【摘要】：本試驗旨在探究綠茶粉及綠茶多酚對犬的抗氧化作用。選用5月齡雜交幼犬15只,隨機分為3個組,每組5只。對照組飼喂基礎(chǔ)飼糧,綠茶粉組飼喂基礎(chǔ)飼糧+1.0%的綠茶粉,綠茶多酚組飼喂基礎(chǔ)飼糧+0.25%的綠茶多酚。試驗期為84 d。結(jié)果表明:與試驗開始時相比,試驗結(jié)束時對照組犬體重增長了162%,綠茶粉組犬體重增長了101%,綠茶多酚組犬體重增長了132%,且綠茶粉組和綠茶多酚組試驗結(jié)束時犬體重顯著低于對照組(P0.05),比對照組分別降低了61%和30%。試驗第84天,綠茶粉組及綠茶多酚組犬血清中谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)、乳酸脫氫酶(LDH)活性均顯著或極顯著低于對照組(P0.05或P0.01),血清超氧化物歧化酶(SOD)、谷胱甘肽過氧化物酶(GSH-Px)活性極顯著高于對照組(P0.01),血清丙二醛(MDA)含量極顯著低于對照組(P0.01),肝臟抗氧化基因[谷氨酸半胱氨酸連接酶催化亞基(GCLC)、谷氨酸半胱氨酸連接酶修飾亞基(GCLM)、血紅素氧合酶1(HO-1)、過氧化氫酶(CAT)]和Ⅱ相解毒酶基因[谷胱甘肽硫轉(zhuǎn)移酶M1(GSTM1)和醌氧化還原酶1(NQO1)]的mRNA相對表達量顯著高于對照組(P0.05)。綜上所述,在本試驗條件下,在犬飼糧中添加1.0%的綠茶粉或0.25%的綠茶多酚均具有保護肝臟、抗氧化、促進Ⅱ相解毒酶基因表達及控制體重的作用。
[Abstract]:The aim of this study was to investigate the anti-oxidation effect of green tea powder and green tea polyphenols on dogs. Fifteen 5 month old crossbred puppies were randomly divided into 3 groups with 5 puppies in each group. The control group was fed with basic diet, the green tea powder group was fed with 1.0% green tea powder and the green tea polyphenol group was fed with 0.25% green tea polyphenol. The experimental period is 84 days. The results show that: compared with the beginning of the experiment, At the end of the experiment, the weight of control group increased 162%, green tea powder group increased 101%, green tea polyphenol group increased 132%, and the weight of green tea powder group and green tea polyphenol group was significantly lower than that of control group at the end of the experiment (P0.05), which decreased by 61% and 30% respectively. On the 84th day of the experiment, The activity of serum alanine aminotransferase (ALT), glutamic oxalacetic transaminase (AST), lactate dehydrogenase (LDH) in green tea powder group and green tea polyphenol group was significantly or extremely lower than that in control group (P0.05 or P0.01), and the activity of serum superoxide dismutase (SOD),) glutathione peroxidase (GSH-Px) was significantly lower than that in control group (P0.01). The content of malondialdehyde (MDA) in serum was significantly lower than that in the control group (P0.01). The liver antioxidant gene [glutamate cysteine ligase catalytic subunit (GCLC), glutamate cysteine ligase modified (GCLM), heme oxygenation] was significantly higher than that of the control group (P0.01). The relative expression of mRNA of enzyme 1 (HO-1), catalase (CAT)] and phase 鈪，

本文編號：2290724