【摘要】：試驗旨在探究胰島素樣生長因子結(jié)合蛋白2(insulin-like growth factor binding protein 2,IGFBP2)基因遺傳變異對豬肉質(zhì)性狀的影響,為豬品種選育提供遺傳學(xué)依據(jù)。參考Gen Bank中豬IGFBP2基因序列(登錄號:BV727778)設(shè)計1對引物,采用PCR-RFLP技術(shù)檢測IGFBP2基因在蘇姜豬、姜曲海豬、長白豬中的遺傳多態(tài)性,并分析基因座多態(tài)性與豬肉質(zhì)性狀的相關(guān)性。結(jié)果發(fā)現(xiàn),3個豬種IGFBP2基因內(nèi)含子3均具有MspⅠ酶切多態(tài)性,分別檢測到3種基因型:AA、AB和BB;χ2適合性檢驗顯示,3個豬種IGFBP2基因基因型分布均處于HardyWeinberg平衡狀態(tài)(P0.05);多態(tài)信息含量(PIC)分析顯示,3個豬種均為中度多態(tài)。IGFBP2基因不同基因型與肉質(zhì)性狀關(guān)聯(lián)分析顯示,3個豬種不同基因型肉色、肌內(nèi)脂肪含量、大理石紋差異顯著,AA基因型肉色(姜曲海豬除外)、肌內(nèi)脂肪含量均顯著高于AB、BB基因型(P0.05);AA、AB基因型大理石紋顯著高于BB基因型(P0.05),其余指標(biāo)間差異不顯著(P0.05)。綜上,蘇姜豬、姜曲海豬、長白豬IGFBP2基因內(nèi)含子3多態(tài)性對肉質(zhì)性狀影響顯著,可作為潛在影響豬肉質(zhì)性狀的候選基因。
[Abstract]:The aim of the experiment was to investigate the effect of genetic variation of insulin-like growth factor binding protein 2 (insulin-like growth factor binding protein _ 2 IGFBP2) gene on pork quality traits, and to provide genetic basis for pig breeding. A pair of primers were designed with reference to pig IGFBP2 gene sequence (accession number: BV727778) in Gen Bank. The genetic polymorphism of IGFBP2 gene in Sujiang pig, Jiangqu Hai pig and Landrace pig was detected by PCR-RFLP technique, and the correlation between locus polymorphism and pig meat quality traits was analyzed. The results showed that intron 3 of IGFBP2 gene of all three pig breeds had Msp 鈪，

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