【摘要】：為了提高纖維素類有機(jī)質(zhì)的降解率并了解牛瘤胃內(nèi)微生物的種類及功能,從瘤胃液中以不同初始pH為基礎(chǔ)定向培育穩(wěn)定高效的降解群落,利用PCR-DGGE技術(shù)對菌落結(jié)構(gòu)及菌種功能進(jìn)行分析。結(jié)果表明,該菌群對以5g/L微晶纖維素為碳源的發(fā)酵液的利用率最高,達(dá)到83%;在pH 6.5時獲得了較高的產(chǎn)氫量(178.2mL/L),此降解效率及產(chǎn)氫量均高于已報道的纖維素降解菌群。對群落結(jié)構(gòu)分析發(fā)現(xiàn),傳代馴化4次后群落結(jié)構(gòu)基本穩(wěn)定,此群落中含有的菌種主要包括:具有纖維素降解功能的瘤胃菌(Ruminococcaceae),具有產(chǎn)氫能力細(xì)桿菌(Anaerofilum)以及降解纖維素并產(chǎn)生氫氣的腸球菌(Enterococcus)、梭菌類(Clostridium)和腸桿菌(Enterobacter cloacae)。
[Abstract]:In order to improve the degradation rate of cellulose organic matter and understand the species and function of bovine rumen microorganisms, stable and efficient degradation communities were cultivated from rumen fluid based on different initial pH. The colony structure and strain function were analyzed by PCR-DGGE technique. The results showed that the highest utilization rate of the fermentation broth with 5g/L microcrystalline cellulose as carbon source was reached, and the higher hydrogen production (178.2mL/L) was obtained at pH 6.5, which was higher than that of the reported cellulose degrading bacteria. The results of community structure analysis showed that the community structure was stable after 4 times acclimation. The species contained in the community mainly include: cellulose-degrading rumen bacteria (Ruminococcaceae), has hydrogen production ability of (Anaerofilum), cellulose degradation and hydrogen production of Clostridium enterococcus (Enterococcus), (Clostridium) and Enterobacter (Enterobacter cloacae).
【作者單位】： 哈爾濱工業(yè)大學(xué)市政環(huán)境工程學(xué)院;
【基金】：國家自然科學(xué)基金項目(31470233)
【分類號】：S852.6

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