【摘要】：為了制備并評(píng)價(jià)大熊貓(Ailuropoda melanoleuca)重組干擾素γ(IFN-γ)聚氰基丙烯酸正丁酯(PBCA)納米球,本研究利用蛋白質(zhì)原核表達(dá)技術(shù),獲得了重組大熊貓IFN-γ蛋白,然后以PBCA為載藥材料,采用乳化聚合法制備了大熊貓干擾素γ納米微球(IFNγ-PBCA-NS),最后借助感染大熊貓流感病毒A/Panda/Sichuan/01/2011(H1N1)的昆明小鼠(Mus musculus)模型,通過灌胃和皮下注射藥物初步評(píng)價(jià)了IFNγ-PBCA-NS的藥效。結(jié)果表明,大熊貓IFN-γ的蛋白分子量約為33.5 ku,所制備的大熊貓IFNγ-PBCA-NS外觀規(guī)整,粒徑在50~200 nm之間,跨距0.55,大小較均勻,包封率為56.7%,載藥量為0.86%。灌胃和注射兩種給藥途徑中,IFNγ-PBCA-NS組小鼠的生命延長率均顯著高于IFN-γ組(P0.05或P0.01)。顯示IFNγ-PBCA-NS在小鼠體內(nèi)有更好的緩釋和抗病毒作用,可為進(jìn)一步研究制備大熊貓多肽類藥物微粒提供參考。
[Abstract]:In order to prepare and evaluate giant panda (Ailuropoda melanoleuca) recombinant interferon 緯 (IFN- 緯) polybutyl cyanoacrylate (PBCA) nanospheres, the recombinant giant panda IFN- 緯 protein was obtained by using prokaryotic protein expression technique, and then PBCA was used as drug carrier. Giant panda interferon 緯 nanospheres (IFN 緯-PBCA-NS) were prepared by emulsion polymerization. Finally, the drug efficacy of IFN 緯-PBCA-NS was evaluated by intragastric administration and subcutaneous injection of IFN 緯-PBCA-NS by (Mus musculus) model of Kunming mice infected with giant panda influenza virus A/Panda/Sichuan/01/2011 (H1N1). The results showed that the giant panda IFN 緯-PBCA-NS prepared by the giant panda IFN- 緯 with molecular weight of about 33. 5 ku, had a regular appearance, the diameter was between 50 ~ 200 nm, the span was 0. 55, the size was uniform, the entrapment efficiency was 56. 7, and the drug loading was 0. 86%. The prolongation rate of life in IFN 緯 PBCA-NS group was significantly higher than that in IFN- 緯 group (P0.05 or P0.01). The results showed that IFN 緯-PBCA-NS had better sustained release and antiviral effects in mice, which could provide a reference for further study on the preparation of giant panda polypeptide drug particles.
【作者單位】： 四川農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;四川農(nóng)業(yè)大學(xué)動(dòng)物生物技術(shù)中心;中國保護(hù)大熊貓研究中心;
【基金】：四川省科技計(jì)劃項(xiàng)目(No.2012JQ0037)
【分類號(hào)】：S859.5

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