【摘要】：為調(diào)查寵物狗體表角蛋白降解真菌的物種多樣性,從3個(gè)不同生境寵物狗的耳背、腹部、臉頰、背部、趾甲和后腿內(nèi)側(cè)共6個(gè)具有代表性的部位,采用以雞羽毛粉為唯一碳、氮源的選擇性培養(yǎng)基分離到能分解角蛋白的真菌共1 100株。通過形態(tài)學(xué)特征、生物條碼數(shù)據(jù)系統(tǒng)(Barcode of Life Data Systems)和分子系統(tǒng)學(xué)分析等多途徑方法,將這些菌株鑒定為26屬52種。結(jié)合文獻(xiàn)資料和ISHAM(International Society for Human and Animal Mycology)r DNA-ITS數(shù)據(jù)庫的分析結(jié)果表明,交鏈格孢Alternaria alternata、暗孢節(jié)菱孢Arthrinium phaeospermum、黃曲霉Aspergillus flavus、球毛殼菌Chaetomium globosum、球孢芽枝霉Cladosporium sphaerospermum、芽枝狀枝孢霉Cladosporium cladosporioides、細(xì)小枝孢霉Cladosporium tenuissimum、擬粗壯彎孢Curvularia pseudorobusta、黑附球菌Epicoccum cf.nigrum和普通裂褶菌Schizophyllum commune共隸屬于8屬的10個(gè)種有可能對(duì)人類具有潛在的感染風(fēng)險(xiǎn)。寵物狗體表存在較為豐富的角蛋白降解真菌,包含有一批潛在的非皮膚癬菌類致病真菌,應(yīng)引起寵物狗愛好者和相關(guān)親密接觸人員的足夠重視!
[Abstract]:In order to investigate the species diversity of fungi degrading by keratin on the body surface of pet dogs, six representative parts of the ear, abdomen, cheek, back, toenail and inner side of the hind legs of the dogs in three different habitats were selected. Chicken feather powder was used as the sole carbon. A total of 1 100 strains of fungi capable of decomposing keratin were isolated from nitrogen selective medium. These strains were identified as 52 species belonging to 26 genera by morphological characteristics, (Barcode of Life Data Systems) and molecular phylogenetic analysis. The results of literature and ISHAM (International Society for Human and Animal Mycology) r DNA-ITS database analysis show that, Alternaria Alternaria alternata, Arthrinium phaeospermum, Arthrinium phaeospermum, Aspergillus flavus, Chaetomium globosum, Cladosporium sphaerospermum, Cladosporium cladosporioides, Cladosporium tenuissimum, Curvularia pseudorobusta, Curvularia pseudorobusta, Epicoccum cf.nigrum, and Schizophyllum commune, a common fructus. Ten species in 8 genera may have a potential infection risk to humans. There are abundant keratin degrading fungi on the body surface of pet dogs, including a number of potential non-dermatophytes pathogenic fungi, which should be paid enough attention by pet dog lovers and related close contacts.
【作者單位】： 貴州大學(xué)生命科學(xué)學(xué)院真菌資源研究所;
【基金】：科技部基礎(chǔ)專項(xiàng)項(xiàng)目子項(xiàng)目(2013FY110400) 國家自然科學(xué)基金(31460010 貴州省優(yōu)秀青年科技人才培養(yǎng)對(duì)象專項(xiàng)資金項(xiàng)目[黔科合人字(2013)05號(hào)]~~
【分類號(hào)】：S829.2;S852.6

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