2011-2014年河南省雞群馬立克氏病病毒流行株的分子進(jìn)化及基因重組分析
發(fā)布時(shí)間：2018-09-18 18:49
【摘要】：馬立克氏病病毒(Marek’s disease virus,MDV)是少數(shù)感染自然宿主后,可誘發(fā)淋巴瘤的禽皰疹病毒之一,其感染引起的雞馬立克氏病(Marek’s disease,MD)也是最早可以用抗病毒疫苗來(lái)預(yù)防腫瘤發(fā)生的疾病。為了解雞MDV流行毒株的分子特征,本研究對(duì)2012-2014年河南省發(fā)病雞群的臨床病料進(jìn)行了采集,并對(duì)MDV 132 bp重復(fù)序列進(jìn)行了PCR擴(kuò)增,同時(shí)對(duì)meq、gE和gI基因進(jìn)行了克隆和序列測(cè)定。結(jié)果表明,9份病料中均可擴(kuò)增出與132bp雙拷貝大小相符的PCR主產(chǎn)物;meq、gE和gI基因的核苷酸序列同源性與國(guó)內(nèi)外參考毒株相比,分別為98.5%~100%、98.8%~100%和98.0%~100%;基于這些基因構(gòu)建系統(tǒng)發(fā)生樹(shù)的分析結(jié)果表明,與美國(guó)、澳大利亞、日本以及印度的MDV分離株相比,目前河南雞群中流行的MDV與此前分離的河南流行株進(jìn)化關(guān)系較近,并且與其他國(guó)內(nèi)分離株共同形成一個(gè)較大的獨(dú)立進(jìn)化分支。除了MDV,禽網(wǎng)狀內(nèi)皮增生癥病毒(Reticuloendotheliosis virus,REV)也是導(dǎo)致家禽免疫抑制性疾病和腫瘤病最重要的病原之一。由于MDV和REV具有相似的組織嗜性,�；旌细腥居谕浑u群,因此這兩種病毒的共感染對(duì)家禽養(yǎng)殖業(yè)危害巨大。此前研究表明,MDV基因組中可人工或自然重組REV的部分基因組片段,如長(zhǎng)末端重復(fù)序列(long terminal repeat,LTR),這種重組對(duì)MDV的病原學(xué)特性可能具有重要影響。為了解國(guó)內(nèi)雞群MDV和REV的共感染和自然重組狀況,本研究對(duì)分離自河南商品蛋雞群的17個(gè)MDV分離株進(jìn)行了分析,結(jié)果發(fā)現(xiàn)其中HNGS206和HNXZ103的病毒基因組中存在REV-LTR的重組插入。進(jìn)一步的研究表明這兩個(gè)毒株不是MDV與REV自然重組的流行毒株,其基因組中LTR的插入可能發(fā)生于臨床病例雞共感染后的病毒分離培養(yǎng)過(guò)程中。本研究的結(jié)果提示河南雞群中MDV和REV的共感染較為普遍,但MDV與REV自然重組毒株的流行狀況仍需要進(jìn)一步的流行病學(xué)監(jiān)測(cè)。
[Abstract]:Marek's disease virus (Marek's disease virus,MDV) is one of the few avian herpes virus that can induce lymphoma after it is infected with natural host. The chicken Marek's disease (Marek's disease,MD) caused by Marek's disease is also the first disease that can be prevented by antiviral vaccine. In order to understand the molecular characteristics of chicken MDV epidemic strains, the clinical samples of chickens infected in Henan Province from 2012 to 2014 were collected, the repeat sequence of MDV 132 bp was amplified by PCR, and the genes of meq,gE and gI were cloned and sequenced. The results showed that the nucleotide sequence homology of the major PCR products, mequger E and gI, which were identical to the 132bp double copy size, could be amplified from 9 samples of the disease, compared with the reference strains at home and abroad. The analysis of the phylogenetic tree based on these genes showed that compared with the MDV isolates from the United States, Australia, Japan and India, At present, the MDV prevalent in Henan chicken herd is closely related to the evolution of previously isolated Henan strains and forms a large independent evolutionary branch with other domestic isolates. In addition to MDV, avian reticuloendotheliosis virus (Reticuloendotheliosis virus,REV), it is also one of the most important pathogens leading to immunosuppressive diseases and tumor diseases in poultry. Because MDV and REV have similar tissue preference, they are often mixed infected in the same chicken herd, so the co-infection of these two viruses is harmful to poultry industry. Previous studies have shown that part of the genome of REV that can be recombined artificially or naturally, such as long terminal repeat sequence (long terminal repeat,LTR), may have an important effect on the etiological characteristics of MDV. In order to understand the co-infection and natural recombination of MDV and REV in domestic chickens, 17 MDV isolates isolated from Henan commercial laying hens were analyzed. The results showed that the recombinant insertion of REV-LTR was found in the viral genomes of HNGS206 and HNXZ103. Further studies showed that these two strains were not the natural recombination of MDV and REV, and the insertion of LTR in the genome of the two strains might occur during the isolation and culture of the virus after co-infection in clinical chicken. The results of this study suggest that co-infection of MDV and REV is common in Henan chickens, but the prevalence of natural recombinant strains of MDV and REV is still in need of further epidemiological monitoring.
【學(xué)位授予單位】：河南農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S852.65
[Abstract]:Marek's disease virus (Marek's disease virus,MDV) is one of the few avian herpes virus that can induce lymphoma after it is infected with natural host. The chicken Marek's disease (Marek's disease,MD) caused by Marek's disease is also the first disease that can be prevented by antiviral vaccine. In order to understand the molecular characteristics of chicken MDV epidemic strains, the clinical samples of chickens infected in Henan Province from 2012 to 2014 were collected, the repeat sequence of MDV 132 bp was amplified by PCR, and the genes of meq,gE and gI were cloned and sequenced. The results showed that the nucleotide sequence homology of the major PCR products, mequger E and gI, which were identical to the 132bp double copy size, could be amplified from 9 samples of the disease, compared with the reference strains at home and abroad. The analysis of the phylogenetic tree based on these genes showed that compared with the MDV isolates from the United States, Australia, Japan and India, At present, the MDV prevalent in Henan chicken herd is closely related to the evolution of previously isolated Henan strains and forms a large independent evolutionary branch with other domestic isolates. In addition to MDV, avian reticuloendotheliosis virus (Reticuloendotheliosis virus,REV), it is also one of the most important pathogens leading to immunosuppressive diseases and tumor diseases in poultry. Because MDV and REV have similar tissue preference, they are often mixed infected in the same chicken herd, so the co-infection of these two viruses is harmful to poultry industry. Previous studies have shown that part of the genome of REV that can be recombined artificially or naturally, such as long terminal repeat sequence (long terminal repeat,LTR), may have an important effect on the etiological characteristics of MDV. In order to understand the co-infection and natural recombination of MDV and REV in domestic chickens, 17 MDV isolates isolated from Henan commercial laying hens were analyzed. The results showed that the recombinant insertion of REV-LTR was found in the viral genomes of HNGS206 and HNXZ103. Further studies showed that these two strains were not the natural recombination of MDV and REV, and the insertion of LTR in the genome of the two strains might occur during the isolation and culture of the virus after co-infection in clinical chicken. The results of this study suggest that co-infection of MDV and REV is common in Henan chickens, but the prevalence of natural recombinant strains of MDV and REV is still in need of further epidemiological monitoring.
【學(xué)位授予單位】：河南農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S852.65
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