【摘要】：為確定南寧市吳圩鎮(zhèn)某黑豚鼠養(yǎng)殖場(chǎng)發(fā)生死亡的原因,從發(fā)病黑豚鼠眼分泌物、囊腫及肺中分離到可疑細(xì)菌,經(jīng)形態(tài)觀察、生理生化特性測(cè)定及16S rRNA序列分析,鑒定該分離菌為馬鏈球菌獸疫亞種。經(jīng)小白鼠回歸試驗(yàn)證實(shí),該分離菌可致死小白鼠。分離菌16S rRNA與馬鏈球菌獸疫亞種(AB104843)的同源性最高,達(dá)99.8%。進(jìn)化樹(shù)分析顯示,該分離菌與馬鏈球菌獸疫亞種(AB104843)、馬鏈球菌馬亞種(JQ726495、JQ726496)的遺傳關(guān)系最近,處于同一分支上。藥敏試驗(yàn)顯示,該分離菌對(duì)氨芐青霉素、氨曲南、頭孢拉定、頭孢唑啉、頭孢氨芐、頭孢呋辛、鏈霉素、卡那霉素等高度敏感,對(duì)青霉素、亞胺培南、四環(huán)素、多西環(huán)素等不敏感。結(jié)果表明,馬鏈球菌獸疫亞種16-58是南寧市吳圩鎮(zhèn)某黑豚鼠養(yǎng)殖場(chǎng)豚鼠致死的病原菌。
[Abstract]:In order to determine the cause of death in a black guinea pig farm in Wuwei Town, Nanning City, suspicious bacteria were isolated from the eye secretion, cyst and lung of the infected black guinea pig. The morphology, physiological and biochemical characteristics and 16s rRNA sequence analysis were analyzed. The isolated strain was identified as Streptococcus equi subspecies. It was confirmed by mouse regression test that the isolated strain could kill mice. The 16s rRNA and AB104843 of Streptococcus equi showed the highest homology (99.8%). Phylogenetic tree analysis showed that the isolate had the most recent genetic relationship with Streptococcus equine (AB104843) and Maya strain (JQ726495,JQ726496), and was located at the same branch. The drug sensitivity test showed that the isolated strain was highly sensitive to ampicillin, aztreonam, cefradine, cefazolin, cefalexin, cefuroxime, streptomycin, kanamycin, penicillin, imipenem, tetracycline, etc. Doxycycline is insensitive. The results showed that Streptococcus equi 16-58 was the pathogen of death in a black guinea pig farm in Wuwei Town Nanning City.
【作者單位】： 廣西壯族自治區(qū)獸醫(yī)研究所廣西獸醫(yī)生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：廣西水產(chǎn)畜牧局科技推廣應(yīng)用項(xiàng)目(桂漁牧科201452004) 廣西基本科研業(yè)務(wù)費(fèi)專(zhuān)項(xiàng)(桂科專(zhuān)項(xiàng)15-4)
【分類(lèi)號(hào)】：S852.611

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