【摘要】：為了探索銀杏外種皮多糖(GBEP)在雞胚中對新城疫病毒(NDV)的作用,試驗(yàn)采用水提醇沉法獲得多糖(含量為51.9%),然后用不同濃度多糖接種9~11日齡雞胚測定多糖的安全濃度,在此基礎(chǔ)上比較不同給藥方式(先給藥再攻毒、先攻毒再給藥及藥物和病毒混合孵育后再攻毒)對NDV的作用。結(jié)果表明:GBEP在不高于4 mg/枚雞胚時(shí)不影響雞胚的正常發(fā)育。0.8 mg/枚GBEP處理組的胚體發(fā)育、HA血凝價(jià)均與利巴韋林組相近,而攻毒對照組雞胚在48小時(shí)時(shí)全部死亡。先攻毒再給藥組雞胚在48小時(shí)時(shí)出現(xiàn)5枚死亡,60小時(shí)時(shí)全部死亡;先給藥再攻毒組雞胚在同時(shí)期出現(xiàn)1枚死亡,而藥物與病毒混合孵育后再攻毒組未出現(xiàn)死亡,且72小時(shí)時(shí)胚體長度明顯長于攻毒對照組(P0.05)。說明GBEP具有明顯的預(yù)防和直接抗NDV感染雞胚的作用。
[Abstract]:In order to explore the effect of Ginkgo biloba outer coat polysaccharide (GBEP) on Newcastle disease virus (NDV) (NDV) in chicken embryo, the polysaccharide was obtained by water extraction and alcohol precipitation (51.9%), and the safe concentration of polysaccharides was determined by inoculating 9 ~ 11 day-old chicken embryos with different concentrations of polysaccharides. On this basis, the effects of different drug administration methods (first drug administration and then drug attack, first attack on poison and then drug and virus mixed incubation and then attack on virus) on NDV were compared. The results showed that the normal development of chicken embryos was not affected by GBEP when the number of chicken embryos was not more than 4 mg/. The embryo body development of 0.8 mg/ GBEP treatment group was similar to that of ribavirin group, while that of control group died at 48 hours. Five chicken embryos died at 48 hours and all died at 60 hours in the first attack group, and one embryo died in the same period in the first administration group, but there was no death in the second attack group after the drug and virus were incubated. The length of embryo body at 72 hours was significantly longer than that of control group (P0.05). The results showed that GBEP could prevent and directly resist NDV infection in chicken embryos.
【作者單位】： 揚(yáng)州大學(xué)獸醫(yī)學(xué)院;江蘇省動(dòng)物重要疫病與人畜共患病防控協(xié)同中心;
【基金】：江蘇省高校優(yōu)勢學(xué)科建設(shè)工程項(xiàng)目(JS20130601)
【分類號(hào)】：S853.7
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本文編號(hào)：2263686