、E、F、G和H組血清T-SOD活性極顯著高于對(duì)照組(P0.01);B、F和G組血清MDA含量顯著低于對(duì)照組和E組(P0.05)。綜合考慮,飼糧中單獨(dú)添加0.04%和0.08%的小肽螯合鐵或與300 mg/kg包膜酸化劑復(fù)合添加均有利于維持和延長(zhǎng)蛋雞夏季的產(chǎn)蛋高峰期,改善蛋品質(zhì),增加蛋中鐵元素的富集,增強(qiáng)機(jī)體抗氧化能力。
[Abstract]:The purpose of this experiment was to study the effects of encapsulated acidifier and small peptide chelate iron and their interaction on the performance of laying hens in the peak period of laying in summer, the content of trace elements in yolk, and the indexes of serum immunity and antioxidation. The experiment was designed by 2 脳 4 double factor complete random experiment, in which 2 levels were added to the dietary capsule acidifier and 4 levels were added to the small peptide chelate iron. A total of 576 healthy 38-week-old Roman powder shell laying hens were randomly divided into 8 groups with 6 replicates in each group and 12 chickens in each replica. Groups A, B, C and D were fed with 0 mg/kg capsule acidifier and 0 (control group), respectively. 0.04% and 0.12% small peptide chelate iron (iron content were 0 ~ 60120 and 180 mg/kg, respectively); The diets of group E FG and group H were supplemented with 300 mg/kg encapsulated acidifier and 0. 04% and 0. 12% of small peptide chelate iron (iron content was 0 ~ 60120 and 180 mg/kg, respectively). The pretest period was 1 week and the positive test period was 6 weeks. The results showed that: 1) the average daily feed intake of laying hens at the 3rd and 6th week was determined by coating acidifier, iron chelate with small peptide and their interaction. There was no significant difference in egg laying rate and feed to egg ratio (P0.05). The average daily feed intake of). G group was significantly higher than that of control group and Cedar D group (P0.05). The egg laying rate of group B was significantly higher than that of control group and DNE group (P0.05), and the ratio of feed to egg was significantly lower than that of group D (P0.05). The coating acidifier had significant effects on egg weight at the end of the 3rd week and the egg yolk index and the hardy unit at the end of the 6th week (P0.05). Iron chelate had a significant effect on yolk index at the end of the 6th week (P0.05), and the interaction between ferric chelate and encapsulated acidifier had a significant effect on the yolk index at the end of the 6th week (P0.01). It had a significant effect on the Hardy units at the end of the 6th week (P0.05). At the end of the third week, the egg weight of group G was significantly higher than that of group B (P0.05) (P0.05) and the yolk index of group H was significantly higher than that of group C (P0.05). At the end of the 6th week, the egg weight of group C D and G was significantly higher than that of control group (P0.05), E,). The yolk index of group), F was significantly higher than that of group C D and group E (P0.05), E,). The interaction of small peptide chelate iron, encapsulated acidifier and small peptide chelate iron in F and G groups was significantly higher than that in D group (P0.05). The iron content in egg yolk at the 3rd and 6th week was significantly affected by the interaction of the small peptide chelate iron and the small peptide chelate iron in the egg yolk at the 3rd and 6th week. (P0.01). B, The content of iron in yolk of group C and H was significantly higher than that of control group and group E at the 3rd and 6th week (P0.01), and the content of iron in yolk of group H was significantly higher than that of group B and F at the end of the third week (P0.05). The content of zinc in yolk of group H was significantly higher than that of group F at the end of the third week (P0.05). The content of zinc in yolk of group B was significantly higher than that in group D at the end of the 6th week (P0.05). The serum activity of total superoxide dismutase (T-SOD) was significantly affected by the coating acidifier (P0.01). The content of malondialdehyde (MDA) in serum was significantly affected (P0.05). The interaction between encapsulated acidifier and ferric chelate had a significant effect on the activity of serum T-SOD (P0.01). C group was significantly higher than the control group (P0.05), the content of M (Ig M) in serum of P0.01). C group was significantly higher than that of control group (P0.05). Serum T-SOD activity in G and H groups was significantly higher than that in control group (P0.01). The levels of serum MDA in group B F and group G were significantly lower than those in group B and E (P 0.05). Considering comprehensively, adding 0.04% and 0.08% ferric chelate of small peptide or combined with 300 mg/kg coating acidifier in diet is beneficial to maintain and prolong the peak period of laying in summer and improve egg quality. Increase the enrichment of iron in eggs and enhance the antioxidant capacity of the body.
【作者單位】： 湖南農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院湖南畜禽安全生產(chǎn)協(xié)同創(chuàng)新中心;奧格生物技術(shù)(上海)有限公司;
【基金】：湖南農(nóng)業(yè)大學(xué)產(chǎn)學(xué)研合作項(xiàng)目(11110,13098)
【分類號(hào)】：S831.5

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