【摘要】：旨在探明豬細(xì)小病毒樣顆粒(PPV-VLPs)被豬脾樹突狀細(xì)胞(DC)捕獲后,被提呈的方式。首先通過(guò)磁性篩選的方法從非免疫豬和免疫豬的脾分離CD172a+CD11R+DC及CD4-CD8+T細(xì)胞。DC分別與伯氨喹、放線菌酮、氯喹、乳胞素、布雷菲德菌素A及亮抑肽酶、胃酶抑素等作用1h后,再與細(xì)小病毒樣顆粒PPV-VLPs-E290在37℃作用4h,應(yīng)用CD8+T細(xì)胞的細(xì)胞毒性分析檢測(cè)DC對(duì)PPV-VLPs-E290的提呈情況。乳酸脫氫酶(LDH)釋放試驗(yàn)檢測(cè)結(jié)果顯示,伯氨喹及亮抑肽酶對(duì)DC提呈PPV-VLPs-E290的效率無(wú)明顯影響,胃酶抑素部分抑制,而氯喹、放線菌酮、布雷菲德菌素A及乳胞素則可使DC提呈PPV-VLPs-E290的效率明顯下降。結(jié)果表明,DC通過(guò)交叉提呈的方式提呈PPV-VLPs-E290。晚期內(nèi)體的酸性環(huán)境以及蛋白酶的水解均參與了PPV-VLPs-E290的提呈過(guò)程。
[Abstract]:The aim of this study was to identify the way in which porcine parvovirus like particles (PPV-VLPs) were captured by porcine splenic dendritic cells (DC). Firstly, CD172a CD11R DC and CD4-CD8 T cells were isolated from the spleen of non-immunized pigs and immunized pigs by magnetic screening method. The cells were treated with primary aminoquine, actinomycin, chloroquine, lactocytin, Blefedgenin A, brilliant aprotinin and gastric endostatin for 1 h, respectively. After being exposed to parvovirus like particle PPV-VLPs-E290 for 4 h at 37 鈩，

本文編號(hào)：2202889