【摘要】：江西省某豬場育肥豬群出現(xiàn)高熱、呼吸困難和死亡等癥狀,為確定疫病病原,本實驗室從發(fā)病豬場隨機采取20份病死豬血樣和組織,用RT-PCR的方法進行病原檢測。結(jié)果顯示,19份臨床樣品呈現(xiàn)豬繁殖與呼吸綜合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)陽性。隨后對病死豬肺組織進行病毒分離,結(jié)果顯示分離的第1代病毒感染后48 h即可出現(xiàn)典型的致細胞病變效應(yīng)(cytopathic effect,CPE),對傳至第5代的病毒進行RT-PCR檢測和間接免疫熒光鑒定,結(jié)果證實我們分離獲得的病毒為PRRSV,命名為JX14T2。將JX14T2 P5代次的病毒與高致病性PRRSV強、弱毒代表株進行生長特性比較分析,結(jié)果顯示,JX14T2在Marc-145細胞上形成的細胞病變、病毒噬斑形態(tài)以及病毒增殖動態(tài)均與HP-PRRSV毒株相似。對JX14T2進行全長基因測序和序列比較分析,結(jié)果表明JX14T2基因組全長14 960 bp,屬于北美型毒株,其NSP2除存在的1+29個氨基酸的缺失而外,在598~717 aa還存在120個氨基酸的缺失,與HP-PRRSV親緣關(guān)系較近。本研究結(jié)果為掌握PRRSV流行情況以及致病機制提供了科學(xué)依據(jù)。
[Abstract]:High fever, dyspnea and death were found in fattening pigs at a pig farm in Jiangxi Province. In order to determine the pathogen of epidemic disease, 20 blood samples and tissues of dead pigs were randomly collected from the pig farm. The pathogen was detected by RT-PCR method. The results showed that 19 clinical samples were positive for porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus,PRRSV). The virus was isolated from the lung tissue of the dead pigs. The results showed that typical cytopathic effect (cytopathic effect,CPE) appeared 48 hours after the first generation virus was infected. The virus transmitted to the fifth generation was detected by RT-PCR and identified by indirect immunofluorescence. The results confirmed that the virus we isolated was named JX14T2. for PRRSV,. The growth characteristics of JX14T2 P5 virus were compared with those of the representative strain of high pathogenicity PRRSV and attenuated virus. The results showed that the cytopathic effect of JX14T2 on Marc-145 cells, the morphology of virus plaque and the dynamic of virus proliferation were similar to those of HP-PRRSV virus strain. The full-length gene sequencing and sequence comparison analysis of JX14T2 showed that the full-length 14960 bp, of JX14T2 was a North American virus strain. In addition to the deletion of 129 amino acids in NSP2, there were 120 amino acid deletions in 598 aa. The relationship with HP-PRRSV was close. The results of this study provide a scientific basis for mastering the prevalence and pathogenesis of PRRSV.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院上海獸醫(yī)研究所;
【基金】：973計劃(2014CB542702) 國家科技支撐計劃課題(2015BAD12B01-1) 國家生豬現(xiàn)代產(chǎn)業(yè)技術(shù)體系項目(CARS-36 ) 國家自然科學(xué)基金(31302098、31300140、31502072) 上海市閔行區(qū)人才發(fā)展專項資金
【分類號】：S852.651

【參考文獻】

相關(guān)期刊論文 前1條

1 周艷君;田志軍;劉金霞;安同慶;彭金美;薛強;蔡雪輝;于海;李國新;劉娣;童光志;王云峰;王玫;;豬繁殖與呼吸綜合征病毒M蛋白B細胞抗原表位的鑒定[J];中國預(yù)防獸醫(yī)學(xué)報;2009年04期

【共引文獻】

相關(guān)期刊論文 前5條

1 王鑫;楊德強;張文超;姜一峰;虞凌雪;楊莘;高飛;李麗薇;黃勤峰;童光志;周艷君;;豬繁殖與呼吸綜合征病毒JX14T2毒株的分離與鑒定[J];中國動物傳染病學(xué)報;2016年02期

2 鄧靜;袁明銘;文心田;黃小波;曹三杰;文翼平;伍銳;;豬繁殖與呼吸綜合征病毒M基因的截短表達及間接ELISA檢測方法的建立[J];中國獸醫(yī)科學(xué);2014年07期

3 王振華;曹曉涵;宋禾;王迪;曾憲垠;;豬繁殖與呼吸綜合征病毒的危害及免疫策略[J];中國畜牧獸醫(yī);2014年02期

4 李瑩;郭萌;宋文哲;;多表位腫瘤疫苗的研究進展[J];中國新藥與臨床雜志;2012年10期

5 宋帥;李春玲;賈愛卿;楊冬霞;李淼;;抗原表位研究方法進展[J];動物醫(yī)學(xué)進展;2010年12期

【二級參考文獻】

相關(guān)期刊論文 前2條

1 童光志;周艷君;郝曉芳;田志軍;仇華吉;彭金美;安同慶;;高致病性豬繁殖與呼吸綜合征病毒的分離鑒定及其分子流行病學(xué)分析[J];中國預(yù)防獸醫(yī)學(xué)報;2007年05期

2 郭寶清,陳章水,劉文興,崔益洙;從疑似PRRS流產(chǎn)胎兒分離PRRSV的研究[J];中國畜禽傳染病;1996年02期

，

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