【摘要】：目的比較動物源沙門氏菌多位點序列分型(MLST)與血清分型的差別,獲得動物源沙門氏菌在山東省的分布規(guī)律。方法從山東部分地區(qū)分離78株雞源沙門氏菌、56株鴨源沙門氏菌和20株豬源的沙門氏菌,PCR擴增七段沙門氏菌內(nèi)部保守的片段序列進行多位點序列分型,同時用玻片凝集法分析其血清型。結果血清分析結果表明,雞源沙門氏菌檢出6種血清型,其中腸炎沙門氏菌占88.5%、印第安納沙門氏菌占5.1%、湯卜遜沙門氏菌占2.6%、鼠傷寒沙門氏菌占1.3%、山夫登堡沙門氏菌占1.3%、阿格瑪沙門氏菌占1.3%;鴨源沙門氏菌檢出2種血清型,其中腸炎沙門氏菌占67.9%、鼠傷寒沙門氏菌占32.1%;豬源沙門氏菌檢出3種血清型,其中鼠傷寒沙門氏菌占65%、德貝兒沙門氏菌占20%、腸炎沙門氏菌占15%。通過MLST分型,雞源檢出7種ST型:ST11、ST19、ST26、ST128、ST14、ST17和New1;鴨源檢出3種ST型:ST11、ST19和New2;豬源檢出3種:ST34、ST40和ST3007。結論整體來看,分離菌的血清型和ST型數(shù)量比較接近,二者分型能力相近。
[Abstract]:Objective to compare the difference between (MLST) and serological typing of animal origin Salmonella and to obtain the distribution of animal origin Salmonella in Shandong Province. Methods 78 strains of Chicken Salmonella, 56 strains of Duck Salmonella and 20 strains of Porcine Salmonella were isolated from some parts of Shandong Province. The conserved fragments of seven segments of Salmonella were amplified by PCR. At the same time, the serum type was analyzed by slide agglutination method. Results the results of serum analysis showed that 6 serotypes of salmonella from chicken were detected. Among them, Salmonella enteritidis accounted for 88.5m, Indiana Salmonella for 5.1, Thomson Salmonella for 2.6am, Salmonella typhimurium for 1.3, Salmonella from Shanftenberg for 1.3amentum for 1.3and salmonella from duck for two serotypes. Salmonella enteritidis accounted for 67.9%, Salmonella typhimurium accounted for 32.1, Salmonella isolated from swine accounted for three serotypes, Salmonella typhimurium accounted for 65%, Deber Salmonella accounted for 20%, Salmonella enteritis accounted for 15%. By MLST typing, 7 ST types were detected in chicken, 3 ST types in ST11,ST19,ST26,ST128,ST14,ST17 and New1; duck and 3 ST types in ST11,ST19 and New2; pigs: ST34,ST40 and ST3007.. Conclusion on the whole, the serotypes and ST serotypes of isolated bacteria were similar, and the ability of typing was similar.
【作者單位】： 山東省泰安市動物衛(wèi)生監(jiān)督所;山東省農(nóng)業(yè)科學院畜牧獸醫(yī)研究所;山東農(nóng)業(yè)大學動物科學院;山東省日照市動物疫病預防控制中心;山東華宏生物工程有限公司;
【基金】：山東省農(nóng)業(yè)科學院科技創(chuàng)新工程(CXGC2016A10) 山東省農(nóng)業(yè)重大應用技術創(chuàng)新項目(SDAIT-11-09)~~
【分類號】：S852.6

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