【摘要】：為探明貴州某雞場患病雞的細(xì)菌感染種類、耐藥性及致病性等情況,本試驗(yàn)對送檢的4只病雞進(jìn)行細(xì)菌分離培養(yǎng),并對分離所得的細(xì)菌進(jìn)行革蘭氏染色鏡檢、生化試驗(yàn)、藥敏試驗(yàn)、16SrDNA分子序列及動物感染試驗(yàn)等分析。結(jié)果顯示,成功分離到了2株菌落形態(tài)不一的菌株,分別為革蘭氏陰性桿菌與陽性球菌,根據(jù)分離地點(diǎn)和時間將其分別命名為GZHX2016-1及GZHX2016-2。GZHX2016-1為大腸桿菌,具有多重耐藥性;GZHX2016-2為模仿葡萄球菌,血漿凝固酶陰性,對多種常用抗生素耐藥。GZHX2016-1的16SrDNA與大腸桿菌(GenBank登錄號:CP007442.1、CP014667、KT156725.1等)同源性達(dá)99.5%,GZHX2016-2與葡萄球菌(GenBank登錄號:HM140412.1、AM944030.1、KM877513.1等)同源性達(dá)97.9%。GZHX2016-1與GZHX2016-2對小鼠都有致病性,GZHX2016-1的最小致死量為1.12×10~8 CFU,GZHX2016-2為3.20×10~7 CFU。綜上所述,本試驗(yàn)成功從送檢雞中分離出1株致病性大腸桿菌和1株致病性血漿凝固酶陰性模仿葡萄球菌,該雞場雞群存在大腸桿菌與葡萄球菌混合感染的情況,且該雞場感染細(xì)菌對多種抗生素耐藥,應(yīng)采取有效措施控制細(xì)菌在雞群中的污染并限制抗生素在養(yǎng)雞過程中的使用,以減少細(xì)菌耐藥性的產(chǎn)生,保障雞肉及雞肉制品的食品安全。
[Abstract]:In order to find out the species, drug resistance and pathogenicity of bacterial infection in diseased chickens from a chicken farm in Guizhou, four diseased chickens were isolated and cultured. The isolated bacteria were analyzed by Gram staining microscopy, biochemical test, drug sensitivity test, 16SrDNA molecular sequence and animal infection test. Two strains with different colony morphology, Gram-negative bacilli and Gram-positive cocci, were named GZHX2016-1 and GZHX2016-2. The homology between 16SrDNA and Escherichia coli (GenBank login number: CP007442.1, CP014667, KT156725.1, etc.) was 99.5%. GZHX2016-2 and Staphylococcus (GenBank login number: HM140412.1, AM944030.1, KM877513.1, etc.) were 97.9%. Both GZHX2016-1 and GZHX2016-2 were pathogenic to mice. The minimum lethal dose of GZHX2016-1 was 1.12 *10~8 CFU and GZHX2016-1 was 1. In summary, 1 strain of pathogenic E. coli and 1 strain of pathogenic plasma coagulase negative Staphylococcus mimicry were successfully isolated from chickens. There were mixed infections of E. coli and Staphylococcus in chickens of the chicken farm, and the bacteria were resistant to many antibiotics. Effective measures should be taken to control the infection. Bacteria contamination in chicken flocks and the use of antibiotics in chicken breeding process are restricted to reduce bacterial resistance and ensure the safety of chicken and chicken products.
【作者單位】： 貴州大學(xué)動物科學(xué)學(xué)院;貴州省動物疫病與獸醫(yī)公共衛(wèi)生重點(diǎn)實(shí)驗(yàn)室;
【基金】：貴州大學(xué)博士基金(貴大人基合字201312號);貴州大學(xué)“本科教學(xué)工程”項(xiàng)目(JKSP2013004) 貴州省科技計(jì)劃課題聯(lián)合基金(黔科合LH字20147666號) 貴州省科學(xué)技術(shù)基金(黔科合J字20152046號) 貴州省科技創(chuàng)新人才團(tuán)隊(duì)項(xiàng)目(黔科合人才團(tuán)隊(duì)20154016)
【分類號】：S858.31

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