【摘要】：鉛是應(yīng)用最廣泛的重金屬之一,長期處于鉛暴露環(huán)境是引起鉛中毒的主要原因,臨床上鉛中毒多見于職業(yè)病。進(jìn)入機(jī)體后,鉛會沉積在全身多個部位,對多個組織器官造成損害。牛磺酸是一種具有多項(xiàng)調(diào)節(jié)功能的內(nèi)源性抗損傷物質(zhì),在動物體內(nèi)廣泛存在,本研究以Wistar大鼠為研究對象,將大鼠分為空白對照組、染鉛組、�；撬峤M和牛磺酸預(yù)防組等若干組,進(jìn)行不同處理后,從以下四個部分開展研究工作:1.�；撬釋θ俱U大鼠生長發(fā)育的影響將大鼠分組后進(jìn)行不同處理,每周記錄各組大鼠的體重?cái)?shù)據(jù),于實(shí)驗(yàn)?zāi)┻M(jìn)行大鼠腹主動脈采血制備血漿,血樣消解后檢測血鉛含量;將心、肝、脾、肺、腎和睪丸等器官分離摘取出來,測定各組大鼠臟器系數(shù)。結(jié)果發(fā)現(xiàn)各組大鼠體重變化無顯著差異(P0.05),鉛對大鼠脾、肺、腎和睪丸的臟器系數(shù)有顯著影響(P0.05),�；撬犷A(yù)防對染鉛大鼠腎和肺的發(fā)育具有明顯的改善作用(P0.05);染鉛組大鼠血鉛含量明顯高于空白對照組(P0.05),且與染鉛組相比,牛磺酸預(yù)防組大鼠血鉛濃度明顯下降(P0.05)。2.牛磺酸對染鉛大鼠肝、腎、睪丸結(jié)構(gòu)損傷的改善作用鉛進(jìn)入動物機(jī)體后很難被機(jī)體排出而在各組織器官處蓄積下來,對機(jī)體的組織、器官造成損害。本部分研究主要通過制備石蠟切片和透射電鏡超薄切片,從病理組織學(xué)變化來探討牛磺酸對鉛中毒的預(yù)防作用。光學(xué)顯微鏡和透射電鏡觀察發(fā)現(xiàn)染鉛組大鼠肝、腎、睪丸組織都發(fā)生了不同程度的損傷;與染鉛組相比,牛磺酸預(yù)防組大鼠組織損傷有所改善,證實(shí)了�；撬釋︺U中毒引起的大鼠組織損傷具有預(yù)防作用。3.�；撬釋θ俱U大鼠氧化損傷的預(yù)防作用鉛主要通過氧化應(yīng)激機(jī)制,引起機(jī)體過氧化過程,生成活性氧自由基(ROS),對機(jī)體的組織器官造成損害。本部分研究主要從測定組織和血液抗氧化生化指標(biāo)來探討�；撬釋︺U中毒的預(yù)防作用。將分離出來的大鼠組織和采集的血液分別制備處理為組織勻漿和血漿,樣品前處理完成后進(jìn)行組織勻漿和血漿中GSH、GSH-Px、MDA、SOD的檢測。結(jié)果發(fā)現(xiàn)染鉛組大鼠各組織和血漿中GSH、GSH-Px、SOD濃度均最低,補(bǔ)充�；撬岷鬂舛扔兴�,但仍低于空白對照組和牛磺酸組Ⅰ、牛磺酸組Ⅱ;染鉛組大鼠MDA含量均最高,補(bǔ)充�；撬岷鬂舛扔兴陆�,但仍高于空白對照組和牛磺酸組Ⅰ、�；撬峤MⅡ,說明�；撬犷A(yù)防組大鼠的各項(xiàng)指標(biāo)在一定程度上得到了改善。4.牛磺酸對染鉛大鼠肝組織細(xì)胞凋亡的影響本部分研究應(yīng)用TUNEL法檢測各組大鼠肝細(xì)胞凋亡情況,探討�；撬犷A(yù)防對染鉛大鼠肝細(xì)胞凋亡的改善作用。結(jié)果顯示,鉛中毒會導(dǎo)致大鼠肝細(xì)胞凋亡,而經(jīng)過�；撬犷A(yù)防后,細(xì)胞凋亡情況有所好轉(zhuǎn)。以上結(jié)果表明,�；撬釋Υ笫筱U中毒有一定的預(yù)防作用。
[Abstract]:Lead is one of the most widely used heavy metals. Exposure to lead for a long time is the main cause of lead poisoning. After entering the body, lead deposits in multiple parts of the body, causing damage to multiple tissues and organs. Taurine (taurine) is an endogenous anti-injury substance with multiple regulatory functions, which is widely present in animals. In this study, Wistar rats were divided into blank control group and lead exposed group. Taurine group and taurine prevention group and other groups, after different treatment, from the following four parts of the research work: 1. Effects of taurine on the growth and Development of lead exposed Rats the rats were divided into groups, and the body weight data of each group were recorded weekly. At the end of the experiment, blood samples were collected from the abdominal aorta of rats to prepare plasma, and blood samples were digested to detect the content of blood lead. Liver, spleen, lung, kidney and testis were isolated and the visceral coefficients were measured. The results showed that there was no significant difference in body weight change in each group (P0.05). The visceral coefficients of kidney and testis were significantly affected (P0.05), and taurine could significantly improve the development of kidney and lung of lead-exposed rats (P0.05), and the blood lead content of lead-exposed rats was significantly higher than that of blank control group (P0.05), and compared with lead-exposed rats, taurine had a significant effect on the development of kidney and lung in lead-exposed rats (P0.05). The concentration of blood lead in taurine prevention group decreased significantly (P0.05). Effects of taurine on the damage of liver, kidney and testis in lead exposed rats; lead is difficult to be excreted by the body and accumulates in various tissues and organs, which causes damage to the tissues and organs of the body. In this part, the preventive effect of taurine on lead poisoning was studied by preparing paraffin sections and ultrathin sections of transmission electron microscope. The results of optical microscope and transmission electron microscope showed that the liver, kidney and testicular tissues of the lead exposed rats were damaged to varying degrees, and that of the taurine pretreated group was better than that of the lead exposed group. It is proved that taurine has preventive effect on tissue injury induced by lead poisoning in rats. Prevention of oxidative damage induced by taurine in lead exposed rats the oxidative stress mechanism led to the formation of reactive oxygen free radical (ROS),) which caused damage to tissues and organs of the body through the mechanism of oxidative stress. In this part, the preventive effect of taurine on lead poisoning was studied by measuring the biochemical indexes of tissue and blood antioxidant. The isolated rat tissues and blood samples were prepared and treated into tissue homogenate and plasma respectively. GSH,GSH-Px,MDA,SOD in tissue homogenate and plasma were detected after sample pretreatment. The results showed that the concentration of GSH,GSH-Px,SOD was the lowest in all tissues and plasma of lead-exposed rats, and increased after taurine supplementation, but still lower than that of control group, taurine group 鈪，

本文編號：2280621