【摘要】：江西省某豬場育肥豬群出現高熱、呼吸困難和死亡等癥狀,為確定疫病病原,本實驗室從發(fā)病豬場隨機采取20份病死豬血樣和組織,用RT-PCR的方法進行病原檢測。結果顯示,19份臨床樣品呈現豬繁殖與呼吸綜合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)陽性。隨后對病死豬肺組織進行病毒分離,結果顯示分離的第1代病毒感染后48 h即可出現典型的致細胞病變效應(cytopathic effect,CPE),對傳至第5代的病毒進行RT-PCR檢測和間接免疫熒光鑒定,結果證實我們分離獲得的病毒為PRRSV,命名為JX14T2。將JX14T2 P5代次的病毒與高致病性PRRSV強、弱毒代表株進行生長特性比較分析,結果顯示,JX14T2在Marc-145細胞上形成的細胞病變、病毒噬斑形態(tài)以及病毒增殖動態(tài)均與HP-PRRSV毒株相似。對JX14T2進行全長基因測序和序列比較分析,結果表明JX14T2基因組全長14 960 bp,屬于北美型毒株,其NSP2除存在的1+29個氨基酸的缺失而外,在598~717 aa還存在120個氨基酸的缺失,與HP-PRRSV親緣關系較近。本研究結果為掌握PRRSV流行情況以及致病機制提供了科學依據。
[Abstract]:High fever, dyspnea and death were found in fattening pigs at a pig farm in Jiangxi Province. In order to determine the pathogen of epidemic disease, 20 blood samples and tissues of dead pigs were randomly collected from the pig farm. The pathogen was detected by RT-PCR method. The results showed that 19 clinical samples were positive for porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus,PRRSV). The virus was isolated from the lung tissue of the dead pigs. The results showed that typical cytopathic effect (cytopathic effect,CPE) appeared 48 hours after the first generation virus was infected. The virus transmitted to the fifth generation was detected by RT-PCR and identified by indirect immunofluorescence. The results confirmed that the virus we isolated was named JX14T2. for PRRSV,. The growth characteristics of JX14T2 P5 virus were compared with those of the representative strain of high pathogenicity PRRSV and attenuated virus. The results showed that the cytopathic effect of JX14T2 on Marc-145 cells, the morphology of virus plaque and the dynamic of virus proliferation were similar to those of HP-PRRSV virus strain. The full-length gene sequencing and sequence comparison analysis of JX14T2 showed that the full-length 14960 bp, of JX14T2 was a North American virus strain. In addition to the deletion of 129 amino acids in NSP2, there were 120 amino acid deletions in 598 aa. The relationship with HP-PRRSV was close. The results of this study provide a scientific basis for mastering the prevalence and pathogenesis of PRRSV.
【作者單位】： 中國農業(yè)科學院上海獸醫(yī)研究所;
【基金】：973計劃(2014CB542702) 國家科技支撐計劃課題(2015BAD12B01-1) 國家生豬現代產業(yè)技術體系項目(CARS-36 ) 國家自然科學基金(31302098、31300140、31502072) 上海市閔行區(qū)人才發(fā)展專項資金
【分類號】：S852.651

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