【摘要】：口蹄疫(FMD),豬瘟(CSF)和高致病性藍(lán)耳病(HP-PRRS)是豬的三種重大疾病,農(nóng)業(yè)部將其三種豬傳染病列為強(qiáng)制免疫病種,為保證三種疫苗的免疫效果,各地豬場(chǎng)均采取三種疫苗分別多次注射的免疫程序,這不僅增加了防疫人員的勞動(dòng)力,還增加了疫苗免疫與豬群的免疫應(yīng)激,影響其生產(chǎn)性能,因此三種疫苗是否可以同時(shí)免疫,一直是養(yǎng)豬業(yè)長(zhǎng)期關(guān)注和期待的免疫技術(shù)問(wèn)題。為檢測(cè)和觀察三種疫苗聯(lián)合免疫仔豬后對(duì)其三種免疫抗體效價(jià)、仔豬副反應(yīng)、日增重及成活率的影響,本研究選擇某規(guī)�；i場(chǎng)21-30日齡仔豬150頭,分為五個(gè)組,即A組(FMD、CSF、HP-PRRS三種疫苗分批單獨(dú)免疫)、B組(CSF+PRRS同時(shí)免疫)、C組(CSF+FMD同時(shí)免疫)、D組(FMD+PRRS同時(shí)免疫)、E組(CSF+FMD+PRRS同時(shí)免疫),30日齡首免,30天后二免。在首免后30天,二免后30、60、90天分別采用正向間接血凝試驗(yàn)(IHA)檢測(cè)血清中CSF和FMD抗體,ELISA檢測(cè)血清中PRRS抗體,并對(duì)各組免疫仔豬首免之前和每次檢測(cè)抗體時(shí)進(jìn)行稱重統(tǒng)計(jì)其日增重及成活率,研究結(jié)果如下:1、采用21日齡免疫HP-PRRS,7天后再進(jìn)行CSF和FMD兩種疫苗同時(shí)分點(diǎn)注射的C組,其首免后30天CSF和FMD抗體滴度略低于單獨(dú)免疫組(A組)但高于B、D組,在二免后30天和60天時(shí)兩種抗體滴度均高于單獨(dú)免疫組(A組)、B組、D組,結(jié)果表明,CSF+FMD兩種疫苗聯(lián)合免疫不影響其兩種疫苗的免疫效果。2、采用CSF+FMD+PRRS三種疫苗聯(lián)合免疫組(E組),在首免后30天,三種抗體滴度均略低于三種疫苗單獨(dú)免疫組(A組);在二免60天和90天三種抗體均略高于A組,但差異不顯著;三種疫苗聯(lián)合免疫組在免疫后30天、60天、90天三種抗體滴度均高于B組和D組,差異顯著(P0.05),結(jié)果表明,三種疫苗同時(shí)分點(diǎn)聯(lián)合免疫,不影響其各自免疫應(yīng)答,且能取得與單獨(dú)免疫相同的免疫抗體水平。3、各免疫組仔豬副反應(yīng)、日增重和成活率比較,E組免疫仔豬出現(xiàn)一過(guò)性體溫升高,食欲下降者高于其它組,占10%;CSF+FMD組副反應(yīng)比例最小,為3.3%;CSF+FMD組及CSF+FMD+HP-PRRS組成活率高于其它組,分別為89.5%和85.4%;兩組仔豬的日增重也高于其它組。結(jié)果表明,三種疫苗聯(lián)合免疫可引起免疫仔豬出現(xiàn)副反應(yīng)比例增加,但不影響免疫仔豬的日增重及成活率。4、本研究結(jié)果表明,口蹄疫、豬瘟及高致病豬藍(lán)耳病聯(lián)合免疫,或先進(jìn)性豬藍(lán)耳病免疫,7天后進(jìn)行豬瘟及口蹄疫聯(lián)合免疫均能使免疫仔豬產(chǎn)生良好抗體應(yīng)答,且不影響其日增重,節(jié)省大量免疫接種人力,該兩種程序均可供不同規(guī)模養(yǎng)豬場(chǎng)參考與借鑒。
[Abstract]:Foot-and-mouth disease (FMD), (CSF) and highly pathogenic blue ear disease (HP-PRRS) are three major diseases in pigs. The Ministry of Agriculture lists three kinds of swine infectious diseases as compulsory immunizations, in order to ensure the immune effect of the three vaccines. All local pig farms have adopted immunization procedures of three kinds of vaccines, which not only increase the labor force of epidemic prevention personnel, but also increase vaccine immunization and the immune stress of pigs, thus affecting their production performance. Therefore, whether the three vaccines can be immunized at the same time has been a long-term concern and expectation of the pig industry. In order to detect and observe the effects of combined immunization of three vaccines on the antibody titers, side effects, daily gain and survival rate of piglets, 150 piglets aged 21-30 days were selected from a large scale pig farm and divided into five groups. Group A (three FMD,CSF,HP-PRRS vaccines were immunized in batches) (CSF PRRS was immunized in group B (CSF FMD in group C was immunized at the same time) (FMD PRRS in group D was immunized simultaneously) (CSF FMD PRRS in group E was immunized at the same time) (CSF FMD PRRS was immunized for the first time at 30 days of age and then was immunized twice after 30 days of age. The positive indirect hemagglutination test (IHA) was used to detect the CSF and FMD antibodies in the serum by Elisa, and the PRRS antibody in the serum was detected by Elisa at 30 days after the first immunization and 30 days after the second immunization. The daily gain and survival rate of piglets were calculated by weighing before first immunization and at each time of antibody detection. The results were as follows: 1. CSF and FMD vaccine were injected into group C after 21 days of HP-PRRS,7 immunization. The titers of CSF and FMD antibody were slightly lower in group A than those in group A but higher than those in group D at 30 and 60 days after the first immunization, and the titers of both antibodies were higher than those in group B and group D at 30 and 60 days after the first immunization. The results showed that the combined immunization of the two FMD vaccines did not affect the immune effect of the two vaccines. Group E was immunized with CSF FMD PRRS vaccine for 30 days after the first immunization. The titers of the three antibodies were slightly lower than those of the three vaccines alone (group A), and the titers of the three antibodies were slightly higher than those of group A on day 60 and 90, but the difference was not significant. The titers of the three kinds of antibodies in the combined immunization group were significantly higher than those in group B and group D (P0.05). The results showed that the three vaccines were immunized at the same time and their immune responses were not affected. The immune antibody level was the same as that of single immunization. The side effects, daily gain and survival rate of piglets in each immunized group were higher than those in other groups, and the proportion of side effects in group E was the lowest. The proportion of side effects in group E was higher than that in other groups, and that in group E was higher than that in other groups. The survival rate of FMD group and CSF FMD HP-PRRS group was higher than that of other groups (89.5% and 85.4%, respectively), and the daily gain of piglets in both groups was higher than that in other groups. The results showed that combined immunization of three vaccines could increase the proportion of side effects of immunized piglets, but had no effect on the daily gain and survival rate of immunized piglets. The results showed that the combined immunization of foot-and-mouth disease, hog fever and high pathogenic swine blue ear disease. Or advanced swine blue ear disease immunization with hog fever and foot-and-mouth disease combined immunization 7 days after immunization can make the piglets immune to produce a good antibody response, and do not affect its daily weight, save a large number of immunization manpower. The two procedures can be used for reference and reference in different scale pig farms.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.28

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