【摘要】：運用代謝組學(xué)方法研究神經(jīng)肽Kisspeptin對熱應(yīng)激大鼠的保護作用。本試驗通過建立熱應(yīng)激大鼠模型,基于氣相色譜-飛行時間質(zhì)譜聯(lián)用技術(shù)(GC/TOFMS)代謝組學(xué)方法檢測大鼠血清中內(nèi)源性代謝物,并采用主成分分析及偏最小二乘判別法分析對照組、熱應(yīng)激組和Kisspeptin干預(yù)組之間的代謝物差異,篩選出潛在的生物標(biāo)志物。代謝組學(xué)結(jié)果顯示熱應(yīng)激組大鼠的血清代謝譜與正常對照組大鼠明顯不同,而Kisspeptin干預(yù)后大鼠的血清代謝譜有趨于正常的趨勢。生化代謝通路分析結(jié)果顯示熱應(yīng)激引起大鼠三羧酸循環(huán)、糖代謝、氨基酸和核苷酸代謝異常,而熱應(yīng)激后給與Kisspeptin處理可緩解上述代謝通路的紊亂并趨于正常狀態(tài)。此外,氧化應(yīng)激指標(biāo)檢測結(jié)果顯示給與Kisspeptin處理可降低熱應(yīng)激大鼠血清中丙二醛(MDA)含量,并提高超氧化酶歧化酶(T-SOD)、總抗氧化能力(T-AOC)和谷胱甘肽過氧化物酶(GSH-Px)的活力,該結(jié)果表明Kisspeptin在一定程度上緩解了熱應(yīng)激誘導(dǎo)的氧化應(yīng)激。該研究從代謝及抗氧化的角度研究了Kisspeptin對熱應(yīng)激大鼠的保護作用,為動物健康養(yǎng)殖和開發(fā)抗熱應(yīng)激的安全生物制品提供試驗依據(jù)。
[Abstract]:The protective effect of neuropeptide Kisspeptin on heat stress rats was studied by metabonomics. In this experiment, the heat stress rat model was established, and the endogenous metabolites in serum were detected by gas chromatography-time-of-flight mass spectrometry (GC/TOFMS), and the control group was analyzed by principal component analysis and partial least-squares discriminant analysis. The differences of metabolites between heat stress group and Kisspeptin intervention group were used to screen out potential biomarkers. The results of metabonomics showed that the serum metabolic spectrum of heat stress group was obviously different from that of normal control group, but the serum metabolic spectrum of Kisspeptin group tended to be normal. The results of biochemical metabolic pathway analysis showed that heat stress induced abnormal metabolism of tricarboxylic acid, glucose, amino acid and nucleotide in rats. However, Kisspeptin treatment after heat stress alleviated the disorder of these metabolic pathways and tended to be normal. In addition, the results of oxidative stress index showed that Kisspeptin treatment could decrease the content of malondialdehyde (MDA) in serum and increase the activities of superoxidase dismutase (T-SOD), total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px). The results showed that Kisspeptin alleviated oxidative stress induced by heat stress to some extent. In this study, the protective effects of Kisspeptin on heat stress rats were studied from the point of view of metabolism and antioxidation, which provided experimental basis for healthy breeding of animals and development of safe biological products resistant to heat stress.
【作者單位】： 南京農(nóng)業(yè)大學(xué)動物醫(yī)學(xué)院;
【分類號】：S852.3

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