不同遺傳背景豬群體黏病毒耐藥蛋白1基因多態(tài)性分析
發(fā)布時間：2018-08-25 15:46
【摘要】：為探討不同遺傳背景豬黏病毒耐藥蛋白1(myxovirus resistance,Mx1)基因的遺傳多態(tài)性,本試驗采用RT-PCR方法擴增克隆出豬Mx1基因并進行生物信息學分析,采用高分辨率熔解曲線法(HRM)對民豬、長白山野豬與民豬雜交豬(簡稱野雜豬)和大白豬3個群體Mx1基因外顯子2區(qū)多態(tài)性進行分析。結果顯示,從民豬及野雜豬中成功克隆出4條Mx1基因mRNA序列。該序列含有1個1 992bp的完整開放閱讀框(ORF),編碼663個氨基酸。比較發(fā)現4條Mx1基因序列中共含有30個SNPs位點,其中17個SNPs位點引起了氨基酸序列的變異。功能結構域分析發(fā)現,大部分氨基酸突變位點位于Mx1基因功能結構域兩側。HRM多態(tài)性分析顯示,Mx1基因外顯子2區(qū)DD基因型作為優(yōu)勢基因型在3個群體中具有最高的基因型頻率,其中大白豬中DD基因型頻率為100%,野雜豬為62.5%,民豬為56.2%�；蚨鄳B(tài)性分析還發(fā)現該區(qū)域存在更多的核苷酸及氨基酸突變位點,表明民豬和野雜豬Mx1基因存在更豐富的基因多態(tài)性。
[Abstract]:In order to investigate the genetic polymorphism of porcine myxovirus resistance protein 1 (myxovirus resistance,Mx1) gene from different genetic backgrounds, porcine Mx1 gene was amplified by RT-PCR method and analyzed by bioinformatics. High resolution fusion curve method (HRM) was used to analyze the genetic polymorphism of porcine myxovirus resistance protein 1 (myxovirus resistance,Mx1) gene. The polymorphism of exon 2 of Mx1 gene in three populations of Changbai Mountain wild boar and Min pig (wild hybrid pig) and big white pig was analyzed. The results showed that four mRNA sequences of Mx1 gene were cloned successfully from Min pig and wild crossbred pig. The sequence contains a 1 992bp complete open reading frame (ORF), encoding 663 amino acids. A total of 30 SNPs loci were found in 4 Mx1 gene sequences, 17 of which caused amino acid sequence variation. The results of functional domain analysis showed that most of the amino acid mutation loci were located at the two sides of the Mx1 gene functional domain. The results showed that the DD genotype of exon 2 of Mx1 gene had the highest genotype frequency among the three populations. The frequency of DD genotypes in large white pigs, wild crossbred pigs and Min pigs were 100, 62.5 and 56.2, respectively. It was also found that there were more nucleotide and amino acid mutation sites in this region, which indicated that the Mx1 gene of Minzhou pig and wild crossbred pig had more polymorphism.
【作者單位】： 吉林省農業(yè)科學院畜牧分院;延邊大學農學院;
【基金】：吉林省重點科技公關項目(20140204061NY)
【分類號】：S828
，
本文編號：2203377
[Abstract]:In order to investigate the genetic polymorphism of porcine myxovirus resistance protein 1 (myxovirus resistance,Mx1) gene from different genetic backgrounds, porcine Mx1 gene was amplified by RT-PCR method and analyzed by bioinformatics. High resolution fusion curve method (HRM) was used to analyze the genetic polymorphism of porcine myxovirus resistance protein 1 (myxovirus resistance,Mx1) gene. The polymorphism of exon 2 of Mx1 gene in three populations of Changbai Mountain wild boar and Min pig (wild hybrid pig) and big white pig was analyzed. The results showed that four mRNA sequences of Mx1 gene were cloned successfully from Min pig and wild crossbred pig. The sequence contains a 1 992bp complete open reading frame (ORF), encoding 663 amino acids. A total of 30 SNPs loci were found in 4 Mx1 gene sequences, 17 of which caused amino acid sequence variation. The results of functional domain analysis showed that most of the amino acid mutation loci were located at the two sides of the Mx1 gene functional domain. The results showed that the DD genotype of exon 2 of Mx1 gene had the highest genotype frequency among the three populations. The frequency of DD genotypes in large white pigs, wild crossbred pigs and Min pigs were 100, 62.5 and 56.2, respectively. It was also found that there were more nucleotide and amino acid mutation sites in this region, which indicated that the Mx1 gene of Minzhou pig and wild crossbred pig had more polymorphism.
【作者單位】： 吉林省農業(yè)科學院畜牧分院;延邊大學農學院;
【基金】：吉林省重點科技公關項目(20140204061NY)
【分類號】：S828
，
本文編號：2203377
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