濟寧青山羊出生后胃腸道中GnRH及GnRHR的發(fā)育性變化
發(fā)布時間：2018-10-12 11:34
【摘要】：原產自我國的濟寧青山羊是一種獨特的羔皮用山羊品種,其特性是四季發(fā)情,性成熟早,母羊在3月齡、公羊在4月齡發(fā)育至性成熟。濟寧青山羊的初情期開始于3-4月齡,部分發(fā)育較早的個體于2月齡開始初情期,其發(fā)情周期為17.50d±0.50d,發(fā)情持續(xù)時間為1-2d,妊娠期約為147.00d±2.50 d,平均146d。濟寧青山羊的繁殖率高,其羔羊的成活率高達95%。其性情溫馴易于管理,遺傳性穩(wěn)定,適應能力強,耐粗飼,具有很高的經濟價值,現已被列入《中國羊品種志》,屬于優(yōu)良的地方山羊品種。研究濟寧青山羊胃腸道促性腺激素釋放激素(GnRH)及促性腺激素釋放激素受體(GnRHR)分布的增齡性變化對于了解GnRH及GnRHR對動物生后發(fā)育階段消化道的功能產生的影響提供了必要的理論依據。為了解濟寧青山羊生后發(fā)育時期胃腸道內GnRH和GnRHR的分布和發(fā)育性變化。本試驗運用免疫組織化學(SABC)的方法檢測了濟寧青山羊出生當天(0日齡)、30日齡、60日齡、90日齡、120日齡、150日齡、180日齡胃腸道中GnRH和GnRHR免疫陽性細胞的動態(tài)分布。在胃腸道內,GnRH和GnRHR免疫陽性產物均定位在胞質中,為黃色或者棕黃色,胞核陰性,陰性對照無陽性著色。0日齡,GnRH免疫陽性產物在皺胃中分布在胃底腺的基底部、小腸的絨毛上皮之間、大腸的大腸腺杯狀細胞之間;30日齡后,小腸腺上皮細胞中也出現了分布,其他各部位的免疫陽性物質明顯增多,并且保持不斷的增長趨勢,皺胃中GnRH免疫陽性產物在60日齡達到峰值,其他部位在90日齡達到峰值。在濟寧青山羊的0日齡時,GnRHR免疫陽性產物在胃底腺胃小凹上皮細胞與壁細胞中、小腸的絨毛上皮細胞與十二指腸腺上皮細胞中、大腸黏膜上皮細胞中;30日齡后,大腸腺的腺上皮細胞中也出現了分布,隨后保持快速增長的趨勢直至90日齡達到峰值。自0日齡-90日齡,GnRH和GnRHR免疫陽性細胞平均積分光密度增加均極顯著(P0.01),90日齡后維持相對穩(wěn)定(P0.05)。綜上所述,筆者推測濟寧青山羊胃腸道還可以通過自分泌或旁分泌的形式合成與分泌GnRH;GnRH可能通過GnRHR的介導促進了胃腸道的生后發(fā)育及成熟過程。
[Abstract]:Jining Grey Goat (Jining), which originated from China, is a unique goat breed for lamb skin, which is characterized by four seasons of estrus, early sexual maturity, 3 months old ewe and 4 months old male sheep. The initial estrous period of Jining green goat begins at 3-4 months old, and some of the early developing individuals begin their early estrous period at the age of 2 months. The estrus cycle is 17.50 days 鹵0.50 days, the estrus duration is 1-2 days, and the gestation period is about 147.00 鹵2.50 days, with an average of 146 days. The reproduction rate of Jining green goat is high, and the survival rate of lamb is up to 95%. Its temperament is easy to manage, genetic stability, strong adaptability, tolerance to coarse feeding, has a high economic value, has been listed in the "Chinese Sheep Variety", belong to the excellent local goat breeds. Study on the Aging changes of Gastrointestinal Gonadotropin releasing hormone (GnRH) and Gonadotropin releasing hormone receptor (GnRHR) Distribution in Jining Green Goat provides a way to understand the effects of GnRH and GnRHR on the gastrointestinal function during postnatal development of animals The necessary theoretical basis is given. To investigate the distribution and developmental changes of GnRH and GnRHR in gastrointestinal tract of Jining green goat during postnatal development. The dynamic distribution of GnRH and GnRHR immunoreactive cells in the gastrointestinal tract of Jining green goats on the day of birth (0 days old), 30 days old, 60 days old, 90 days old, 120 days old, 150 days old and 180 days old was detected by immunohistochemical (SABC) method. In the gastrointestinal tract, GnRH and GnRHR immunoreactive products were located in the cytoplasm, yellow or brown, negative in nucleus, and no positive staining in negative control. At 0 days of age, GnRH immunoreactive products were distributed in the basal base of fundus gland in the abomasum. Between villous epithelium of the small intestine and goblet cells of the large intestine, after 30 days of age, the epithelial cells of the small intestine also appeared to be distributed, and the immunoreactive substances in the other parts of the intestine increased obviously and kept the increasing trend. The GnRH immunoreactive products in the abomasum reached the peak at 60 days old and the other parts at 90 days old. GnRHR immunoreactive products were found in gastric fundus gland gastric fovea epithelial cells and parietal cells, small intestinal villus epithelial cells and duodenal gland epithelial cells, large intestine mucosal epithelial cells, and 30 days after 30 days of age. The distribution of glandular epithelial cells in the large intestine gland also appeared, and then maintained a rapid growth trend until 90 days of age reached a peak. From day 0 to day 90, the mean integral optical density of GnRH and GnRHR immunoreactive cells increased significantly (P0.01), and remained relatively stable after 90 days of age (P0.05). To sum up, we speculated that the gastrointestinal tract of Jining green goat can also synthesize and secrete GnRH;GnRH through autocrine or paracrine, which may promote the development and maturation of gastrointestinal tract through the mediation of GnRHR.
【學位授予單位】：山東農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S827
本文編號：2265953
[Abstract]:Jining Grey Goat (Jining), which originated from China, is a unique goat breed for lamb skin, which is characterized by four seasons of estrus, early sexual maturity, 3 months old ewe and 4 months old male sheep. The initial estrous period of Jining green goat begins at 3-4 months old, and some of the early developing individuals begin their early estrous period at the age of 2 months. The estrus cycle is 17.50 days 鹵0.50 days, the estrus duration is 1-2 days, and the gestation period is about 147.00 鹵2.50 days, with an average of 146 days. The reproduction rate of Jining green goat is high, and the survival rate of lamb is up to 95%. Its temperament is easy to manage, genetic stability, strong adaptability, tolerance to coarse feeding, has a high economic value, has been listed in the "Chinese Sheep Variety", belong to the excellent local goat breeds. Study on the Aging changes of Gastrointestinal Gonadotropin releasing hormone (GnRH) and Gonadotropin releasing hormone receptor (GnRHR) Distribution in Jining Green Goat provides a way to understand the effects of GnRH and GnRHR on the gastrointestinal function during postnatal development of animals The necessary theoretical basis is given. To investigate the distribution and developmental changes of GnRH and GnRHR in gastrointestinal tract of Jining green goat during postnatal development. The dynamic distribution of GnRH and GnRHR immunoreactive cells in the gastrointestinal tract of Jining green goats on the day of birth (0 days old), 30 days old, 60 days old, 90 days old, 120 days old, 150 days old and 180 days old was detected by immunohistochemical (SABC) method. In the gastrointestinal tract, GnRH and GnRHR immunoreactive products were located in the cytoplasm, yellow or brown, negative in nucleus, and no positive staining in negative control. At 0 days of age, GnRH immunoreactive products were distributed in the basal base of fundus gland in the abomasum. Between villous epithelium of the small intestine and goblet cells of the large intestine, after 30 days of age, the epithelial cells of the small intestine also appeared to be distributed, and the immunoreactive substances in the other parts of the intestine increased obviously and kept the increasing trend. The GnRH immunoreactive products in the abomasum reached the peak at 60 days old and the other parts at 90 days old. GnRHR immunoreactive products were found in gastric fundus gland gastric fovea epithelial cells and parietal cells, small intestinal villus epithelial cells and duodenal gland epithelial cells, large intestine mucosal epithelial cells, and 30 days after 30 days of age. The distribution of glandular epithelial cells in the large intestine gland also appeared, and then maintained a rapid growth trend until 90 days of age reached a peak. From day 0 to day 90, the mean integral optical density of GnRH and GnRHR immunoreactive cells increased significantly (P0.01), and remained relatively stable after 90 days of age (P0.05). To sum up, we speculated that the gastrointestinal tract of Jining green goat can also synthesize and secrete GnRH;GnRH through autocrine or paracrine, which may promote the development and maturation of gastrointestinal tract through the mediation of GnRHR.
【學位授予單位】：山東農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S827
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，本文編號：2265953
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