【摘要】：為了研究微生物發(fā)酵床不同發(fā)酵等級(jí)墊料中大腸桿菌的分布及其特性,試驗(yàn)采集了微生物發(fā)酵床豬舍不同發(fā)酵程度的墊料,進(jìn)行大腸桿菌的分離鑒定和藥敏試驗(yàn),分析了發(fā)酵床中大腸桿菌的致病性和抗生素抗性。試驗(yàn)結(jié)果表明,在10-5稀釋倍數(shù)下,僅在墊料發(fā)酵級(jí)別為一級(jí)的墊料中,即墊料使用時(shí)間較短、發(fā)酵程度較低(0△E≤7.63)的條件下,分離到41株大腸桿菌;而在發(fā)酵程度較高的墊料中,即墊料發(fā)酵程度二級(jí)以上(△E7.63)的環(huán)境中未分離到大腸桿菌。隨著發(fā)酵的進(jìn)行,發(fā)酵床中大腸桿菌數(shù)量逐漸減少。41株大腸桿菌中含有致瀉性大腸桿菌15株,其中具有熱穩(wěn)定腸毒素(astA)基因的大腸桿菌9株,占總數(shù)的22%,含耐藥性因子(sepA)基因的大腸桿菌2株,占總數(shù)的4.8%;耐強(qiáng)力霉素的大腸桿菌11株,占總數(shù)的27%。含有sepA基因的大腸桿菌具有強(qiáng)耐藥性。從以上結(jié)果來(lái)看,微生物發(fā)酵床對(duì)豬舍大腸桿菌具有抑制作用,可為發(fā)酵床的推廣應(yīng)用及墊料再利用提供一定依據(jù)。
[Abstract]:In order to study the distribution and characteristics of Escherichia coli in different fermentation grades of microbial fermenting bed, the paper collected the bedding material of different fermentation degree in microbial fermentation bed, and carried out the isolation and identification of Escherichia coli and drug sensitivity test. The pathogenicity and antibiotic resistance of Escherichia coli in fermentation bed were analyzed. The results showed that 41 strains of Escherichia coli were isolated under the condition of 10-5 dilution times, only when the fermentation grade of the gasket was the first grade, that is, the use time of the cushion was shorter and the fermentation degree was lower (0 E 鈮，

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